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Analysis of bovine-derived demineralized bone extracts

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Abstract

Demineralized bone factors are capable of stimulating bone regeneration through an osteoinductive mechanism and thus it has been recognized as a good bone graft. In this study, a kind of demineralized bone extracts (DBX) derived from bovine tibia by a chemical route. The extracts thus obtained were analyzed for their bio-chemical and physical properties using various techniques and results provided quite interesting insights into the demineralization process. There is no significant evidence of mineral phase associated with the connective tissue detected during chemical as well as physical testing, indicating the formation of DBX. This kind of bone extracts may used as a bone graft material and as a substrate for the growth of biomimetic apatites.

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Acknowledgement

The financial support of the National University of Singapore and the Singapore Millennium Foundation are gratefully acknowledged.

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Authors and Affiliations

  1. NUS Nanoscience and Nanotechnology Initiative (NUSNNI), Division of Bioengineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore, 117576, Singapore

    Ramlingam Murugan & Seeram Ramakrishna

  2. Spark Biotech, 3720 Sunburst Lane, Naperville, IL, 60564, USA

    Koritala Panduranga Rao

Authors
  1. Ramlingam Murugan
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  2. Seeram Ramakrishna
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  3. Koritala Panduranga Rao
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Correspondence to Ramlingam Murugan.

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Murugan, R., Ramakrishna, S. & Rao, K.P. Analysis of bovine-derived demineralized bone extracts. J Mater Sci: Mater Med 19, 2423–2426 (2008). https://doi.org/10.1007/s10856-007-3111-9

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  • DOI: https://doi.org/10.1007/s10856-007-3111-9

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