Skip to main content
SpringerLink
Log in

The effects of types of degradable polymers on porcine chondrocyte adhesion, proliferation and gene expression

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Understanding how a specific biomaterial may influence chondrocyte adhesion, proliferation and gene expression is important in cartilage tissue engineering. In this study several biodegradable polymers that are commonly used in tissue engineering were evaluated with respect to their influence on chondrocyte attachment, proliferation and gene expression. Primary cultures of porcine chondrocytes were performed in films made of poly-L-lactic acid (PLLA), poly-D,L-lactic acid (PDLLA), poly-(lactide-co-glycolide) (PLGA), or polycaprolactone (PCL). Chondrocytes adhered to PDLLA or PLGA after 1-day incubation better than to PLLA or PCL. After 7 or 14 day culture, the cell numbers on PDLLA or PLGA was still higher than PLLA or PCL. The results suggested that cell attachment and growth might depend on degradation rate of biodegradable polymers. Along with the fact that PDLLA or PLGA supported expression of chondrocyte specific genes more than PLLA or PCL, the former two materials seemed to be more suitable for cartilage tissue engineering than the latter ones. Besides, we found that chondrocyte phenotype prior to seeding was important in the expression of ECM proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. I. CAPLAN, Sci Am 251 (1984) 84.

    CAS  Google Scholar 

  2. A. I. CAPLAN, M. ELYADERANI, Y. MOCHIZUKI, S. WAKITANI and V. M. GOLDBERG, Clin. Orthop. (1997) 254.

  3. S. W. O’DRISCOLL, F. W. KEELEY and R. B. SALTER, J. Bone. Joint. Surg. Am. 68 (1986) 1017.

    CAS  Google Scholar 

  4. E. BELL, in “Principles of Tissue Engineering” (Academic Press, USA, 2000). p. xxxv.

  5. J. A. HUBBELL, Bio-Technology 13 (1995) 565.

    CAS  Google Scholar 

  6. L. E. FREED, G. Vunjak-Novakovic, R. J. Biron, D. B. Eagles, D. C. Lesnoy and S. K. BARLOW, et al., Biotechnology (N Y) 12 (1994) 689.

    Article  CAS  Google Scholar 

  7. L. G. CIMA, J. P. VACANTI, C. VACANTI, D. INGBER, D. MOONEY and R. LANGER, J. Biomech. Eng. 113 (1991) 143.

    CAS  Google Scholar 

  8. L. E. FREED, J. C. MARQUIS, A. NOHRIA, J. EMMANUAL, A. G. MIKOS and R. LANGER, J. Biomed. Mater. Res. 27 (1993) 11.

    Article  CAS  Google Scholar 

  9. N. A. PEPPAS and R. LANGER, Science 263 (1994) 1715.

    CAS  Google Scholar 

  10. S. PULAPURA and J. KOHN, J. Biomater. Appl. 6 (1992) 216.

    CAS  Google Scholar 

  11. D. A. GRANDE, C. HALBERSTADT, G. NAUGHTON, R. SCHWARTZ and R. MANJI, J. Biomed. Mater. Res. 34 (1997) 211.

    Article  CAS  Google Scholar 

  12. M. SITTINGER, D. Reitzel, M. Dauner, H. Hierlemann, C. Hammer and E. KASTENBAUER, et al., J. Biomed. Mater. Res. 33 (1996) 57.

