Cell attachment/detachment behavior on poly(N-isopropylacrylamide)-based microgel films: the effect of microgel structure and swelling ratio
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Microgels are cross-linked soft particles with a three-dimensional network structure that are swollen in a good solvent. Poly(N-isopropylacrylamide) (pNIPAAm)-based microgels have attracted great attentions for their temperature responsive property, particularly in recent years, pNIPAAm-based microgel films were utilized as a new kind of thermoresponsive surface to tune cell attachment/detachment behavior via temperature stimuli. However, some results are not consistent, for example, different polymerization conditions may bring out different results even for pure pNIPAAm microgel. This work aims to find out which factor plays the critical role for successful cell detachment on the pNIPAAm-based microgel films. The results unraveled that the structure and swelling ratio of the microgel rather than the film thickness plays a key role on the successful cells detachment, unlike linear pNIPAAm films in which the cells’ attach/detach property is only determined by the film thickness. For poly(N-isopropylacrylamide–styrene) microgel film, NIH3T3 cells could only detach when the microgel has a uniform structure and the volume dilatation of the microgel (20/38 °C) is larger than 4.
The authors gratefully acknowledge the financial support from the Natural Science Fund of Shandong Province (ZR2015BM013) and the National Natural Science Foundation of China (21773310).
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Conflict of interest
The authors declare that they have no conflict of interest.
- 3.Ohtsuki K, Miyai S, Yamaguchi A, Morikawa K, Okano T (2012) Biochemical characterization of novel lignans isolated from the wood of Taxus yunnanensis as effective stimulators for glycogen synthase kinase-3beta and the phosphorylation of basic brain proteins by the kinase in vitro. Biol Pharm Bull 35:385–393CrossRefGoogle Scholar
- 9.Nandkumar MA, Yamato M, Kushida A, Konno C, Hirose M, Kikuchi A, Okano T (2002) Two-dimensional cell sheet manipulation of heterotypically co-cultured lung cells utilizing temperature-responsive culture dishes results in long-term maintenance of differentiated epithelial cell functions. Biomaterials 23:1121–1130CrossRefGoogle Scholar
- 28.Nash ME, Carroll WM, Nikoloskya N, Yang R, O’Connell C, Gorelov AV, Dockery P, Liptrot C, Lyng FM, Garcia A, Rochev YA (2011) Straightforward, one-step fabrication of ultrathin thermoresponsive films from commercially available pNIPAm for cell culture and recovery. ACS Appl Mater Interfaces 3:1980–1990CrossRefGoogle Scholar