Abstract
Hydroxyapatite (HA) spherules and autologous bone (AB) with a central vascular pedicle were housed inside an HA-chamber to form the skeletal segment of specific shape. Experimental chambers were then inserted in a pocket between medial thigh muscles in 13 New Zealand male rabbits for 3 months. Three graft group were scheduled: (A) HA and AB without vascular pedicle, (B) HA with vascular pedicle, (C) HA and AB with vascular pedicle. At term, histology showed tissue and cellular degeneration in group A chambers. Due to spherules coalescence, fibrous tissue is formed in group B chambers. Group C chambers contained living osteocytes in the implanted bone, several newly formed vessels in soft tissue, bone and partial hydroxyapatite erosions. New bone was formed in apposition to both autologous bone and hydroxyapatite. Our study suggests that this experimental model could be used to grow adequately sized vascularized skeletal segments.
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Acknowledgements
The authors wish to thank Mr. Vincenzo Molino for his remarkable technical assistance in animal care and treatment, and the “Centro interdipartimentale Grandi Strumenti” (CIGS) of the University of Modena e Reggio Emilia for SEM availability and assistance. The Research University Fund (FAR) of the Department of Anatomy and Histology, University of Modena and Reggio Emilia supported this investigation.
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Tarallo, L., Zaffe, D., Adani, R. et al. Extracorporeal hydroxyapatite-chamber for bone and biomaterial studies. J Mater Sci: Mater Med 19, 159–166 (2008). https://doi.org/10.1007/s10856-006-0102-1
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DOI: https://doi.org/10.1007/s10856-006-0102-1