    Article  CAS  Google Scholar 

  13. S. L. ISHAUG-RILEY, L. E. OKUN, G. PRADO, M. A. APPLEGATE and A. RATCLIFFE, Biomaterials 20 (1999) 2245.

    Article  CAS  Google Scholar 

  14. S. H. HSU, C. L. TSAI and C. M. TANG, Artif. Organs 26 (2002) 647.

    Article  CAS  Google Scholar 

  15. K. VON DER MARK, V. GAUSS, H. VON DER MARK and P. MULLER, Nature 267 (1977) 531.

    Article  CAS  Google Scholar 

  16. P. BENYA, J. SCHAFFER, Cell 30 (1982) 215.

    Article  CAS  Google Scholar 

  17. K. ELIMA, E. VUORIO, FEBS Lett. 258 (1989) 195.

    Article  CAS  Google Scholar 

  18. V. LEFEBVRE, C. PEETERS-JORIS and G. VAES, Biochim. Biophys. Acta. 1051 (1990) 266.

    CAS  Google Scholar 

  19. C. W. ARCHER, J. MCDOWELL, M. T. BAYLISS, M. D. STEPHENS and G. BENTLEY, J. Cell. Sci. 97 (Pt 2) (1990) 361.

    Google Scholar 

  20. A. L. AULTHOUSE, M. BECK, E. GRIFFEY, J. SANFORD, K. ARDEN and M. A. MACHADO, et al., In Vitro Cell Dev. Biol. 25 (1989) 659.

    CAS  Google Scholar 

  21. Y. J. KIM, R. L. SAH, J. Y. DOONG and A. J. GRODZINSKY, Anal. Biochem. 174 (1988) 168.

    Article  CAS  Google Scholar 

  22. P. CHOMCZYNSKI and N. SACCHI, Anal. Biochem. 162 (1987) 156.

    Article  CAS  Google Scholar 

  23. T. A. HORBETT and M. B. SCHWAY, J. Biomed. Mater. Res. 22 (1988) 763.

    CAS  Google Scholar 

  24. M. D. EVANS and J. G. STEELE, J. Biomed. Mater. Res. 40 (1998) 621.

    Article  CAS  Google Scholar 

  25. S. I. ERTEL, B. D. RATNER and T. A. HORBETT, J. Biomed. Mater. Res. 24 (1990) 1637.

    Article  CAS  Google Scholar 

  26. T. A. HORBETT, J. J. WALDBURGER, B. D. RATNER and A. S. HOFFMAN, J. Biomed. Mater. Res. 22 (1988) 383.

    CAS  Google Scholar 

  27. W.-B. TSAI, T.-C. WEI, M.-C. LIN, J.-Y. WANG and C.-H. CHENM, J. Biomater. Sci. Polym. Edn. 16 (2005) 699.

    CAS  Google Scholar 

  28. C. G. PITT, T. A. Marks and A. SCHINDLER, in “Controlled Release of Bioactive Materials” (Academic Press, Inc., New York, 1980). p. 19.

    Google Scholar 

  29. S. KAY, A. THAPA, K. M. HABERSTROH and T. J. WEBSTER, Tissue Eng. 8 (2002) 753.

    Article  CAS  Google Scholar 

  30. J. GAO, L. NIKLASON and R. LANGER, J. Biomed. Mater Res. 42 (1998) 417.

    Article  CAS  Google Scholar 

  31. V. SALDANHA and D. A. GRANDE, Biomaterials 21 (2000) 2427.

    Article  CAS  Google Scholar 

  32. B. DE CROMBRUGGHE, V. LEFEBVRE, R. R. BEHRINGER, W. BI, S. MURAKAMI and W. HUANG, Matrix Biol. 19 (2000) 389.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei, 106, Taiwan

    Wei-Bor Tsai & Chun-Hong Chen

  2. ITRI, Biomedical Engineering Center, Hsinchu, 300, Taiwan

    Jing-Fu Chen & Ken-Yuan Chang

Authors
  1. Wei-Bor Tsai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chun-Hong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jing-Fu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ken-Yuan Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei-Bor Tsai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsai, WB., Chen, CH., Chen, JF. et al. The effects of types of degradable polymers on porcine chondrocyte adhesion, proliferation and gene expression. J Mater Sci: Mater Med 17, 337–343 (2006). https://doi.org/10.1007/s10856-006-8234-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-8234-x

Keywords

Search

Navigation