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Bactericidal effects of bioactive glasses on clinically important aerobic bacteria

  • Eveliina Munukka
  • Outi Leppäranta
  • Mika Korkeamäki
  • Minna Vaahtio
  • Timo Peltola
  • Di Zhang
  • Leena Hupa
  • Heimo Ylänen
  • Jukka I. Salonen
  • Matti K. Viljanen
  • Erkki Eerola
Article

Abstract

Bioactive glasses (BAGs) have been studied for decades for clinical use, and they have found many dental and orthopedic applications. BAGs have also been shown to have an antibacterial effect e.g., on some oral microorganisms. In this extensive work we show that six powdered BAGs and two sol–gel derived materials have a clear antibacterial effect on 29 clinically important bacterial species. We also incorporated a rapid and accurate flow cytometric (FCM) method to calculate and standardize the numbers of viable bacteria inoculated in the suspensions used in the tests for antibacterial activity. In all materials tested growth inhibition could be demonstrated, although the concentration and time needed for the effect varied depending on the BAG. The most effective glass was S53P4, which had a clear growth-inhibitory effect on all pathogens tested. The sol–gel derived materials CaPSiO and CaPSiO II also showed a strong antibacterial effect. In summary, BAGs were found to clearly inhibit the growth of a wide selection of bacterial species causing e.g., infections on the surfaces of prostheses in the body after implantation.

Keywords

Propidium Iodide Bioactive Glass Neisseria Meningitidis Yersinia Enterocolitica Moraxella Catarrhalis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This study was supported by the National Technology Agency of Finland (TEKES).

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Eveliina Munukka
    • 1
    • 4
  • Outi Leppäranta
    • 1
  • Mika Korkeamäki
    • 1
    • 4
  • Minna Vaahtio
    • 2
    • 5
  • Timo Peltola
    • 2
    • 5
  • Di Zhang
    • 3
  • Leena Hupa
    • 3
  • Heimo Ylänen
    • 2
    • 3
  • Jukka I. Salonen
    • 2
  • Matti K. Viljanen
    • 1
  • Erkki Eerola
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of TurkuTurkuFinland
  2. 2.Turku Biomaterials CentreUniversity of TurkuTurkuFinland
  3. 3.Process Chemistry CentreÅbo Akademi UniversityTurkuFinland
  4. 4.CyFlo LtdTurkuFinland
  5. 5.Vivoxid Ltd.TurkuFinland

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