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Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model

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Abstract

In present study we determined the long term in vivo integration and histological modeling of an in vitro engineered cartilage construct. Tissue engineered autologous cartilagenous tissue was cultured on calcium phosphate cylinders and implanted into osteochondral defects into the femoral condyles in minipigs. Radiological follow-up was performed at 2, 8, 26 and 52 weeks, condyles were harvested 26 and 52 weeks post-implantation. Thickness of cultivated tissue (1.10 ± 0.55 mm) was comparable to in situ cartilage and cells produced in vitro cartilage specific proteins. In vivo, 26 and 52 weeks post-implantation defects were resurfaced with hyaline-like tissue, the implants were well integrated with no gap at the interface between the engineered neocartilage and the adjacent articular cartilage. Synthesis of type II collagen was detected 26 and 52 weeks after implantation. The modified ICRS score increased from 26 to 52 weeks. Histomorphometric evaluation revealed a decrease in cellularity in tissue engineered cartilage from 2.2-fold of native cartilage after 26 weeks to 1.5-fold after 52 weeks. In conclusion, these findings demonstrate the integration and maturation of tissue engineered cartilage pellets attached on a bone substitute carrier implanted in osteochondral defects over a long time.

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References

  1. K. H. PRIDIE, J Bone Joint Surg. Br. 41 (1959) 618

    Google Scholar 

  2. P. C. KREUZ, M. R. STEINWACHS, C. ERGGELET, S. J. KRAUSE, G. KONRAD, M. UHL and N. SUDKAMP, Osteoarthr. Cartil. 14 (2006) 1119

    Article  CAS  Google Scholar 

  3. L. HANGODY and P. FULES, J Bone Joint Surg. Am. 85(Suppl 2) (2003) 25

    Google Scholar 

  4. K. MITHOFER, L. PETERSON, B. R. MANDELBAUM and T. MINAS, Am. J. Sports Med. 33 (2005) 1639

    Article  Google Scholar 

  5. G. BENTLEY, L. C. BIANT, R. W. CARRINGTON, M. AKMAL, A. GOLDBERG, A. M. WILLIAMS, J. A. SKINNER and J. PRINGLE, J Bone Joint Surg. Br. 85 (2003) 223

    Article  CAS  Google Scholar 

  6. U. HORAS, D. PELINKOVIC, G. HERR, T. AIGNER and R. SCHNETTLER, J Bone Joint Surg. Am. 85 (2003) 185

    Google Scholar 

  7. G. KNUTSEN, L. ENGEBRETSEN, T. C. LUDVIGSEN, J. O. DROGSET, T. GRONTVEDT, E. SOLHEIM, T. STRAND, S. ROBERTS, V. ISAKSEN and O. JOHANSEN, J Bone Joint Surg. Am. 86 (2004) 455

    Google Scholar 

  8. J. HUARD, L. YONG, P. HAIRONG and F. H. FU, J. Gene Med. 5 (2003) 93

    Article  Google Scholar 

  9. V. MARTINEK, P. UEBLACKER and A. B. IMHOFF, J Bone Joint Surg. Br. 85 (2003) 782

    CAS  Google Scholar 

  10. E. M. OOMS, J. G. WOLKE, J. P. van der WAERDEN and J. A. JANSEN, J. Biomed. Mater. Res. 61 (2002) 9

    Article  CAS  Google Scholar 

  11. R. P. Del REAL, J. C. G. WOLKE, M. VALLET-REGÍ and J. A. JANSEN, Biomaterials 23 (2002) 3673

    Article  Google Scholar 

  12. M. BUTNARIU-EPHRAT, D. ROBINSON, D. G. MENDES, N. HALPERIN and Z. NEVO, Clin. Orthop. 330 (1996) 234

    Article  Google Scholar 

  13. D. W. JACKSON, P. A. LALOR, H. M. ABERMAN and T. M. SIMON, J Bone Joint Surg. Am. 83 (2001) 53

    Article  Google Scholar 

  14. P. MAINIL-VARLET, F. RIESER, S. GROGAN, W. MUELLER, C. SAAGER and R. P. JAKOB, Osteoarthr. Cartil. 9(Suppl A) (2001) S6

    Article  Google Scholar 

  15. T. GOTTERBARM, W. RICHTER, M. JUNG, S. B. VILEI, P. MAINIL-VARLET, T. YAMASHITA and S. J. BREUSCH, Biomaterials 27 (2006) 3387

    Article  CAS  Google Scholar 

  16. E. M. OOMS, E. A. EGGLEZOS, J. G. C. WOLKE and J. A. JANSEN, Biomaterials 24 (2003) 749

    Article  CAS  Google Scholar 

  17. R. O. C. OREFFO, F. C. M. DRIESSENS, J. A. PLANELL and J. T. TRIFFITT, Tissue Eng. 4 (1998) 293

    Article  CAS  Google Scholar 

  18. S. NAGEL-HEYER, C. GOEPFERT, M. M. MORLOCK and R. POERTNER, Biotechnol. Lett. 27 (2005) 187

    Article  CAS  Google Scholar 

  19. P. C. YAEGER, T. L. MASI, J. L. BUCK de ORTIZ, F. BINETTE, F. TUBO and J. M. MC PHERSON, Exp. Cell Res. 237 (1997) 318

