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In vitro osteogenesis on fluorcanasite glass-ceramic with three different chemical compositions

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Abstract

This study aimed at investigating in vitro osteogenesis on three fluorcanasite glass-ceramic compositions with different solubilities (K3, K5, and K8). Osteoblastic cells were obtained from human alveolar bone fragments and cultured under standard osteogenic condition until subconfluence. First passage cells were cultured on K3, K5, and K8 and on Bioglass® 45S5 (45S5—control). Cell adhesion was evaluated at 24 h. For proliferation and viability, cells were cultured for 1, 4, and 10 days. Total protein content and alkaline phosphatase (ALP) activity were measured at 7, 14, and 21 days. Cultures were stained with Alizarin red at 21 days, for detection of mineralized matrix. Data were compared by ANOVA followed by Duncan’s test. Cell adhesion, cell proliferation, viability, total protein content, and ALP activity were not affected by fluorcanasite glass-ceramic composition and solubility. Bone-like formation was similar on all fluorcanasite-glass ceramics and was reduced compared to 45S5. The changes in the chemical composition and consequently solubility of the fluorcanasite glass-ceramics tested here did not significantly alter the in vitro osteogenesis. Further modifications of the chemical composition of the fluorcanasite glass-ceramic would be required to improve bone response, making this biomaterial a good candidate to be employed as a bone substitute.

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Acknowledgements

The authors would like to thank Ms. Júnia Ramos and Mr. Roger Rodrigo Fernandes for the technical assistance and their valuable co-operation in the experimental part of the present study. This work was supported by FAPESP (Grants # 02/13324-4 and 04/02130-0).

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Correspondence to Valdemar Mallet da Rocha Barros.

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Liporaci, J.L.J., Rosa, A.L., Beloti, M.M. et al. In vitro osteogenesis on fluorcanasite glass-ceramic with three different chemical compositions. J Mater Sci: Mater Med 19, 833–838 (2008). https://doi.org/10.1007/s10856-007-3081-y

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  • DOI: https://doi.org/10.1007/s10856-007-3081-y

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