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In vitro biodegradation and biocompatibility of gelatin/montmorillonite-chitosan intercalated nanocomposite

Journal of Materials Science: Materials in Medicine volume 18, pages 951–957 (2007)Cite this article

Abstract

The intercalated nanocomposite of gelatin/montmorillonite-chitosan (Gel/MMT-CS) was prepared via the solution intercalation process. In vitro degradation tests showed that the nanocomposite had a lower degradation rate than Gel-CS composite. And the introduced intercalation structure endowed Gel/MMT-CS nanocomposite with a controllable degradation rate when changing the MMT content. Cells attachment, spread and proliferation on the Gel/MMT-CS membranes were investigated by scanning electron microscopy (SEM) and mitochondrial activity assay. The results provided evidences of good adhesion, proliferation and morphology of rat stromal stem cells on Gel/MMT-CS membranes compared to the tissue culture plates (TCPs), making the Gel/MMT-CS nanocomposite a promising candidate towards tissue engineering.

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References

  1. D. F. WILLIAMS, 1999, The Williams dictionary of biomaterials, (Liverpool, UK: University Press) p. 40

  2. L. J. CHEN and M. WANG, Biomaterials 23 (2002) 2631

    Article  CAS  Google Scholar 

  3. J. CRESPO, M. A. SATORRE, J. A. QUINTANA and F. ANIA, J. Mater. Sci. 30 (1995) 6145

    Article  CAS  Google Scholar 

  4. R. A. A. MUZZARELLI, Carbohydrate Polym. 20 (1993) 7

    Article  CAS  Google Scholar 

  5. Y. L. CUI, A. D. QI and W. G. LIU, Biomaterials 24 (2003) 3859

    Article  CAS  Google Scholar 

  6. S. HSU, S. W. WHU and C. L. TSAI, J. Polym. Res. 11 (2004) 141

    Article  CAS  Google Scholar 

  7. A. BORZACCHIELLO, L. AMBROSIO, P. A. NETTI and J. SAN ROMAN, J. Mater. Sci. 12 (2001) 861

    Article  CAS  Google Scholar 

  8. H. TAKEUCHI, T. YASUJI H., YAMAMOTO and Y. KAWASHIMA, Pharm. Res. 17 (2000) 94

    Article  CAS  Google Scholar 

  9. K. LI, Y. WANG, Z. C. MIAO and M. F. FENG, Biotechnol. Lett. 26 (2004) 879

    Article  CAS  Google Scholar 

  10. K. D. YAO, H. L. TU, F. CHENG and J. LIU, Angew. Makromol. Chem. 63 (1997) 245

    Google Scholar 

  11. E. PIANIGIANI, A. ANDREASSI and P. TADDEUCCI, Biomaterials 20 (1999) 1689

    Article  CAS  Google Scholar 

  12. W. W. LU, F. ZHAO, K. M. C. CHEUNG and K. D. YAO, J. Mater. Sci. Mater. Med. 14 (2003) 1039

    Article  CAS  Google Scholar 

  13. N. SHANMUGASUNDARAM, P. PAVICHANDRAN, P. NEELAKANTA REDDY and P. RAO, Biomaterials 22 (2001) 1943

    Article  CAS  Google Scholar 

  14. A. USUKI, Y. KOJIMA, M. KAWASUMI and O. KAMIGAITO, J. Mater. Res. 8 (1993) 1179

    CAS  Google Scholar 

  15. M. KAWASUMI, N. HASEGAWA and A. OKADA, Macromolecules 30 (1997) 6333

    Article  CAS  Google Scholar 

  16. Y. KOJIMA, A. USUKI, M. KAWASUMI, A. OKADA, Y. FUKUSHIMA and T. KURAUCHI, J. Mater. Res. 8 (1993) 1185

    Google Scholar 

  17. K. YANO, A. USUKI and A. OKADA, J. Polym. Sci. [A1]. 35 (1997) 2289

    CAS  Google Scholar 

  18. F. H. LIN, Y. H. LEE and C. H. JIAN, Biomaterials 23 (2002) 1981

    Article  CAS  Google Scholar 

  19. J. H. LEE, T. G. PARK and Y. K. LEE, Biomaterials 24 (2003) 2773

    Article  CAS  Google Scholar 

  20. J. P. ZHENG, L. F. Xi, H. L. ZHANG and K. D. YAO, J. Mater. Sci. Lett. 22 (2003) 1179

    Article  CAS  Google Scholar 

  21. J. E. BABENSEE, J. M. ANDERSON, L. V. MELNTIRE and A. G. MIKOS, Adv. Drug. Deliv. Rev. 33 (1998) 111

    Article  Google Scholar 

  22. A. DOMARD and M. RINAUDO, Int. J. Biol. Macromol. 5 (1983) 49

    Article  CAS  Google Scholar 

  23. C. S. CHEN, M. MRKSICH and D. E. INGBER, Biotechnol. Prog. 14 (1998) 356

    Article  CAS  Google Scholar 

  24. S. VERRIER, J. J. BLAKER, V. MAQUET and A. R. BOCCACCINI, Biomaterials 25 (2004) 3013

    Article  Google Scholar 

  25. K. ANSELME, Biomaterials 21 (2000) 667

    Article  CAS  Google Scholar 

  26. M. HOTT, B. NOEL, D. BERNACHE-ASSOLANT and P. J. MARIE, J. Biomed. Mater. Res. 37 (1997) 508

    Article  Google Scholar 

  27. C. CHATELET, O. DAMOUR and A. DOMARD, Biomaterials 22 (2001) 261

    Article  CAS  Google Scholar 

  28. T. MOSMANN, J. Immunol. Methods 65 (1983) 55

    Article  CAS  Google Scholar 

  29. J. S. MAO, X. H. WANG and K. D. YAO, J. Mater. Sci. 38 (2003) 2283

    Article  CAS  Google Scholar 

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Acknowledgment

The authors wish to thank the National Natural Science Foundation of China, through Grant No. 20204008, for supporting this research.

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Affiliations

  1. College of Materials Science and Engineering, Tianjin University, Tianjin, 300072, P.R. China

    Hong Zhuang, Jun Ping Zheng, Hong Gao & Kang De Yao

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  1. Hong Zhuang
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  2. Jun Ping Zheng
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  3. Hong Gao
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  4. Kang De Yao
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Correspondence to Jun Ping Zheng.

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Zhuang, H., Zheng, J.P., Gao, H. et al. In vitro biodegradation and biocompatibility of gelatin/montmorillonite-chitosan intercalated nanocomposite. J Mater Sci: Mater Med 18, 951–957 (2007). https://doi.org/10.1007/s10856-006-0093-y

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  • DOI: https://doi.org/10.1007/s10856-006-0093-y

Keywords

  • Chitosan
  • Gelatin
  • Lysozyme
  • Intercalate Nanocomposite
  • High Gelatin