Journal of Materials Science

, Volume 53, Issue 9, pp 6302–6312 | Cite as

A high strength semi-degradable polysaccharide-based hybrid hydrogel for promoting cell adhesion and proliferation

  • Hongbo Wang
  • Ziyang Xu
  • Yuanhao Wu
  • Haofei Li
  • Wenguang Liu
Biomaterials
  • 45 Downloads

Abstract

Traditionally, practical applications of polysaccharide hydrogels have been limited for their weak mechanical properties under physiological conditions. In this study, we constructed a novel polysaccharide-based semi-degradable hydrogel whose network was constructed by chemical cross-linking of glycidyl methacrylate-modified laminarin and the hydrogen bonded physical cross-linking of poly(N-acryloyl glycinamide). In addition, the introduction of 1-vinyl-1,2,4-triazole content could increase the equilibrium water content of hydrogels and endow hydrogels with anti-bacterial and anti-inflammatory abilities. The prepared hydrogels exhibited comprehensive high mechanical properties up to 0.63 MPa tensile strength, 650% stretchability, and maximum 3.2 MPa compressive strength at swelling equilibrium state. The hydrogen bond interactions could well support the three-dimensional network of hydrogel after the degradation of modified laminarin. Meanwhile, the content of laminarin could facilitate cell adhesion and proliferation on the surface of hydrogel. It is anticipated that this high strength semi-degraded hydrogel may find a promising application as articular cartilage replacement.

Notes

Acknowledgements

The authors gratefully acknowledge the support for this work from National Natural Science Foundation (Grant Nos. 51325305, 51733006, 51303132), National Key Research and Development Program (Grant No. 2016YFC1101301).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10853_2018_2019_MOESM1_ESM.doc (3.8 mb)
Supplementary material 1 (DOC 3931 kb)

