Skip to main content

Advertisement

SpringerLink
Log in

Osteopromotion with a plasmatransglutaminase on a β-TCP ceramic

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

We investigated the osteopromotive properties of plasmatransglutaminase (F XIII), bone marrow and venous blood on a resorbable β-tricalcium phosphate (β-TCP) scaffold. A baseline binding and release study of F XIII from the scaffold showed a continuous release of 18% of the total dose after 48 h. The main study consisted of 18 adult sheep with cylindrical defects in both tibiae. The defects were filled with a β-TCP cylinder impregnated either with bone marrow, venous blood, F XIII or sheep were treated with 1250 IU F XIII intravenously over 14 days (n = 4 in each group). The defects were left open in two sheep. QCT and histology was performed after 6 and 12 weeks. The best bone ingrowth was seen after 6 weeks in the bone marrow group and after 12 weeks in the local F XIII group. The highest ingrowth on the inside of the cylinder proving the osteopromoting potential of F XIII was found in the local F XIII group. In our opinion F XIII is a good and readily available osteopromoting agent which can be used with β-TCP in cases of bone deficit to promote bone regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. E. BECK, F. DUCKERT and M. ERNST, Thrombosis et diathesis haemorrhagica VI 3 (1961)

  2. T. NOLL and G. WOZNIAK, Hamostaseologie 22(1) (2002) 28

    Google Scholar 

  3. L. F. BROWN, N. LANIR, J. MCDONAGH, K. TOGNAZZI, A. M. DVORAK and H. F. DVORAK, AJP 142(1) (1993) 273

    CAS  Google Scholar 

  4. S. UEKI, J. TAKAGI and Y. SAITO, JCS 109 (1996) 2727

    CAS  Google Scholar 

  5. R. ERLEBACH, H. J. HARTUNG, In Klinische Aspekte des Faktor-XIII-Mangels, Diagnostik, klinische Relevanz, klinische Forschung, edited by R. EGBRING, R. SEITZ, G. WOZNIAK (Karger, Basel 1999) 89

    Google Scholar 

  6. Y. MISHIMA, F. NAGAO, K. ISHIBIKI, M. MATSUDA and N. NAKAMURA, Chirurg 55 (1984) 803

    CAS  Google Scholar 

  7. T. MUTO, Gelben Hefte 37 (1977) 83

    Google Scholar 

  8. S. J. BROCKMEIER, W. ARNOLD, In Klinische Aspekte des Faktor-XIII-Mangels, Diagnostik, klinische Relevanz, klinische Forschung, edited by R. EGBRING, R. SEITZ, G. WOZNIAK (Karger, Basel 1999) 147

    Google Scholar 

  9. S. BECKER, F. WEIDT and K. RÖHL, Spinal Cord 39 (2001) 114

    Article  CAS  Google Scholar 

  10. J. BENFER and H. STRUCK, Eur. Surg. Res. 9(3) (1977) 217

    CAS  Google Scholar 

  11. L. CLAES, C. BURRI, H. GERNGROSS and W. MUTSCHLER, Acta. Orthop. Scand. 56(1) (1985) 57

    Article  CAS  Google Scholar 

  12. H. GERNGROSS, A. STEINMANN and A. GRAF, Medwelt 38 (1987) 1533

    Google Scholar 

  13. R. SCHLENZKA, T. V. GARREL and C. PISTRO, JBJS [Br] 75B(Suppl II) (1993) 100

    Google Scholar 

  14. H. KIENAPFEL, R. SWAIN, A. HETTEL, A. WILKE, M. KOLLER and P. GRISS, Arch. Orthop. Trauma Surg. 116(4) (1997) 239

    Article  CAS  Google Scholar 

  15. R. E. MARX, Implant Dentistry 10 (2001) 4

    Google Scholar 

  16. J. F. CONNOLLY, Clin. Orthop. Rel. Res. 355 (1998) 257

    Google Scholar 

  17. Synthes GmbH, Surgical Technique “chronOS Perfusion Concept”, 2006, Oberdorf 036.000.745, (2006) 1

  18. Mathys Medical Ltd., Bone Graft Harvesting Set, Surgical Technique, 0336.188, (2002) 12

  19. Synthes GmbH, Bone Graft Harvesting Set, Surgical Technique, Oberdorf 0336.188, (2002) 12

  20. J. P. SCHMITZ and J. O. HOLLINGER, Clin. Orthop. Relat. Res. 205 (1986) 299

    Google Scholar 

  21. J. O. HOLLINGER and J. C. KLEINSCHMIDT, J. Craniofac. Surg. 1(1) (1990) 60

    Article  CAS  Google Scholar 

  22. P. S. EGGLI, W. MULLER and R. K. SCHENK, Clin. Orthop. Relat. Res. 232 (1988) 127

    CAS  Google Scholar 

  23. D. BUSER, B. HOFFMANN, J. P. BERNARD, A. LUSSI, D. METTLER and R. K. SCHENK, Clin. Oral Implants Res. 9(3) (1998) 137

