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Immune evaluation of biomaterials in TNF-α and IL-1β at mRNA level

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Abstract

Objective

To examine biomaterial biocompatibility and improve current methods of immunological evaluation TNF-α and IL1-β were used as indicators at mRNA levels.

Methods

Rat peritoneal macrophages were stimulated with different biomaterials and expression of TNF-α and IL1-β was measured by RT-PCR and compared within different groups.

Results

Macrophages that were stimulated with PTFE produced significantly more TNF-α and IL1-β than unstimulated macrophages (p < 0.001). The PLGA, NPG, β-TCP, and CPC groups also induced moderate TNF-α and IL1-β expression (p < 0.01).

Conclusions

TNF-α and IL1-β are sensitive indicators of immune stimulation that can help to monitor the levels of cellular activation induced by different biomaterials. Our findings showed that β-TCP and CPC had a good biocompability, and CPC was the most biocompatible of all the biomaterials tested. While PTFE and NPG still had side effect because of producing pro-inflammatory cytokines in vitro once implanted.

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Acknowledgements

This research was partially supported by the Shanghai Biomaterials Research and Testing Center and the School of Life Science, East China Normal University, Shanghai. The authors acknowledge Prof. Jiao Sun and Associate Prof. Ping Zhang for their assistance in the research. Supported by grants from National Natural Science Foundation of China (30470479), Shanghai Leading Academic Discipline found (T0202), and Shanghai Sci-Tech Committee Foundation (0452nm043).

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Authors and Affiliations

  1. Ninth People’s Hospital School of Medicine, Shanghai Jiao tong University/Shanghai Biomaterials Research & Testing Center, Shanghai, 200023, China

    Tingting Ding & Jiao Sun

  2. School of Life Science, East China Normal University, Shanghai, 200062, China

    Ping Zhang

Authors
  1. Tingting Ding
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  2. Jiao Sun
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  3. Ping Zhang
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Correspondence to Jiao Sun.

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Ding, T., Sun, J. & Zhang, P. Immune evaluation of biomaterials in TNF-α and IL-1β at mRNA level. J Mater Sci: Mater Med 18, 2233–2236 (2007). https://doi.org/10.1007/s10856-007-3014-9

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  • DOI: https://doi.org/10.1007/s10856-007-3014-9

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