Skip to main content

Advertisement

SpringerLink
Log in

Implants of polyanionic collagen matrix in bone defects of ovariectomized rats

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

In recent years, there has been a great interest in the development of biomaterials that could be used in the repair of bone defects. Collagen matrix (CM) has the advantage that it can be modified chemically to improve its mechanical properties. The aim of the present study was to evaluate the effect of three-dimensional membranes of native or anionic (submitted to alkaline treatment for 48 or 96 h) collagen matrix on the consolidation of osteoporosis bone fractures resulting from the gonadal hormone alterations caused by ovariectomy in rats subjected to hormone replacement therapy. The animals received the implants 4 months after ovariectomy and were sacrificed 8 weeks after implantation of the membranes into 4-mm wide bone defects created in the distal third of the femur with a surgical bur. Macroscopic analysis revealed the absence of pathological alterations in the implanted areas, suggesting that the material was biocompatible. Microscopic analysis showed a lower amount of bone ingrowth in the areas receiving the native membrane compared to the bone defects filled with the anionic membranes. In ovariectomized animals receiving anionic membranes, a delay in bone regeneration was observed mainly in animals not subjected to hormone replacement therapy. We conclude that anionic membranes treated with alkaline solution for 48 and 96 h presented better results in terms of bone ingrowth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. S. L. ISHAUG-RILEY, G. M. CRANE and A. GURLEK, J. Biomed. Mater. Res. 36 (1997) 1

    Article  CAS  Google Scholar 

  2. D. E. MARK, J. O. HOLLINGER, J. R. HASTINGS, G. CHEN, L. J. MARDEN and A. H. REDDI, Plast. Reconstr. Surg. 86 (1990) 623

    CAS  Google Scholar 

  3. E. M. YOUNGER and M. N. CHAPMAN, J. Orthop. Trauma 3 (1989) 192

    Article  CAS  Google Scholar 

  4. A. S. BREITBART, D. A. GRANDE, R. KESSLER, J. T. JAMESRYABY, R. J. FITZSIMMONS and R. T. GRANT, Plast. Reconstr. Surg. 101 (1998) 567

    Article  CAS  Google Scholar 

  5. R. E WEISS and A. H. REDDY, J. Cell Biol. 88 (1981) 630

    Article  CAS  Google Scholar 

  6. B. S. KIM and D. J. MOONEY, Trends Biotechnol. 16 (1998) 224

    Article  CAS  Google Scholar 

  7. C. YANG, P. J. HILLAS, J. A. BAEZ, M. NOKELAINEN, J. BALAN, J. TANG, R. SPIRO and J. W. POLAREK, Biodrugs 18 (2004) 103