    Article  CAS  Google Scholar 

  20. M. D. BUSCHMANN, Y. A. GLUZBAND, A. J. GRODZINSKY, J. H. KIMURA and E. B. HUNZIKER, J. Orthop. Res. 10 (1992) 745

    Article  CAS  Google Scholar 

  21. M. AMLING, M. HAHN, V. J. WENING, H. J. GROTE and G. DELLING, J Bone Joint Surg. Am. 76 (1994) 1840

    CAS  Google Scholar 

  22. P. MAINIL-VARLET, T. AIGNER, M. BRITTBERG, P. BULLOUGH, A. HOLLANDER, E. HUNZIKER, R. KANDEL, S. NEHRER, K. PRITZKER, S. ROBERTS and E. STAUFFER, J Bone Joint Surg. Am. 85 (2003) 45

    Article  Google Scholar 

  23. X. GUO, C. WANG, C. DUAN, M. DESCAMPS, Q. ZHAO, L. DONG, S. LÜ, K. ANSELME, J. LU and Y. Q. SONG, Tissue Eng. 10 (2004) 1830

    Article  CAS  Google Scholar 

  24. E. M. DARLING and K. A. ATHANASIOU, J. Orthop. Res. 23 (2005) 425

    Article  CAS  Google Scholar 

  25. K. H. FROSCH, A. DRENGK, P. KRAUSE, V. VIERECK, N. MIOSGE, C. WERNER, D. SCHILD, E. K. STÜRMER and K. M. STÜRMER., Biomaterials 27 (2006) 2542

    Article  CAS  Google Scholar 

  26. B. Von RECHENBERG, M. K. AKENS, D. NADLER, P. BITTMANN, K. ZLINSZKY, A. KUTTER, A. R. POOLE and J. A. AUER, Osteoarthr. Cartil. 11 (2003) 265

    Article  Google Scholar 

  27. A. I. VASARA, M. M. HYTTINEN, M. J. LAMMI, P. E. LAMMI, T. K. LANGSJÖ, A. LINDAHL, L. PETERSON, M. KELLOMAKI, Y. T. KONTTINEN, H. F. HELMINEN and I. KIVIRANTA, Calcif. Tissue Int. 74 (2004) 107

    Article  CAS  Google Scholar 

  28. Z. ZHANG, J. M. MCCAFFERY, R. G. S. SPENCER and C. A. FRANCOMANO, J . Orthop. Res. 23 (2005) 433

    Article  Google Scholar 

  29. U. HORAS, D. PELINKOVIC, G. HERR, T. AIGNER and R. SCHNETTLER, J Bone Joint Surg. Am. 85 (2003) 185

    Google Scholar 

  30. J. G. LANE, J. B. MASSIE, S. T. BALL, M. E. AMIEL, A. C. CHEN, W. C. BAE, R. L. SAH and D. AMIEL, Am. J. Sports Med. 32 (2004) 1440

    Article  Google Scholar 

  31. T. AIGNER and J. STOVE, Adv. Drug Deliv. Rev. 55 (2003) 1569

    Article  CAS  Google Scholar 

  32. F. S. HUANG, P. T. SIMONIAN, A. G. NORMAN and J. M. CLARK, Am. J. Sports Med. 32 (2004) 1842

    Article  Google Scholar 

  33. S. ROBERTS, I. W. MCCALL, A. J. DARBY, J. MENAGE, H. EVANS, P. E. HARRISON and J. B. RICHARDSON, Arthritis Res. Ther. 5 (2003) R60

    Article  Google Scholar 

  34. F. ECKSTEIN, Methods Mol. Med. 101 (2004) 191

    Google Scholar 

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Acknowledgments

The authors thank Stephanie Nagel-Heyer, Frank Feyerabend and Jens Schroeder for their technical assistance. This work was supported by the BMBF (03 N 4012).

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Authors and Affiliations

  1. Department of Trauma, Hand and Reconstructive Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany

    J. P. Petersen, P. Ueblacker, K. Baumbach, J. M. Rueger, M. Amling & N. M. Meenen

  2. Experimental Trauma Surgery and Skeletal Biology, Center for Biomechanics, University Medical Center Hamburg-Eppendorf, Lottestrasse 59, 20246, Hamburg, Germany

    J. P. Petersen, P. Ueblacker, J. M. Rueger, M. Amling & N. M. Meenen

  3. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestrasse 15, 21073, Hamburg, Germany

    C. Goepfert & R. Poertner

  4. Department of Biochemistry and Molecular Biology II: Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany

    C. Goepfert & P. Adamietz

  5. Department of Diagnostic and Interventional Radiology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany

    A. Stork

Authors
  1. J. P. Petersen
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  2. P. Ueblacker
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  3. C. Goepfert
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  4. P. Adamietz
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  5. K. Baumbach
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  7. J. M. Rueger
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  8. R. Poertner
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  9. M. Amling
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  10. N. M. Meenen
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Corresponding author

Correspondence to N. M. Meenen.

Additional information

J. P. Petersen, P. Ueblacker, C. Goepfert have contributed equally to this study.

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Petersen, J.P., Ueblacker, P., Goepfert, C. et al. Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model. J Mater Sci: Mater Med 19, 2029–2038 (2008). https://doi.org/10.1007/s10856-007-3291-3

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  • DOI: https://doi.org/10.1007/s10856-007-3291-3

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