Skip to main content

Cytotoxicity study of plasma-sprayed hydroxyapatite coating on high nitrogen austenitic stainless steels

Journal of Materials Science: Materials in Medicine volume 17, pages 1095–1100 (2006)Cite this article

Abstract

Stainless steel has been frequently used for temporary implants but its use as permanent implants is restricted due to its low pitting corrosion resistance. Nitrogen additions to these steels improve both mechanical properties and corrosion resistance, particularly the pitting and crevice corrosion resistance. Many reports concerning allergic reactions caused by nickel led to the development of nickel free stainless steel; it has excellent mechanical properties and very high corrosion resistance. On the other hand, stainless steels are biologically tolerated and no chemical bonds are formed between the steel and the bone tissue. Hydroxyapatite coatings deposited on stainless steels improve osseointegration, due their capacity to form chemical bonds (bioactive fixation) with the bone tissue. In this work hydroxyapatite coatings were plasma-sprayed on three austenitic stainless steels: ASTM-F138, ASTM-F1586 and the nickel-free Böhler-P558. The coatings were analyzed by SEM and XDR. The cytotoxicity of the coatings/steels was studied using the neutral red uptake method by quantitative evaluation of cell viability. The three uncoated stainless steels and the hydroxyapatite coated Böhler-P558 did not have any toxic effect on the cell culture. The hydroxyapatite coated ASTM-F138 and ASTM-F1586 stainless steels presented cytotoxicity indexes (IC50%) lower than 50% and high nickel contents in the extracts.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. D. WILLIAMS, in “Fundamental aspects of biocompatibility” (CRC Press, Boca Raton, 1981) Vol. II, p. 307.

  2. K. MERRIT and S. BROWN, Clin. Orthop. Rel. Res. 1-329S (1996) S233.

    Article  Google Scholar 

  3. D. J. GAWKRODGER, Contact Dermatitis. 28 (1993) 257.

    CAS  Article  Google Scholar 

  4. M. B. MAYOR, K. MERRIT and S. BROWN, Am. J. Surg. 139 (1980) 477.

    CAS  Article  Google Scholar 

  5. L. KANERVA and L. FÖRSTRÖM, Contact Dermatitis. 44 (2001) 103.

    CAS  Article  Google Scholar 

  6. P. HAUDRECHY, J. FOUSSEREAU, B. MANTOUT and B. BAROUX, Contact Dermatitis. 31 (1994) 249.

    CAS  Article  Google Scholar 

  7. V. G. GAVRILJUK and H. BERNS, “High Nitrogen Steels. Structure, proprieties, manufacture, applications” (Springer, Germany, 1999) p. 377.

    Google Scholar 

  8. W. C. JR. DA SILVA, C. H. AIDAR, F. PANONI and A. P. TSCHIPTSCHIN, in Stainless Steel World 1999 Conference, The Hague Nederland’s, Conference papers—Book 2 (1999) p. 763.

  9. P. J. UGGOWITZER, R. MAGDOWSKI and M.O. SPEIDEL, ISIJ Int. 36 (1996) 901.

    CAS  Google Scholar 

  10. L. L. HENCH and J. WILSON, “An introduction to bioceramics” (World Scientific, Singapore, 1993) p. 386.

    Google Scholar 

  11. G. CIAPETTI, D. GRANCHI, E. VERRI, L. SAVARINO, D. CAVEDAGNA and A. PIZZOFERRATO, Biomaterials 17 (1996) 1259.

    CAS  Article  Google Scholar 

  12. S. O. ROGERO, A. B. LUGÃO, T. I. IKEDA and A. S. CRUZ, Mater. Res. 6-3 (2003) 317.

    Google Scholar 

  13. B. KOCH, J. G. C. WOLKE and K. DE GROOT, J. Biomed. Mater. Res. 24 (1990) 655.

    CAS  Article  Google Scholar 

  14. S. KOUTSOPOULOS, J. Biomed. Mater. Res. 62 (2002) 600.

    CAS  Article  Google Scholar 

  15. R. McPHERSON, N. GANE and T. J. BASTOW, J. Mater. Sci.-Mater. Med. 6 (1995) 327.

    CAS  Article  Google Scholar 

  16. L. SUN, C. C. BERNDT, K. A. GROSS and A. KUCUK, J. Biomed. Mater. Res. (Appl. Biomater.) 56 (2001) 592.

    Google Scholar 

  17. K. BORDJI, J. JOUSEAU, D. MAINARD, E. PAYAN, J. P. DELAGOUTTE and P. NETTER, Biomaterials 17 (1996) 491.

    CAS  Article  Google Scholar 

  18. I. C. LAVOS-VARELETO, I. COSTA and S. WOLYNEC, J. Biomed. Mater. Res. (Appl. Biomater.) 63 (2002) 664.

    Article  Google Scholar 

  19. K. T. OH and Y. S. PARK, Surf. Coat. Tech. 110 (1998) 4.

    CAS  Article  Google Scholar 

  20. C. P. O. OSSA and A. P. TSCHIPTSCHIN, Proceedings of High Nitrogen Steels Ostend, Belgium, September (2004), Steel Grips No. 2 Suppl. p. 667.

Download references

Author information

Affiliations

  1. Bioengineering Program, University of Antioquia, Calle 67 N°53-108, Of. 18-133, A.A.1226, Medellín, Colombia

    C. P. O. Ossa

  2. Metallurgical and Materials Engineering Department, University of São Paulo, Avenida Professor Mello Moraes 2463, 05508-900, São Paulo, Brazil

    A. P. Tschiptschin

  3. Nuclear and Energy Research Institute, IPEN, Avenida Lineu Prestes 2242, 05508-000, São Paulo, Brazil

    S. O. Rogero

Authors
  1. C. P. O. Ossa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. O. Rogero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. P. Tschiptschin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. P. O. Ossa.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ossa, C.P.O., Rogero, S.O. & Tschiptschin, A.P. Cytotoxicity study of plasma-sprayed hydroxyapatite coating on high nitrogen austenitic stainless steels. J Mater Sci: Mater Med 17, 1095–1100 (2006). https://doi.org/10.1007/s10856-006-0536-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-0536-5

Keywords

  • Hydroxyapatite
  • Austenitic Stainless Steel
  • P558 Steel
  • Calcium Oxide
  • Tricalcium Phosphate