Hydroxyapatite containing superporous hydrogel composites: synthesis and in-vitro characterization

Abstract

The synthesis of an acrylamide-based superporous hydrogel composite (SPHC) with hydroxyapatite (HA) was realized by solution polymerization technique. The characterization studies were performed by FTIR studies, determination of swelling kinetics, measurement of mechanical properties, SEM/EDAX studies and cytocompatibility tests. The FTIR and EDAX studies revealed the incorporation of HA in superporous hydrogel (SPH) structure. The results obtained from swelling experiments showed that, although the extent of swelling was decreased after incorporation of HA in SPH structure, the time to reach the equilibrium swelling was not affected for SPHC. This result indicated that, the presence of HA did not block the capillary channels and the interconnected pore structure was maintained which were consistent with the images obtained from SEM photographs. The results obtained from mechanical tests showed that, in the presence of HA, the compression strength of the hydrogel composite was improved significantly when compared to SPH structure. The compressive modulus for the SPHC increased to 6.59 ± 0.35 N/mm² whereas it was 0.63 ± 0.04 N/mm² for the SPH. The cytocompatibility test which was performed by using L929 fibroblasts showed that both the SPH and SPHC materials were cytocompatible towards fibroblasts. The synthesized superporous hydrogel composite possesses suitable properties especially for bone tissue engineering applications and shall be considered as a novel scaffold.