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Bone-like matrix formation on magnesium and magnesium alloys

Journal of Materials Science: Materials in Medicine volume 19, pages 407–415 (2008)Cite this article

Abstract

Mg metal and its alloys have promise as a biocompatible, degradable biomaterials. This work evaluates the potential of in vitro cell culture work with osteoblast-like cells on Mg based materials, and investigates cell differentiation and growth on Mg alloyed with various non-toxic or low-toxicity elements. Mg based substrates support the adhesion, differentiation and growth of stromal cells towards an osteoblast-like phenotype with the subsequent production of a bone like matrix under in vitro conditions. No significant difference in the final tissue layer is observed on pure Mg, an AZ21 alloy or a 0.5 wt% Ca alloy. Only a 0.8 wt% Ca alloy which shows complete structural disintegration shows minimal cell growth. Due to association of non-soluble degradation products formed when Mg is incubated in physiological-like fluid, mass changes typically used to report Mg degradation are not viable estimates of degradation. Methods quantifying the time dependent change in the mechanical integrity of samples as a function of incubation time are required for a proper assessment of Mg degradation. We conclude that in vitro cell culture of bone cells on Mg substrates is expected to be a viable screening technique to assess the relative biological activity of Mg-based materials.

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  1. http://en.wikipedia.org/wiki/Neodymium

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Acknowledgments

This work was financially supported by the Brian Mason Scientific & Technical Trust and University of Canterbury Grants U6513 and U6568. Thank you to Dr. David Aitchison for support. The authors also thank Mr. K. Stobbs, Mr. M. Flaws (Mechanical Engineering University of Canterbury) for technical assistance.

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Affiliations

  1. Department of Mechanical Engineering, University of Canterbury, Private Bag 4800, Christchurch, New Zealand

    Alexis Pietak & Mark P. Staiger

  2. Department of Anatomy & Structural Biology, University of Otago Medical School, P.O. Box 913, Dunedin, New Zealand

    Patricia Mahoney & George J. Dias

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  1. Alexis Pietak
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  2. Patricia Mahoney
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  3. George J. Dias
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  4. Mark P. Staiger
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Correspondence to Alexis Pietak.

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Pietak, A., Mahoney, P., Dias, G.J. et al. Bone-like matrix formation on magnesium and magnesium alloys. J Mater Sci: Mater Med 19, 407–415 (2008). https://doi.org/10.1007/s10856-007-3172-9

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  • DOI: https://doi.org/10.1007/s10856-007-3172-9

Keywords

  • Globular Cluster
  • Base Substrate
  • Osseous Tissue
  • Octacalcium Phosphate
  • Scratch Mark