References

  1. 1.
    Gong JP, Katsuyama Y, Kurokawa T, Osada Y (2003) Double-network hydrogels with extremely high mechanical strength. Adv Mater 15:1155–1158CrossRefGoogle Scholar
  2. 2.
    Ito K (2007) Novel cross-linking concept of polymer network: synthesis, structure, and properties of slide-ring gels with freely movable junctions. Polym J 39:489–499CrossRefGoogle Scholar
  3. 3.
    Haraguchi K (2007) Nanocomposite hydrogels. Curr Opin Solid State Mater Sci 11:47–54CrossRefGoogle Scholar
  4. 4.
    Henderson KJ, Zhou TC, Otim KJ, Shull KR (2010) Ionically cross-linked triblock copolymer hydrogels with high strength. Macromolecules 43:6193–6201CrossRefGoogle Scholar
  5. 5.
    Li W, An H, Tan Y et al (2012) Hydrophobically associated hydrogels based on acrylamide and anionic surface active monomer with high mechanical strength. Soft Matter 8:5078–5086CrossRefGoogle Scholar
  6. 6.
    Sakai T, Matsunaga T, Yamamoto Y et al (2008) Design and fabrication of a high-strength hydrogel with ideally homogeneous network structure from tetrahedron-like macromonomers. Macromolecules 41:5379–5384CrossRefGoogle Scholar
  7. 7.
    Huang T, Xu H, Jiao K et al (2007) A novel hydrogel with high mechanical strength: a macromolecular microshpere composite hydrogel. Adv Mater 19:1622–1626CrossRefGoogle Scholar
  8. 8.
    Wang Q, Zhang YY, Dai XY, Shi XH, Liu WG (2017) A high strength pH responsive supramolecular copolymer hydrogel. Sci China Tech Sci 60:78–83CrossRefGoogle Scholar
  9. 9.
    Gasperini L, Mano JF, Reis RL (2014) Natural polymers for the microencapsulation of cells. J R Soc Interface 11:20140817CrossRefGoogle Scholar
  10. 10.
    Mano JF, Silva GA, Azevedo HS et al (2007) Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J R Soc Interface 4:999–1030CrossRefGoogle Scholar
  11. 11.
    Senni K, Pereira J, Gueniche F et al (2011) Marine polysaccharides: a source of bioactive molecules for cell therapy and tissue engineering. Mar Drugs 9:1664–1681CrossRefGoogle Scholar
  12. 12.
    Silva TH, Ferreira BM, Oliveira JM et al (2012) Materials of marine origin a review on polymers and ceramics of biomedical interest. Int Mater Rev 57:276–307CrossRefGoogle Scholar
  13. 13.
    Alderkamp AC, Rijssel M, Bolhuis H (2007) Characterization of marine bacteria and the activity of their enzyme systems involved in degradation of the algal storage glucan laminarin. FEMS Microbiol Ecol 59:108–117CrossRefGoogle Scholar
  14. 14.
    Shin HJ, Oh JS, Kim SI, Kim HW, Son JH (2009) Conformational characteristics of beta-glucan in laminarin probed by terahertz spectroscopy. Appl Phys Lett 94:111911–111913CrossRefGoogle Scholar
  15. 15.
    Kim KH, Kim YW, Kim HB, Lee BJ, Lee DS (2006) Anti-apoptotic activity of laminarin polysaccharides and their enzymatically hydrolyzed oligosaccharides from Laminaria japonica. Biotechnol Lett 28:439–446CrossRefGoogle Scholar
  16. 16.
    Menshova RV, Ermakova SP, Anastyuk SD et al (2014) Structure, enzymatic transformation and anticancer activity of branched high molecular weight laminaran from brown alga Eisenia bicyclis. Carbohydr Polym 99:101–109CrossRefGoogle Scholar
  17. 17.
    Ermakova S, Men’shova R, Vishchuk O et al (2013) Water-soluble polysaccharides from the brown alga Eisenia bicyclis Structural characteristics and antitumor activity. Algal Res 2:51–58CrossRefGoogle Scholar
  18. 18.
    Custódio CA, Reis RL, Mano JF (2016) Photo-cross-linked laminarin-based hydrogels for biomedical applications. Biomacromolecules 17:1602–1609CrossRefGoogle Scholar
  19. 19.
    Fan M, Ma Y, Tan H et al (2017) Covalent and injectable chitosan-chondroitin sulfate hydrogels embedded with chitosan microspheres for drug delivery and tissue engineering. Mater Sci Eng C Mater Biol Appl 71:67–74CrossRefGoogle Scholar
  20. 20.
    Hu J, Quan Y, Lai Y et al (2017) A smart aminoglycoside hydrogel with tunable gel degradation, on-demand drug release, and high antibacterial activity. J Control Release 247:145–152CrossRefGoogle Scholar
  21. 21.
    Wei Z, Zhao J, Chen YM, Zhang P, Zhang Q (2016) Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells. Sci Rep 6:37841–37852CrossRefGoogle Scholar
  22. 22.
    Travan A, Scognamiglio F, Borgogna M et al (2016) Hyaluronan delivery by polymer demixing in polysaccharide-based hydrogels and membranes for biomedical applications. Carbohydr Polym 150:408–418CrossRefGoogle Scholar
  23. 23.
    Liu J, Qi C, Tao K et al (2016) Sericin/dextran injectable hydrogel as an optically trackable drug delivery system for malignant melanoma treatment. ACS Appl Mater Interfaces 8:6411–6422CrossRefGoogle Scholar
  24. 24.
    Cho IS, Cho MO, Li Z et al (2016) Synthesis and characterization of a new photo-crosslinkable glycol chitosan thermogel for biomedical applications. Carbohydr Polym 144:59–67CrossRefGoogle Scholar
  25. 25.
    Xu J, Tam M, Samaei S et al (2017) Mucoadhesive chitosan hydrogels as rectal drug delivery vessels to treat ulcerative colitis. Acta Biomater 48:247–257CrossRefGoogle Scholar
  26. 26.
    Dai X, Zhang Y, Gao L et al (2015) A mechanically strong, highly stable, thermoplastic, and self-healable supramolecular polymer hydrogel. Adv Mater 27:3566–3571CrossRefGoogle Scholar
  27. 27.
    Colanceska-Ragenovic K, Dimova V, Kakurinov V, Molnar DG, Buzarovsk A (2001) Synthesis, antibacterial and antifungal activity of 4-substituted-5-aryl-1,2,4-triazoles. Molecules 6:815–824CrossRefGoogle Scholar
  28. 28.
    Sztanke K, Tuzimski T, Rzymowska J, Pasternak K, Kandefer-Szerszeń M (2008) Synthesis, determination of the lipophilicity, anticancer and antimicrobial properties of some fused 1,2,4-triazole derivatives. Eur J Med Chem 43:404–419CrossRefGoogle Scholar
  29. 29.
    Vijesh AM, Isloor AM, Shetty P, Sundershan S, Fun HK (2013) New pyrazole derivatives containing 1,2,4-triazoles and benzoxazoles as potent antimicrobial and analgesic agents. Eur J Med Chem 62:410–415CrossRefGoogle Scholar
  30. 30.
    Ezabadi IR, Camoutsis C, Zoumpoulakis P et al (2008) Sulfonamide-1,2,4-triazole derivatives as antifungal and antibacterial agents: synthesis, biological evaluation, lipophilicity, and conformational studies. Bioorg Med Chem 16:1150–1161CrossRefGoogle Scholar
  31. 31.
    Sumrra SH, Chohan ZH (2013) In vitro antibacterial antifungal and cytotoxic activities of some triazole Schiff bases and their oxovanadium IV complexes. J Enzyme Inhib Med Chem 28:1291–1299CrossRefGoogle Scholar
  32. 32.
    Almajan GL, Barbuceanu SF, Almajan ER, Draghici C, Saramet G (2009) Synthesis, characterization and antibacterial activity of some triazole Mannich bases carrying diphenylsulfone moieties. Eur J Med Chem 44:3083–3089CrossRefGoogle Scholar
  33. 33.
    Dijk-Wolthuis WNE, Bosch JJK, Hoof AK, Hennink WE (1997) Reaction of dextran with glycidyl methacrylate an unexpected transesterification. Macromolecules 30:3411–3413CrossRefGoogle Scholar
  34. 34.
    Ren X, Liu L, Zhou Y et al (2016) Nanoparticle siRNA against BMI-1 with polyethylenimine-laminarin conjugate for gene therapy in human breast cancer. Bioconjugate Chem 27:66–73CrossRefGoogle Scholar
  35. 35.
    Wang H, Zhu H, Fu W et al (2017) A high strength self-healable antibacterial and anti-inflammatory supramolecular polymer hydrogel. Macromol Rapid Commun 38:1600695CrossRefGoogle Scholar
  36. 36.
    Cao Y, Xiong D, Wang K, Niu Y (2017) Semi-degradbale porous PVA hydrogel scaffold for cartilage repair: evaluation of the initial and cell cultured tribological porperties. J Mech Behav Biomed Mater 68:163–172CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional MaterialsTianjin UniversityTianjinChina

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