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Electrospun nanofibers immobilized with collagen for neural stem cells culture

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Abstract

Fibrous mats via electrospinning have been widely applied in tissue engineering. In this work, nanofibers were prepared via electrospinning from polymer with different content of carboxyl groups. A natural material, collagen, was then immobilized onto the nanofiber surface by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC)/N-Hydroxysuccinimide (NHS) activation process. It was found that the immobilization degree of collagen could be facilely modulated. The obtained collagen-modified nanofibers were used for neural stem cells culture, and unmodified nanofibers were used as a control. Results indicated that the modification of collagen could enhance the attachment and viability of the cultured neural stem cells.

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Acknowledgements

Financial support from the National Natural Science Foundation of China (Grant no. 30672152) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Neurosurgery, 3rd Affiliated Hospital of SUN YAT-SEN University, Guangzhou, 510630, P.R. China

    Wensheng Li, Ying Guo, Hui Wang, Dejin Shi, Chaofeng Liang, Zhuopeng Ye, Feng Qing & Jin Gong

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  1. Wensheng Li
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  2. Ying Guo
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  3. Hui Wang
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  4. Dejin Shi
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  5. Chaofeng Liang
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  6. Zhuopeng Ye
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  7. Feng Qing
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  8. Jin Gong
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Correspondence to Ying Guo.

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Li, W., Guo, Y., Wang, H. et al. Electrospun nanofibers immobilized with collagen for neural stem cells culture. J Mater Sci: Mater Med 19, 847–854 (2008). https://doi.org/10.1007/s10856-007-3087-5

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  • DOI: https://doi.org/10.1007/s10856-007-3087-5

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