A folic acid-based functionalized surface for biosensor systems

  • Kaustubh D. BhaleraoEmail author
  • Stephen C. Lee
  • Winston O. Soboyejo
  • Alfred B. O. Soboyejo


The performance of a biosensor depends largely on its interface with the biological system. This interface imparts a biologically relevant function to the device and provides a measure of specificity towards the biological analyte of interest. This paper documents the choice of folic acid as the functional component of a cantilever sensor to recognize nasopharyngeal (KB) cancer cells. A conjugation chemistry protocol has been outlined to deploy folic acid onto a titanium-coated sensor surface using a silane linker. The presence and biological activity of the sensor was verified by means of an immmunospecific (ELISA) procedure. The overall performance of the folic acid-based cantilever sensor was measured using cancerous KB cell-binding experiments.


Folic Acid Titanium Surface Folate Receptor Micro Electro Mechanical System Reduce Folate Carrier 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Kaustubh D. Bhalerao
    • 1
    Email author
  • Stephen C. Lee
    • 2
  • Winston O. Soboyejo
    • 3
  • Alfred B. O. Soboyejo
    • 4
  1. 1.Department of Agricultural and Biological EngineeringUniversity of Illinois at Urbana-ChampaignUrbana
  2. 2.Department of Biomedical Engineering, Department of Chemical Engineering and The Davis Heart & Lung InstituteThe Ohio State UniversityColumbus
  3. 3.Department of Mechanical and Aerospace Engineering and The Princeton Materials InstitutePrinceton UniversityPrinceton
  4. 4.Department of Food, Agricultural and Biological Engineering, Department of Aerospace Engineering and The John Glenn Institute of Public Policy and Public ServiceThe Ohio State UniversityColumbus

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