Abstract
Taking into consideration that DNA damage plays an important role in carcinogenesis, the purpose of this study was to evaluate whether some radiopacifiers widely used in clinical practice are able to induce genetic damage in primary human cells in vitro. Human peripheral lymphocytes obtained from 10 healthy volunteers were exposed to barium sulphate (BaSO4), zirconium oxide (ZnO2) and bismuth oxide (Bi2O3) at final concentrations ranging from 1 to 1000 μg/mL for 1 h at 37 °C. The negative control group was treated with vehicle control (phosphate buffer solution) for 1 h at 37 °C and the positive control group was treated with hydrogen peroxide (at 100 μM) for 5 min on ice. Results were analyzed by the Friedman non-parametric test. The results pointed all compounds tested out did not induce DNA breakage in human peripheral lymphocytes as depicted by the mean tail moment and tail intensity in all concentrations tested. In summary, our results indicate that exposure to these radiopacifiers may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single cell gel (comet) assay.
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Acknowledgements
We are thankful to Maria Luiza Falaguera Ardanaz for her technical assistance. This work was supported by grants from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo).
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Braz, M.G., de Castro Marcondes, J.P., Matsumoto, M.A. et al. Genotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitro. J Mater Sci: Mater Med 19, 601–605 (2008). https://doi.org/10.1007/s10856-007-3000-2
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DOI: https://doi.org/10.1007/s10856-007-3000-2