Skip to main content
SpringerLink
Log in

Comparative in vitro study of the proliferation and growth of ovine osteoblast-like cells on various alloplastic biomaterials manufactured for augmentation and reconstruction of tissue or bone defects

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

In this in vitro study ovine osteoblast-like cells were cultured on seven different alloplastic biomaterials used for augmentation and for reconstruction of bone defects in dental and craniomaxillofacial surgery. The aim of this study was to examine the growth behaviour (viability, cell density and morphology) of ovine osteoblast-like cells on the investigated biomaterials to get knowledge which biomaterial is qualified to act as a cell carrier system in further in vivo experiments. The biomaterials were either synthetically manufactured or of natural origin. As synthetically manufactured biomaterials Ethisorb®, MakroSorb®, Palacos®R, and PDS® film were used. As biomaterials of natural origin Beriplast®P, Bio-Oss® and Titanmesh were investigated. The cell proliferation and cell colonization were analyzed by a proliferation assay and scanning electron microscopy. Osteoblast-like cells proliferated and attached on all biomaterials, except on Beriplast®. On Ethisorb® the highest cell proliferation rate was measured followed by Palacos®R. Both biomaterials offer suitable growth and proliferation conditions for ovine osteoblast-like cells. The proliferation rates of Bio-Oss®, MakroSorb®, PDS®-film and Titanmesh were low and SEM examinations of these materials showed less spread osteoblast-like cells. The results showed that ovine osteoblast-like cells appear to be sensitive to substrate composition and topography. This in vitro study provides the basis for further in vivo studies using the sheep model to examine the biocompatibility and the long-term interaction between the test material and tissue (bone regeneration).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. S. J. HOLLISTER, R. A. LEVY, T. M. CHU, J. W. HALLORAN and S. E. FEINBERG, Int. J. Oral Maxillofac. Surg. 29 (2000) 67

    Article  CAS  Google Scholar 

  2. R. MALEKZADEH, J. O. HOLLINGER, D. BUCK, D. F. ADAMS and B. S. MCALLISTER, J. Periodontol. 69 (1998) 1256

    CAS  Google Scholar 

  3. M. J. YASZEMSKI, R. G. PAYNE, W. C. HAYES, R. LANGER and A. G. MIKOS, Biomaterials 17 (1996) 175

    Article  CAS  Google Scholar 

  4. C. PERKA, O. SCHULTZ, R. S. SPITZER, K. LINDENHAYN, G. R. BURMESTER and M. SITTINGER, Biomaterials. 21 (2000) 1145

    Article  CAS  Google Scholar 

  5. K. G. MARRA, J. W. SZEM, P. N. KUMTA, P. A. DIMILLA and L. E. WEISS, J. Biomed. Mater. Res. 47 (1999) 324

    Article  CAS  Google Scholar 

  6. K. WHANG, D. C. TSAI, E. K. NAM, M. AITKEN, S. M. SPRAGUE, P. K. PATEL and K. E. HEALY, J. Biomed. Mater. Res. 42 (1998) 491

    Article  CAS  Google Scholar 

  7. A. S. BREITBART, D. A. GRANDE, R. KESSLER, J. T. RYABY, R. J. FITZSIMMONS and R. T. GRANT, Plast. Reconstr. Surg. 101 (1998) 567

    Article  CAS  Google Scholar 

  8. M. S. BLOCK and J. N. KENT, J. Am. Dental Ass. 124 (1993) 139

    CAS  Google Scholar 

  9. G. B. CORNWALL, K. A. THOMAS, A. S. TURNER, W. R. TAYLOR and D. L. WHEELER, Protected Bone Regeneration In An Iliac Crest Animal Model. (Macropore, Inc., San Diego, 2003)

    Google Scholar 

  10. R. SCHMELZEISEN and H. SCHLIEPHAKE, J. Craniomaxillofac. Surg. 26 (1998) 1

    CAS  Google Scholar 

  11. R. SCHMELZEISEN and R. SCHÖN, Int. J. Oral Maxillofac. Implants. 27 (1998) 377

    Article  CAS  Google Scholar 

  12. J. E. HAUSAMEN and F. W. NEUKAM, Eur. Arch. Otorhinolaryngol. 1 (1992) 163

    CAS  Google Scholar 

  13. K. E. SALYER and D. P. TAYLOR, Clin. Plast. Surg. 14 (1987) 27

    CAS  Google Scholar 

  14. S. A. WOLFE, Clin. Plast. Surg. 9 (1982) 539

    CAS  Google Scholar 

  15. D. D. LEE, A. TOFIGHI, M. AIOLOVA, P. CHAKRAVARTHY, A. CATALANO, A. MAJAHAD and D. KNAACK, Clin. Orthop. 367 (1999) 396

