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Glucose-responsive poly(vinyl alcohol)/β-cyclodextrin hydrogel with glucose oxidase immobilization

  • Materials for life sciences
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Abstract

Glucose-responsive poly(vinyl alcohol)/β-cyclodextrin (PVA/β-CD) hydrogels cross-linked by citric acid were prepared through an environment-friendly synthesis procedure. The glucose oxidase (GOx) was physically immobilized within the β-CD cavity for accurate detection of interstitial fluid glucose levels reaching approximately 1 mM. We evaluated the viscoelastic behavior of the PVA/β-CD solutions through dynamic oscillatory shear testing, and the PVA/β-CD/GOx hydrogel 10 shows the optimal properties with excellent water absorption (312 ± 17%), low solubility, and long moisture retention time (approximately 4.5 h for complete drying at 37 °C). The PVA/β-CD/GOx hydrogel showed the high flexural and tensile strengths of ≥ 400 and ≥ 5 MPa, respectively. Furthermore, the fabricated PVA/β-CD/GOx hydrogel 10 displayed a linear amperometric response (R2 is 0.984) in the glucose concentration range from 1.0 to 5.0 mM with a relatively high sensitivity of 7.58 µA mM−1 and a low detection limit of 5.141 × 10−4 M at low applied potential (0.27 V vs. Ag/AgCl coated with Prussian blue), indicating its great potential as a patch sensor for noninvasive glucose monitoring.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIT) (No. R-2018-00235).

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Authors and Affiliations

  1. Department of Fiber-system Engineering, Dankook University, 152, Jookjeon-ro, Suji-gu, Yongin-si, Gyeonggi-do, 448-701, Republic of Korea

    Geon Jin Kim, Kee Jong Yoon & Kyu Oh Kim

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  1. Geon Jin Kim
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  3. Kyu Oh Kim
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Correspondence to Kyu Oh Kim.

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Kim, G.J., Yoon, K.J. & Kim, K.O. Glucose-responsive poly(vinyl alcohol)/β-cyclodextrin hydrogel with glucose oxidase immobilization. J Mater Sci 54, 12806–12817 (2019). https://doi.org/10.1007/s10853-019-03805-0

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