    Article  CAS  Google Scholar 

  8. Y. S. CHEN, C. L. HSIEH, C. C. TSAI, T. H. CEHEN, W. C. CHENG, C. L. HU and C. H. YAO, Biomaterials 21 (2000) 1541

    Article  CAS  Google Scholar 

  9. F. GRINNELL, Trends Cell Biol. 13 (2003) 264

    Article  CAS  Google Scholar 

  10. A. R. J. SANTOS, H. DOLDER and M. L. F. WADA, J. Submicrosc. Cytol. Pathol. 35 (2003) 35

    Google Scholar 

  11. G. G. REID, S. D. GORHAM and J. M. LACKIE, J. Mater. Sci.: Mater. Med. 4 (1993) 201

    Article  CAS  Google Scholar 

  12. M. R. CUNHA, A. R. SANTOS Jr and S. C. GENARI, Bol. Med. Vet. 1(2005) 73

    Google Scholar 

  13. M. P. SINGH, J. STEFKO, J. A. LUMPKIN and J. ROSENBLATT, Pharm. Res. 12 (1995) 1205

    Article  CAS  Google Scholar 

  14. T. L. TUAN, L. C. KELLER, D. SUN, M. E. NIMNI and D. CHEUNG, J. Cell Sci. 107 (1994) 285

    Google Scholar 

  15. A. M. D. PELPIS, G. GOISSIS and D. K. DAS-GUPTA, Polym. Eng. Sci. 36 (1996) 2932

    Article  Google Scholar 

  16. G. GOISSIS, C. LACERDA, M. P. BARBOSAS and A. PINATTI, Rev. Bras. Engenharia Bioméd. 15 (1999) 55

    Google Scholar 

  17. M. R. BET, G. GOISSIS and C. A. LACERDA, Biomacromolecules 2 (2001) 1074

    Article  CAS  Google Scholar 

  18. L. B. ROCHA, G. GOISSIS and M. A. ROSSI, Biomaterials 23 (2002) 449

    Article  CAS  Google Scholar 

  19. F. P. ROSA, R. C. C. LIA, K. O. F. SOUZA, G. GOISSIS and J. E. MARCANTONIO, Biomaterials 24 (2003) 207

    Article  CAS  Google Scholar 

  20. P. FRAYSSINET, Biomaterials 13 (1992) 668

    Article  CAS  Google Scholar 

  21. V. SHEN, R. BIRCHMAN, D. D. WU and R. LINDSAY, J. Bone Miner. Res. 15 (2000) 740

    Article  CAS  Google Scholar 

  22. G. A. LINCOLN and N. J. TYLER, J. Reprod. Fertil. 115 (1999) 167

    CAS  Google Scholar 

  23. T. J. WRONSKI, L. M. DANN, K. S. SCOTT and M. CINTRON, Calcif. Tissue Int. 45 (1989) 360

    Article  CAS  Google Scholar 

  24. C. A. MANDARIM DE LACERDA, Braz. J. Morphol. 16 (1999) 131

    Google Scholar 

  25. C. Y. KRAMMER, Biometrics 12 (1956) 307

    Article  Google Scholar 

  26. Y. C. JUNG, C. H. HAN, I. S. LEE and H. E. KIM, Int. J. Oral Maxillofac. Implants 16 (2001) 809

    CAS  Google Scholar 

  27. T. OZAWA, K. IIDA, C. SUKOTJO, H. HASEGAWA, R. D. NISHIMURA and I. NISHIMURA, Bone 30 (2002) 137

    Article  CAS  Google Scholar 

  28. J. LI, E. Y. LIAO, R. C. DAÍ, Q. Y. WEI and X. H. LUO, J. Mol. Histol. 35 (2004) 723

    Article  CAS  Google Scholar 

  29. G. GIAVARESI, V. BORSARI, M. FINI, L. MARTINI, M. TSCHON, F. DETERLIZZI, A. NICOLINI, A. CARPI and R. GIARDINO, Biomed. Pharmacother. 58 (2004) 494

    Article  CAS  Google Scholar 

  30. M. MUKHERJEE, A. S. DAS, S. MITRA and C. MITRA, Phytother. Res. 18 (2004) 389

    Article  CAS  Google Scholar 

  31. P. CHO, G. B. SCHNEIDER, K. KRIZAN and J. C. KELLER, Implant Dent. 13 (2004) 79

    Article  Google Scholar 

  32. J. W. WANG, W. LI, S. W. XU, D. S. YANG, Y. WANG, M. LIN and G. F. ZHAO, Chin. J. Traumatol. 8 (2005) 111

    Google Scholar 

  33. H. FURUE, M. YOSHIMURA and A. ITO, Clin. Calcium 15 (2005) 163

    Google Scholar 

  34. M. KRUKOWSKI, in “Biomaterial Interface” (Toronto, University of Toronto Press, 1991) p. 275

  35. J. E. DAVIES, B. CAUSTON, Y. BOVELL, K. DAVY and C. S. STURT, Biomaterials 7 (1986) 231

    Article  CAS  Google Scholar 

  36. S. HSU and W. C. CHEN, Biomaterial 2 (2000) 359

    Article  Google Scholar 

  37. M. R. BET, G. GOISSIS, S. VARGAS and H. S. SELISTRE-DE-ARAUJO, Biomaterials 24 (2003) 131

    Article  CAS  Google Scholar 

  38. G. GOISSIS, V. S. MAGINADOR and V. C. A. MARTINS, Artif. Organs 27 (2003) 437

    Article  CAS  Google Scholar 

  39. P. L. MOREIRA, Y. H. AN, J. A. R. SANTOS and S. C. GENARI, J. Biomed. Mater. Res. 71 (2004) 229

    Article  CAS  Google Scholar 

  40. P. B. SAADEH, R. K. KHOSLA, B. J. MEHRARA, D. S. STEINBRECH, S. A. MCCORMICK, D. P. DEVORE and M. T. LONGAKER, J. Craniofac. Surg. 12 (2001) 573