    Article  Google Scholar 

  16. G. M. CRANE, S. L. ISHAUG, and A. G. MIKOS, Nat. Med. 1 (1995) 1322

    Article  CAS  Google Scholar 

  17. A. SKOGLUND, P. HISING and C. YOUNG, Int. J. Oral Maxillofac. Implants. 12 (1997) 194

    CAS  Google Scholar 

  18. E. M. YOUNGER and M. W. CHAPMAN, J. Orthoped. Trauma. 3 (1989) 192

    Article  CAS  Google Scholar 

  19. D. GROB, Autologous Bone Grafts. Problems at the Donor Site, edited by M. AEBI, P. REAGAZZONI, Bone Transplantation. (Springer, Berlin, 1989) p. 32

  20. T. W. BAUER and G. F. MUSCHLER, Clin. Orthop. 371 (2000) 10

    Article  Google Scholar 

  21. T. YOSHIKAWA, H. NAKAJIMA, E. YAMADA, M. AKAHANE, Y. DOHI, H. OHGUSHI, S. TAMAI and K. ICHIJIMA, J. Bone Miner. Res. 15 (2000) 1147

    Article  CAS  Google Scholar 

  22. P. DUCHEYNE and Q. QIU, Biomaterials 20 (1999) 2287

    Article  CAS  Google Scholar 

  23. H. E. SCHRATT, G. REGEL, B. KIESEWETTER and H. TSCHERNE, Unfallchirurg 99 (1996) 679

    Article  CAS  Google Scholar 

  24. R. C. THOMSON, A. G. MIKOS, E. BEAHM, J. C. LEMON, W. C. SATTERFIELD, T. B. AUFDEMORTE and M. J. MILLER, Biomaterials 20 (1999) 2007

    Article  CAS  Google Scholar 

  25. G. B. STARK, R. HORCH and E. E. TANCZOS, Biological Matrices And Tissue Reconstruction. (Springer-Verlag, Berlin Heidelberg New York, 1998)

    Google Scholar 

  26. M. WIEDMANN-AL-AHMAD, R. GUTWALD, N.-C. GELLRICH, U. HÜBNER and R. SCHMELZEISEN, J. Mater. Sci. Mater. Med. 16 (2005) 57

    Article  CAS  Google Scholar 

  27. U. SCHNEIDER, Orthopäde 27 (1998) 143

    CAS  Google Scholar 

  28. U. RIPAMONTI, J. CROOKS and D. C. RUEGER, Plast. Reconstr. Surg. 107 (2001) 977

    Article  CAS  Google Scholar 

  29. H. TERHEYDEN, C. KNAK, S. JEPSEN, S. PALMIE and D. R. RUEGER, Int. J. Oral. Maxillofac. Surg. 30 (2001) 373

    Article  CAS  Google Scholar 

  30. O. HANISCH, D. N. TATAKIS, M. D. ROHRER, P. S. WOHRLE, J. M. WOZNEY and U. M. WIESJO, Int. J. Oral Maxillofac. Implants. 12 (1997) 785

    CAS  Google Scholar 

  31. T. UEMURA, J. DONG, Y. WANG, H. KOJIMA, T. SAITO, D. IEJIMA, M. KIKUCHI, J. TANAKA and T. TATEISHI, Biomaterials. 24 (2003) 2277

    Article  CAS  Google Scholar 

  32. R. ZHANG and P. X. MA, J. Biomed. Mater. Res. 44 (1999) 446

    Article  CAS  Google Scholar 

  33. K. J. BURG, S. PORTER and J. F. KELLAM, Biomaterials. 21 (2000) 2347

    Article  CAS  Google Scholar 

  34. S. S. JENSEN, M. AABOE, E. M. PINHOLT, E. HJORTING-HANSEN, F. MELSEN, and I. E. RUYTER, Int. J. Oral Maxillofac. Implants 11 (1996) 55

    CAS  Google Scholar 

  35. D. CAMPOCCIA, C. R. ARCIOLA, M. CERVELLATI, M. C. MALTARELLO and L. MONTANARO, Biomaterials. 24 (2003) 587

    Article  CAS  Google Scholar 

  36. N. R. KÜBLER, Mund Kiefer GesichtsChir. 1 (1997) 2

    Article  Google Scholar 

  37. J. DOBKOWSKI, R. KOLOS, J. KAMINSKI and H. M. KOWALCZYNSKA, J. Biomed. Mater. Res. 47 (1999) 234

    Article  CAS  Google Scholar 

  38. S. L. ISHAUG, G. M. CRANE, M. J. MILLER, A. W. YASKO, M. J. YASZEMSKI and A. G. MIKOS, J. Biomed. Mater. Res. 36 (1997) 17

