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Simultaneous electrochemical detection of dopamine and hydrogen peroxide based on bifunctional microelectrode array

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Abstract

Reactive oxygen species (ROS) and dopamine (DA) can serve as two important biomarkers in the diagnosis of Parkinson’s disease (PD). Due to the close relationship between DA and hydrogen peroxide (H2O2) in the process of PD occurrence and development, the simultaneous detection of DA and H2O2 is of great significance for the diagnosis and treatment of PD. In this paper, we report a high integrated bifunctional microelectrode array (MEA) for the simultaneous detection of DA and H2O2. The sensing electrodes were selectively modified with PEDOT film and Pt-black/PEDOT by electrochemical deposition for detecting DA (with a sensitivity of 38.1 nA/μM/mm2) and H2O2 (with a sensitivity of 45.2 nA/μM/mm2), respectively. The MEA exhibited good sensitivity, selectivity and was capable of sensing both DA and H2O2 simultaneously in serum without any crosstalk. This work proved the feasible of utilizing MEA for multicomponent analysis and showed great potential of MEA for in vitro analysis as well as early diagnosis of PD.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (No. DUT22YG233); National Natural Science Foundation of China (No. 22274015); 2022 Provincial Natural Science Foundation Plan (No. 2022-MS-151).

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Authors and Affiliations

  1. School of Chemical Engineering, Dalian University of Technology, Dalian, 116023, People’s Republic of China

    Hongyu Pang, Bing Yin, Xiaobo Liu, Tingting Jiang & Shuo Wu

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  1. Hongyu Pang
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  2. Bing Yin
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  3. Xiaobo Liu
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  4. Tingting Jiang
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  5. Shuo Wu
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Contributions

HP: validation, formal analysis, investigation, methodology, data curation, writing–original draft, and visualization. BY: methodology, writing–review & editing, visualization, and funding acquisition. XL: methodology, investigation, and project administration. TJ: conceptualization, resources, and project administration. SW: resources, writing–review & editing, supervision, project administration, and funding acquisition.

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Correspondence to Bing Yin or Shuo Wu.

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Pang, H., Yin, B., Liu, X. et al. Simultaneous electrochemical detection of dopamine and hydrogen peroxide based on bifunctional microelectrode array. J Mater Sci: Mater Electron 34, 883 (2023). https://doi.org/10.1007/s10854-023-10282-4

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