    Article  CAS  Google Scholar 

  24. M. FINI, A. MOTTA, P. TORRICELLI, G. GIAVARESI, N. NICOLI ALDINI, M. TSCHON, R. GIARDINO and C. MIGLIARESI, Biomaterials 26(17) (2005) 3527

    Article  CAS  Google Scholar 

  25. R. K. SCHENK, In Guided bone regeneration in implant dentistry, edited by D. BUSER, C. DAHLIN, R. K. SCHENK (Quintessence Publishing Co., Inc., Chicago 1994) 49

    Google Scholar 

  26. J. SCHMID, B. WALLKAMM, C. H. HAMMERLE, S. GOGOLEWSKI and N. P. LANG, Clin. Oral Implants Res. 8(3) (1997) 244

    Article  CAS  Google Scholar 

  27. H. BURCHARDT and W. F. ENNEKING, Surg. Clin. North Am. 58(2) (1978) 403

    CAS  Google Scholar 

  28. H. BURCHARDT, Clin. Orthop. Relat. Res. 174 (1983) 28

    Google Scholar 

  29. K. D. JOHNSON, A. AUGUST, M. F. SCIADINI and C. SMITH, J. Orthop. Trauma 10(1) (1996) 28

    Article  CAS  Google Scholar 

  30. I. PONOMAREV, S. BECKER, T. STOLL, E. WRABETZ, M. ALINI and I. WILKE, Eur. Cell Mater. 5(2) (2003) 80

    Google Scholar 

  31. H. GERNGROSS, C. BURRI and L. CLAES, Arch. Orthop. Trauma Surg. 106(1) (1986) 23

    Article  CAS  Google Scholar 

  32. O. HELLERER, W. L. BRUCKNER, K. W. FREY, K. W. WESTERBURG and U. KLESSINGER, Arch. Orthop. Trauma Surg. 97(2) (1980) 157

    Article  CAS  Google Scholar 

  33. S. KACHIGAN, Statistical Analysis—An Interdisciplinary Introduction To Univariate and Multivariate Methods. (Radius Press, New York, 1986)

    Google Scholar 

  34. M. BOHNER, Injury 31(Suppl 4) (2000)

  35. D. TADIC and M. EPPLE, Biomaterials 25 (2004) 987

    Article  CAS  Google Scholar 

  36. P. S. EGGLI, W. MULLER and R. K. Schenk, Clin. Orthop. Relat. Res. 232 (1988) 127

    CAS  Google Scholar 

  37. T. STEFFEN, T. STOLL, T. ARVINTE and R. K. SCHENK, Eur. Spine J. 10 (2001) 132

    Article  Google Scholar 

Download references

Acknowledgements

The authors thank several people for their contribution and advice for this work:

C. Hackenbroich and C. Werner from the FZMB, Bad Langensalza, Germany, M. Bohner from the Dr. H. C. Robert Mathys Foundation, Bettlach, Switzerland, as well as L. Kaufman, Vrije Universiteit Brussel, Belgium for the statistical analysis. This study was performed with consent of the local animal rights committee and all animals utilized in their research were cared for according to the policies and principles established by the Animal Welfare Act and the NIH Guide for Care and Use of Laboratory Animals. The study was financed from a grant from ZLB Behring Research, Marburg, Germany and Synthes Biomaterials (Bettlach, Switzerland).

Author information

Authors and Affiliations

  1. Spine Center, Orthopedic Hospital Speising, Speisinger Str. 109, Vienna, 1134, Austria

    Stephan Becker

  2. AO Research Institute, Davos, 7270, Switzerland

    Otto Maissen, Thomas Meury, Christoph Sprecher & Mauro Alini

  3. Tissue Engineering Department, FZMB, Bad Langensalza, 99947, Germany

    Igor Ponomarev & Ingo Wilke

  4. Synthes Biomaterials, Oberdorf, 4436, Switzerland

    Thierry Stoll

Authors
  1. Stephan Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Otto Maissen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Igor Ponomarev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Thierry Stoll
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Thomas Meury
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Christoph Sprecher
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mauro Alini
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ingo Wilke
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stephan Becker.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Becker, S., Maissen, O., Ponomarev, I. et al. Osteopromotion with a plasmatransglutaminase on a β-TCP ceramic. J Mater Sci: Mater Med 19, 659–665 (2008). https://doi.org/10.1007/s10856-007-3223-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3223-2

Keywords

Search

Navigation