    Article  CAS  Google Scholar 

  39. K. ANSELME, M. BIGERELLE, B. NOEL, E. DUFRESNE, D. JUDAS, A. LOST and P. HARDOUIN, J. Biomed. Mater. Res. 49 (2000) 155

    Article  CAS  Google Scholar 

  40. B. D. BOYAN, T. W. HUMMERT, D. D. DEAN and Z. SCHWARTZ, Biomaterials 17 (1996) 137

    Article  CAS  Google Scholar 

  41. B. D. BOYAN, V. L. SYLVIA, D. D. DEAN, C. H. LOHMANN, D. L. COCHRAN and Z. SCHWARTZ, Osteologie. 8 (1999) 123

    Google Scholar 

  42. G. LAUER, M. WIEDMANN-AL-AHMAD, J. E. OTTEN, U. HÜBNER, R. SCHMELZEISEN and W. SCHILLI, Biomaterials 22 (2001) 2799

    Article  CAS  Google Scholar 

  43. W. TAN and T. A. DESAI, Tissue Eng. 9 (2003) 255

    Article  CAS  Google Scholar 

  44. J. A. FIALKOV, C. E. HOLY, M. S. SHOICHET and J. E. DAVIES, J. Craniofac. Surg. 14 (2003) 324

    Article  Google Scholar 

  45. P. TORRICELLI, M. FINI, G. GIAVARESI, M. ROCCA, G. PIERINI and R. GIARDANO, J.Biomed. Mater. Res. 52 (2000) 177

    Article  CAS  Google Scholar 

  46. D. W. HUTMACHER, Biomaterials 21 (2000) 2529

    Article  CAS  Google Scholar 

  47. P. WATTS, M. D. SMITH, I. EDWARDS, V. ZAMMIT, V. BROWN and H. GRANT, J. Hepatol. 23 (1995) 605

    Article  CAS  Google Scholar 

  48. I. GERBER and I. AP GWYNN, Eur. Cells Mater. 2 (2001) 10

    CAS  Google Scholar 

  49. M. WIEDMANN-AL-AHMAD, R. GUTWALD, G. LAUER, U. HÜBNER and R. SCHMELZEISEN, Biomaterials 23 (2002) 3319

    Article  CAS  Google Scholar 

  50. Y. ACIL, H. TERHEYDEN, A. DUNSCHE, B. FLEINER and S. JEPSEN, J. Biomed. Mater. Res. 51 (2000) 703

    Article  CAS  Google Scholar 

  51. F. GRIZON, E. AGUADO, G. HURE, M. F. BASLE and D. CHAPPARD, J. Dent. 30 (2002) 195

    Article  CAS  Google Scholar 

  52. M. CHRISTGAU, N. BADER, A. FELDEN, J. GRADL, A. WENZEL and G. SCHMALZ, J. Clin. Periodontol. 29 (2002) 710

    Article  CAS  Google Scholar 

  53. A.W. SUGAR, M. KURIAKOSE and N. D. WALSHAW, Int. J. Oral Maxillofac. Surg. 21 (1992) 140

    Article  CAS  Google Scholar 

  54. M. B. HÜRZELER, C. R. QUINONES, A. KIRSCH, C. GLOKER, P. SCHÜPACH, J. R. STRUB and R. G. CAFFESSE, Clin. Oral Impl. Res. 8 (1997) 467

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Klinik für Mund-, Kiefer- und Gesichtschirurgie, Universitätsklinikum Freiburg, Hugstetterstraße 55, Freiburg, 79106, Germany

    Sandra C. Schmitt, Margit Wiedmann-Al-Ahmad, Jens Kuschnierz, Ute Huebner, Rainer Schmelzeisen & Ralf Gutwald

  2. Universitätsklinik für Zahn-, Mund- und Kieferheilkunde, Abteilung für Zahnerhaltungskunde und Parodontologie, Albert-Ludwigs-Universität Freiburg, Hugstetterstraße 55, Freiburg, 79106, Germany

    Ali Al-Ahmad

Authors
  1. Sandra C. Schmitt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Margit Wiedmann-Al-Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jens Kuschnierz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ali Al-Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ute Huebner
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rainer Schmelzeisen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ralf Gutwald
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Margit Wiedmann-Al-Ahmad.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schmitt, S.C., Wiedmann-Al-Ahmad, M., Kuschnierz, J. et al. Comparative in vitro study of the proliferation and growth of ovine osteoblast-like cells on various alloplastic biomaterials manufactured for augmentation and reconstruction of tissue or bone defects. J Mater Sci: Mater Med 19, 1441–1450 (2008). https://doi.org/10.1007/s10856-007-3238-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3238-8

Keywords

Search

Navigation