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Cultivation of human keratinocytes without feeder cells on polymer carriers containing ethoxyethyl methacrylate: in vitro study

Journal of Materials Science: Materials in Medicine volume 19, pages 883–888 (2008)Cite this article

Abstract

The cell/tissue engineering therapy of extensive or chronic skin wounds is a highly topical task of the contemporary medicine. One of possible therapeutic approaches is grafting of in vitro cultured keratinocytes directly to the wound bed, where the cells colonize the wound, proliferate and improve the re-epithelization process. Because the successful cultivation of keratinocytes needs an application of feeder cells, the exclusion of these cells from the cultivation system is highly required. In this study we show a positive influence of 2-ethoxyethyl methacrylate as a component of cultivation support on growth of keratinocytes without feeder cells. Keratinocytes cultured on these surfaces are able to migrate to the model wound bed in vitro, where they form distinct colonies and have a normal differentiation potential.

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Acknowledgements

This study was supported by grant grant No. 304/04/0171 of the Grant Agency of the Czech Republic and projects No. MSM0021620806 and No. 1M0021620803of the Ministry of Education, Youth and Sports of the Czech Republic. The authors are grateful to Mrs. Eva Vancová for excellent technical assistance.

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Affiliations

  1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Jiří Vacík, Jiří Michálek, Martin Přádný, Eva Krumbholcová & Taťána Fenclová

  2. 2nd Faculty of Medicine, Centre of Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic

    Jiří Vacík, Barbora Dvořánková, Jiří Michálek, Martin Přádný, Eva Krumbholcová & Karel Smetana Jr.

  3. 1st Faculty of Medicine, Institute of Anatomy, Charles University, U nemocnice 3, Prague 2, 128 00, Czech Republic

    Barbora Dvořánková & Karel Smetana Jr.

Authors
  1. Jiří Vacík
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  2. Barbora Dvořánková
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  3. Jiří Michálek
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  4. Martin Přádný
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  5. Eva Krumbholcová
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  6. Taťána Fenclová
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  7. Karel Smetana Jr.
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Correspondence to Karel Smetana Jr..

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Vacík, J., Dvořánková, B., Michálek, J. et al. Cultivation of human keratinocytes without feeder cells on polymer carriers containing ethoxyethyl methacrylate: in vitro study. J Mater Sci: Mater Med 19, 883–888 (2008). https://doi.org/10.1007/s10856-007-3225-0

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  • DOI: https://doi.org/10.1007/s10856-007-3225-0

Keywords

  • Czech Republic
  • HEMA
  • Feeder Cell
  • Polymer Carrier
  • HEMA Content