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Gelation under dynamic conditions: A strategy for in vitro cell ordering

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Abstract

Ordered gelation under spin-coating conditions, as reported here, is a suitable method to order cells in biogels. Cell ordering is of great importance for functional repair of central nervous system (CNS) injuries, because therapies must include strategies to bridge chystic gaps and facilitate axon growth towards its target. Organized biocompatible and biodegradable substrates may be used for this purpose, to supply trophic support and provide directional cues for neuronal process outgrowth. Atomic force microscopy (AFM) and low temperature scanning electron microscopy (LTSEM), confirmed that fibrils in κ–carrageenan/chitosan and fibrin hydrogels prepared under spin-coating conditions, were longitudinally arranged. The cell model was conveniently tested using rat C6 glioma cells. C6 cells were distributed regularly in fibrin gels formed under centrifugal force. The ability of ordered fibrin scaffolds to promote uniform distribution of transplanted cells, was confirmed by fluorescence microscopy.

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Authors and Affiliations

  1. Unidad de Neurología Experimental, CSIC, SESCAM, Hospital Nacional de Parapléjicos, Finca “La Peraleda” s/n, 45071, Toledo, Spain

    Ernesto Doncel-Pérez & Manuel Nieto-Sampedro

  2. Departamento de Plasticidad Neural, Instituto Cajal, CSIC, Doctor Arce 37, 28002, Madrid, Spain

    Eduardo Martín-López & Manuel Nieto-Sampedro

  3. Instituto de Ciencia de Materiales de Madrid, CSIC, Cantoblanco, 28049, Madrid, Spain

    Margarita Darder, Luis Vázquez & Eduardo Ruiz-Hitzky

Authors
  1. Ernesto Doncel-Pérez
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  2. Margarita Darder
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  3. Eduardo Martín-López
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  4. Luis Vázquez
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  5. Manuel Nieto-Sampedro
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  6. Eduardo Ruiz-Hitzky
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Correspondence to Ernesto Doncel-Pérez.

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Doncel-Pérez, E., Darder, M., Martín-López, E. et al. Gelation under dynamic conditions: A strategy for in vitro cell ordering. J Mater Sci: Mater Med 17, 795–802 (2006). https://doi.org/10.1007/s10856-006-9837-y

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  • DOI: https://doi.org/10.1007/s10856-006-9837-y

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