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Competitive adsorption of bovine serum albumin and lysozyme on characterized calcium phosphates by polyacrylamide gel electrophoresis method

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Abstract

Characterizations of hydroxyapatite (HA), biphasic calcium phosphate (BCP) and beta tricalcium phosphate (β-TCP) ceramic particles were carried out using X-ray diffusion (XRD), Scanning electron micrograph (SEM), Particle Sizer and Zeta potential analyzer. Competitive adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the three calcium phosphates were investigated by polyacrylamide gel electrophoresis (PAGE) method. The results showed that HA, BCP and β-TCP ceramic particles with irregular shapes and similar size distributions all had negative surface net charges in pH7.4 phosphate buffered saline (PBS) solution and exhibited alike behaviors of BSA and LSZ adsorption. LSZ had higher affinity for calcium phosphate ceramics than BSA and its adsorption on them didn’t be almost influenced by the increasing of BSA concentration in the solution. Electrostatic interaction played an important role on the competitive adsorption of BSA and LSZ on the surface of calcium phosphate ceramic particles.

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Acknowledgements

This work has been financially supported by National Basic Research Program of China (Contract No: G2005cb623901) and National Natural Science Foundation of China (Contract No: 50473051).

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Authors and Affiliations

  1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China

    X. D. Zhu, H. S. Fan, C. Y. Zhao, J. Lu & X. D. Zhang

  2. Biomaterials Research Center, National Institute for Materials Science, Tsukuba, Ibaraki, Japan

    T. Ikoma & J. Tanaka

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  1. X. D. Zhu
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  2. H. S. Fan
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  3. C. Y. Zhao
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Correspondence to H. S. Fan.

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Zhu, X.D., Fan, H.S., Zhao, C.Y. et al. Competitive adsorption of bovine serum albumin and lysozyme on characterized calcium phosphates by polyacrylamide gel electrophoresis method. J Mater Sci: Mater Med 18, 2243–2249 (2007). https://doi.org/10.1007/s10856-006-0057-2

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  • DOI: https://doi.org/10.1007/s10856-006-0057-2

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