    Article  CAS  Google Scholar 

  41. T. ALBREKTSSON, P. I. BRANEMARK, H. A. HANSSON and J. LINDSTROM, Acta Orthop. Scand. 52 (1981) 155

    Article  CAS  Google Scholar 

  42. J. A. CAMILLI, M. R. CUNHA, C. A. BERTRAN and E. Y. KAWACHI, Arch. Oral Biol. 49 (2004) 747

    Article  CAS  Google Scholar 

  43. J. B. CÉSAR NETO, P. M. DUARTE, E. A. SALLUM, D. BARBIERI, H. MORENO and F. H. NOCITI, J. Periodontol. 74 (2003) 1454

    Article  Google Scholar 

  44. F. H. NOCITI, C. M. STEFANI, E. A. SALLUM, P. M. DUARTE and A. W. SALLUM, Implant Dent. 11 (2002) 176

    Article  Google Scholar 

  45. W. ZECHNER, N. TRINKL, G. WATZAK, D. BUSENLECHNER, G. TEPPER, R. HAAS and G. WATZEK, Int. J. Oral Maxillofac. Implants 19 (2004) 216

    Google Scholar 

  46. G. G. LUGERO, V. DE FALCO CAPARBO, M. L. GUZZO, B. KÖNIG and V. JORGETTI, Implant Dent. 9 (2000) 303

    Article  CAS  Google Scholar 

  47. M. YAMAZAKI, T. SHIROTA, Y. TOKUGAWA, M. MOTOHASHI, K. OHNO, K. MICHI and A. YAMAGUCHI, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 87 (1999) 411

    Article  CAS  Google Scholar 

  48. A. DEBENEDITTIS, M. MATTIOLI-BELMONTE, A. KRAJEWSKI, M. FINI, A. RAVAGLIOLI, R. GIARDINO and G. BIAGINI, Int. J. Artif. Organs 22 (1999) 516

    CAS  Google Scholar 

  49. H. MORI, M. MANABE, Y. KURACHI and M. NAGUMO, J. Oral Maxillofac. Surg. 55 (1997) 351

    Article  CAS  Google Scholar 

  50. M. FINI, I. NICOL, N. ALDINI, M. G. GANDOLFI, M. MATTIOLI BELMONTE, G. GIAVARESI, C. ZUCCHINI, A. DEBENEDITTIS, S. AMATI, A. RAVAGLIOLI, A. KRAYEWSKI, M. ROCCA, G. A. GUZZARDELLA, G. BIAGINI and R. GIARDINO, Int. J. Artif. Organs. 20 (1997) 291

    CAS  Google Scholar 

  51. K. HAYASHI, K. UENOYAMA, T. MASHIMA and Y. SUGIOKA, Biomaterials 15 (1994) 11

    Article  CAS  Google Scholar 

  52. J. PAN, T. SHIROTA, K. OHNO and K. MICHI, J. Oral Maxillofac. Surg. 58 (2000) 877

    Article  CAS  Google Scholar 

  53. J. W. WANG, W. LI, S. W. XU, D. S. YANG, Y. WANG, M. LIN and G. F. ZHAO, Clin. J. Traumatol. 8 (2005) 111

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, Institute of Biology, State University of Campinas, P.O. Box 6109, Campinas, SP, 13084-971, Brazil

    Marcelo Rodrigues Cunha, Arnaldo Rodrigues Santos Jr. & Selma C. Genari

  2. Institute of Biological Sciences, University of Pinhal, Espirito Santo do Pinhal, SP, Brazil

    Marcelo Rodrigues Cunha, Arnaldo Rodrigues Santos Jr. & Selma C. Genari

  3. Department of Applied Biology, State University of São Paulo, Jaboticabal, SP, Brazil

    Arnaldo Rodrigues Santos Jr.

  4. Department of Chemistry and Molecular Physics, Institute of Chemistry, University of São Paulo, Sao Carlos, SP, Brazil

    Gilberto Goissis

Authors
  1. Marcelo Rodrigues Cunha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arnaldo Rodrigues Santos Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gilberto Goissis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Selma C. Genari
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcelo Rodrigues Cunha.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cunha, M.R., Santos, A.R., Goissis, G. et al. Implants of polyanionic collagen matrix in bone defects of ovariectomized rats. J Mater Sci: Mater Med 19, 1341–1348 (2008). https://doi.org/10.1007/s10856-006-0105-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-0105-y

Keywords

Search

Navigation