Skip to main content
SpringerLink
Log in

Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

In the field of bone tissue engineering there is a high demand on bone graft materials which promote bone formation. By combination of collagen type I with nanocrystalline hydroxyapatite (HA) we generated a resorbable material which structure and composition is close to those of the extracellular bone matrix. This nanocomposit material was produced in a biomimetic process in which collagen fibril assembly and mineralisation with hydroxyapatite occur simultaneously. In this study the proliferation and osteogenic differentiation of human bone marrow-derived stromal cells (hBMSC) on membranes of biomimetically mineralised collagen type I was investigated. To this end, we optimised biochemical assays for determination of cell number and alkaline phosphatase activity corresponding to the special properties of this biomaterial. For cell experiments hBMSC were seeded on the mineralised collagen membranes and cultivated over 35 days, both in static and perfusion culture, in the presence and absence of dexamethasone, β-glycerophosphate and ascorbate. Compared to cells grown on tissue culture polystyrene we found attenuated proliferation rates, but markedly increased activity of alkaline phosphatase on the mineralised collagen indicating its promoting effect on the osteogenic differentiation of hBMSC. Therefore this bone-like material may act as a suitable artificial extracellular matrix for bone tissue engineering. Perfusion of the 2D cell matrix constructs with cell culture medium did not improve proliferation and osteogenic differentiation of the hBMSC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. A. EL-GHANNAM, Expert. Rev. Med. Devices. 2 (2005) 87

    Article  Google Scholar 

  2. J. BRADT, M. MERTIG, A. TERESIAK and W. POMPE, Chem. Mater. 11 (1999) 2694

    Article  CAS  Google Scholar 

  3. R. BURTH, M. GELINSKY and W. POMPE, Tech. Textile. 8 (1999) 20

    Google Scholar 

  4. M. GELINSKY, U. KÖNIG, A. SEWING and W. POMPE, Matwiss. Werkstofftech. 35 (2004) 229

    Article  CAS  Google Scholar 

  5. H. SCHLIEPHAKE, F. TAVASSOL, M. GELINSKY, M. DARD, A. SEWING, and W. POMPE, Clin. Oral Implants Res. 15 (2004) 112

    Article  Google Scholar 

  6. R. MAI, M. G. HAGEDORN, M. GELINSKY, C. WERNER, D. TURHANI, H. SPÄTH, Th. GEDRANGE, and G. LAUER, J. Cranio-Maxillofac. Surg. 34 (2006) 101

    Article  Google Scholar 

  7. H. DOMASCHKE, M. GELINSKY, B. BURMEISTER, R. FLEIG, T. HANKE, A. REINSTORF, W. POMPE and A. RÖSEN-WOLFF, Tissue Eng. 12 (2006) 949

    Article  CAS  Google Scholar 

  8. Y. JIANG, B. N. JAHAGIRDAR, R. L. REINHARDT, R. E. SCHWARTZ, C. D. KEENE, X. R. ORTIZ-GONZALEZ, M. REYES, T. LENVIK, T. LUND, M. BLACKSTAD, J. DU, S. ALDRICH, A. LISBERG, W. C. LOW, D. A. LARGAESPADA and C. M. VERFAILLIE, Nature 418 (2002) 41

    Article  CAS  Google Scholar 

  9. M. F. PITTENGER, A. M. MACKAY, S. C. BECK, R. K. JAISWAL, R. DOUGLAS, J. D. MOSCA, M. A. MOORMAN, D. W. SIMONETTI, S. CRAIG and D. R. MARSHAK, Science 284 (1999) 143

    Article  CAS  Google Scholar 

  10. S.P. BRUDER, N. JAISWAL and S. E. HAYNESWORTH, J. Cell. Biochem. 64 (1997) 278

    Article  CAS  Google Scholar 

  11. N. JAISWAL, S. E. HAYNESWORTH, A. I. CAPLAN and S. P. BRUDER, J. Cell. Biochem. 64 (1997) 295

    Article  CAS  Google Scholar 

  12. S. C. MENDES, J. M. TIBBE, M. VEENHOF, S. BOTH, F. C. ONER, C. A. van BLITTERSWIJK and J. D. de BRUIJN, J. Mater. Sci. Mater. Med. 15 (2004) 1123

    Article  CAS  Google Scholar 

  13. J. OSWALD, S. BOXBERGER, B. JORGENSEN, S. FELDMANN, G. EHNINGER, M. BORNHAUSER and C. WERNER, Stem Cells 22 (2004) 377

    Article  Google Scholar 

  14. A. SABOKBAR, P. J. MILLETT, B. MYER and N. RUSHTON, Bone Miner. 27 (1994) 57

    Article  CAS  Google Scholar 

  15. H. HATTORI, M. SATO, K. MASUOKA, M. ISHIHARA, T. KIKUCHI, T. MATSUI, B. TAKASE, T. ISHIZUKA, M. KIKUCHI, K. FUJIKAWA and M. ISHIHARA, Cells Tissues Organs. 178 (2004) 2

    Article  Google Scholar 

  16. P. NIEMEYER, U. KRAUSE, J. FELLENBERG, P. KASTEN, A. SECKINGER, A. D. HO and H. G. SIMANK, Cells Tissues Organs. 177 (2004) 68

    Article  CAS  Google Scholar 

  17. S. P. BRUDER, D. J. FINK and A. I. CAPLAN, J. Cell. Biochem. 56 (1994) 283

    Article  CAS  Google Scholar 

  18. M. JAGER, T. FESER, H. DENCK and R. KRAUSPE, Ann. Biomed. Eng. 33 (2005) 1319

    Article  CAS  Google Scholar 

  19. C. A. MCCULLOCH and H. C. TENENBAUM, Anat. Rec. 215 (1986) 397

    Article  CAS  Google Scholar 

  20. G. J. ATKINS, P. KOSTAKIS, B. PAN, A. FARRUGIA, S. GRONTHOS, A. EVDOKIOU, K. HARRISON, D. M. FINDLAY and A. C. ZANNETTINO, J. Bone Miner. Res. 18 (2003) 1088

