Abstract
Chitosan (Chi) and poly (styrene sulfonate) (PSS) were employed to surface modify titanium thin film via electrostatic self-assembly (ESA) technique in order to improve its biocompatibility. The surface chemistry, wettability and surface topography of the coated films with different number of deposited layers were investigated by using X-ray photoelectron spectroscopy (XPS), water contact angle measurement and atomic force microscopy (AFM), respectively. The results indicated that a full surface coverage for the outmost layer was achieved at least after deposition of five layers, i.e., PEI/(PSS/Chi)2 on the titanium films. The formed multi-layered structure of PEI(PSS/Chi) x (x ≥ 2) on the titanium film was stable in air at room temperature and in phosphate buffered solution (PBS) for at least 3 weeks. Cell proliferation, cell viability, DNA synthesis as well as differentiation function (alkaline phosphatase) of osteoblasts on chitosan-modified titanium film (PEI/(PSS/Chi)6) and control sample were investigated, respectively. Osteoblasts cultured on chitosan-modified titanium film displayed a higher proliferation tendency than that of control (p < 0.01). Cell viability, alkaline phosphatase as well as DNA synthesis measurements indicated that osteoblasts on chitosan-modified titanium films were greater (p < 0.01) than those for the control, respectively. These results suggest that surface modification of titanium film was successfully achieved via deposition of PEI/(PSS/Chi) x layers, which is useful to enhance the biocompatibility of the titanium film.
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We gratefully acknowledge the financial support from the Natural Science Foundation of China (Grant 50603032), Natural Science Foundation Project of Chong Qing, CSTC (Grant 2007BA4004) and the partial financial support of the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Chong Qing University.
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Cai, K., Hu, Y., Jandt, K.D. et al. Surface modification of titanium thin film with chitosan via electrostatic self-assembly technique and its influence on osteoblast growth behavior. J Mater Sci: Mater Med 19, 499–506 (2008). https://doi.org/10.1007/s10856-007-3184-5
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DOI: https://doi.org/10.1007/s10856-007-3184-5