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Poly(HEMA) hydrogels with controlled pore architecture for tissue regeneration applications

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Abstract

The technique for fabrication of soft porous hydrogels, in which both the size and the orientation of inner pores can be controlled, was developed. Three-dimensional hydrophilic gels based on poly[2-hydroxyethyl methacrylate] are designed as scaffolds for regeneration of soft tissues, e.g., nerve tissue. Anisotropic macropores of the size ranging from 10 to 50 μm were formed (1) by using a porogen-leaching method with a solid organic porogen, (2) by phase-separation during gelation in solvent-nonsolvent mixture, or (3) by combination of solid porogen elimination and phase-separation. As a porogen, poly(l-lactide) fibers were applied and consequently washed away under mild conditions to obtain desired spatial orientation of pores. Highly water-swollen polymer gels were characterized with high pressure (low vacuum) scanning electron microscopy (AquaSEM). The morphology of voids remaining after removing the solid PLLA porogen (the macropores) was clearly shown.

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Acknowledgements

Support from the Grant Agency of the Czech Republic (Grant No. 203/04/P124), Ministry of Education of CR Research Centers Program (Grant No.: 1M 0538) and “EXPERTISSUES” EC 6th FP NMP-3 NoE No.: 500283-2 is acknowledged.

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  1. Department of Bioanalogous and Special Polymers, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic

    Hana Studenovská, Miroslav Šlouf & František Rypáček

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  1. Hana Studenovská
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  2. Miroslav Šlouf
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  3. František Rypáček
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Correspondence to Hana Studenovská.

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Studenovská, H., Šlouf, M. & Rypáček, F. Poly(HEMA) hydrogels with controlled pore architecture for tissue regeneration applications. J Mater Sci: Mater Med 19, 615–621 (2008). https://doi.org/10.1007/s10856-007-3217-0

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  • DOI: https://doi.org/10.1007/s10856-007-3217-0

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