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Behaviors of keratinocytes and fibroblasts on films of PLA50–PEO–PLA50 triblock copolymers with various PLA segment lengths

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Abstract

The growth of human primary keratinocytes and fibroblasts on PLA–PEO–PLA copolymer films was investigated as an intermediate stage of a strategy aimed at making implantable dermo-epidermal substitutes. Four PLA–PEO–PLA triblock copolymers with the same PEO block and different dl-lactic acid/ethylene oxide molar ratios (LA/EO) (0.8, 1.4, 1.8 and 2), were synthesized and characterized by 1H-nuclear magnetic resonance and infrared spectroscopy. The films made of these copolymers were more hydrophilic than PLA50 and than tissue culture polystyrene controls according to contact angles with water. Proliferation and adhesion of human skin cells were evaluated by MTT assay and by scanning electron microscopy. The presence of PEO in the triblock copolymers influenced cell adhesion and proliferation of fibroblasts, whereas keratinocyte adhesion and proliferation were not affected. These features emphasize the interest of PLA–PEO–PLA triblock copolymers to serve as better compounds than the racemic PLA previously investigated to make supports for human skin primary cells and scaffolds for skin engineering.

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Acknowledgements

The authors are grateful to Dr. Chantal Cazevieille (CRIC, University Montpellier I) for her technical assistance and interpreting data concerning ultrastructural evaluation, Sylvie Hunger for 1H NMR analysis and Dr. Andre Mas (UMR5073, University Montpellier II) for his help for the contact-angle measurements. This work was partly supported by the European Program INTELLISCAF (Intelligent Scaffolds for Tissue Engineering of Bone, Skin and Cartilage) (Contract No G5RD-CT-2002-00697).

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Authors and Affiliations

  1. Faculty of Pharmacy, CRBA, UMR CNRS 5473, University Montpellier 1, 15 Avenue Charles Flahault, Montpellier Cedex 5, 34093, France

    Xavier Garric, Henri Garreau & Michel Vert

  2. Laboratoire de Dermatologie Moléculaire UPRES EA 3754, IURC, University Montpellier I, 641 avenue du doyen G. Giraud, Montpellier Cedex 5, 34093, France

    Xavier Garric & Jean-Pierre Molès

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  1. Xavier Garric
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  2. Henri Garreau
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  4. Jean-Pierre Molès
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Correspondence to Xavier Garric.

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Garric, X., Garreau, H., Vert, M. et al. Behaviors of keratinocytes and fibroblasts on films of PLA50–PEO–PLA50 triblock copolymers with various PLA segment lengths. J Mater Sci: Mater Med 19, 1645–1651 (2008). https://doi.org/10.1007/s10856-007-3232-1

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