    Article  CAS  Google Scholar 

  21. S. WALSH, C. JEFFERISS, K. STEWART, G. R. JORDAN, J. SCREEN and J. N. BERESFORD, Bone 27 (2000) 185

    Article  CAS  Google Scholar 

  22. S. H. CARTMELL, B. D. PORTER, A. J. GARCIA and R. E. GULDBERG, Tissue Eng. 9 (2003) 1197

    Article  CAS  Google Scholar 

  23. S. M. MUELLER, S. MIZUNO, L. C. GERSTENFELD and J. GLOWACKI, J. Bone Miner. Res. 14 (1999) 2118

    Article  CAS  Google Scholar 

  24. M. WIEDMANN-AL-AHMAD, R. GUTWALD, N. C. GELLRICH, U. HUBNER and R. SCHMELZEISEN, J. Mater. Sci. Mater. Med. 16 (2005) 57

    Article  CAS  Google Scholar 

  25. G. N. BANCROFT, V. I. SIKAVITSAS, J. van den DOLDER, T. L. SHEFFIELD, C. G. AMBROSE, J. A. JANSEN and A. G. MIKOS, Proc. Natl. Acad. Sci. U S A. 99 (2002) 12600

    Article  CAS  Google Scholar 

  26. H. L. HOLTORF, J. A. JANSEN and A. G. MIKOS, J. Biomed. Mater. Res. A. 72 (2005) 326

    Google Scholar 

  27. H. HOSSEINKHANI, Y. INATSUGU, Y. HIRAOKA, S. INOUE and Y. TABATA, Tissue Eng. 11 (2005) 1476

    Article  CAS  Google Scholar 

  28. M. FUSS, E. M. EHLERS, M. RUSSLIES, J. ROHWEDEL and P. BEHRENS, Ann. Anat. 182 (2000) 303

    Article  CAS  Google Scholar 

  29. K. NISHIO, M. NEO, H. AKIYAMA, S. NISHIGUCHI, H. M. KIM, T. KOKUBO and T. NAKAMURA, J. Biomed. Mater. Res. 52 (2000) 652

    Article  CAS  Google Scholar 

  30. R. SHU, R. MCMULLEN, M. J. BAUMANN and L. R. MCCABE, J. Biomed. Mater. Res. A. 67 (2003) 1196

    Article  CAS  Google Scholar 

  31. E. NORDSTROM, H. OHGUSHI, T. YOSHIKAWA, A. T. YOKOBORI and T. YOKOBORI, Biomed. Mater. Eng. 9 (1999) 21

    CAS  Google Scholar 

  32. R. O. OREFFO, F. C. DRIESSENS, J. A. PLANELL and J. T. TRIFFITT, Biomaterials 19 (1998) 1845

    Article  CAS  Google Scholar 

  33. D. D. DELIGIANNI, N. D. KATSALA, P. G. KOUTSOUKOS and Y. F. MISSIRLIS, Biomaterials 22 (2001) 87

    Article  CAS  Google Scholar 

  34. Y. F. CHOU, J. C. DUNN and B. M. WU, J. Biomed. Mater. Res. B. 75 (2005) 81

    Google Scholar 

  35. P. BERUBE, Y. YANG, D. L. CARNES, R. E. STOVER, E. J. BOLAND and J. L. ONG, J. Periodontol. 76 (2005) 1697

    Article  CAS  Google Scholar 

  36. K. L. KILPADI, P. L. CHANG and S. L. BELLIS, J. Biomed. Mater. Res. 57 (2001) 258

    Article  CAS  Google Scholar 

  37. R. M. SALASZNYK, W. A. WILLIAMS, A. BOSKEY, A. BATORSKY and G. E. PLOPPER, J. Biomed. Biotechnol. 1 (2004) 24

    Article  Google Scholar 

  38. M. MIZUNO, R. FUJISAWA and Y. KUBOKI, J. Cell. Physiol. 184 (2000) 207

    Article  CAS  Google Scholar 

  39. X. B. YANG, R. S. BHATNAGAR, S. LI and R. O. OREFFO, Tissue Eng. 10 (2004) 1148

    CAS  Google Scholar 

  40. J. GEORGE, Y. KUBOKI and T. MIYATA, Biotechnol. Bioeng. 95 (2006) 404

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful to Ortrud Zieschang for preparation of the tapes. We thank Antje Vogel for the SEM investigation. This work was supported by the “Sächsisches Ministerium für Wissenschaft und Kunst” (Saxonian Ministry for Science and Arts, SMWK).

Author information

Authors and Affiliations

  1. Max Bergmann Center of Biomaterials, Institute of Materials Science, Technische Universität Dresden, Budapester-Str. 27, 01069, Dresden, Germany

    Anne Bernhardt, Anja Lode, Wolfgang Pompe & Michael Gelinsky

  2. Medizinische Klinik und Poliklinik I, University Hospital Carl Gustav Carus, Fetscherstr. 74, 01307, Dresden, Germany

    Sabine Boxberger

Authors
  1. Anne Bernhardt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anja Lode
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sabine Boxberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wolfgang Pompe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Michael Gelinsky
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anne Bernhardt.

Additional information

Anne Bernhardt and Anja Lode contributed equally to this paper

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bernhardt, A., Lode, A., Boxberger, S. et al. Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells. J Mater Sci: Mater Med 19, 269–275 (2008). https://doi.org/10.1007/s10856-006-0059-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-0059-0

Keywords

Search

Navigation