Preparation of polycarbonate/gelatine microspheres using a high-voltage electrostatic technique for enhancing the adhesion and proliferation of mesenchymal stem cells

Abstract

In this study, a high-voltage electrostatic technique is introduced to prepare polycarbonate (PC) microspheres in order to design an expansion strategy for the bone marrow mesenchymal stem cells (BMMSCs). The effects of the solution concentration, temperature, nozzle specification and voltage on the sphericity, homogeneity, diameter and sedimentation velocities of the microspheres are investigated. By optimising the preparation parameters, PC microspheres with a diameter of 316.39 μm ± 14.75 μm and porous surface were prepared. The gelatine-modified PC (GEL-PC) microspheres exhibited better hydrophilicity and protein adsorption ability than PC microspheres. Enhancement of the adhesion and proliferation of the BMMSCs can be observed on GEL-PC microspheres after 7 days. Therefore, this study demonstrates that employing a dynamic culture using GEL-PC microspheres is a promising method for deriving BMMSCs.

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Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (Project Nos. 51673186, 51203152, 8167090834 and 51473164), the Program of Scientific Development of Jilin Province (20170520121JH and 20170520141JH), the joint funded program of Chinese Academy of Sciences and Japan Society for the Promotion of Science (GJHZ1519) and the Special Fund for Industrialization of Science and Technology Cooperation between Jilin Province and Chinese Academy of Science (2017SYHZ0021). We thank youdao (https://f.youdao.com) for its linguistic assistance during the preparation of this manuscript.

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Correspondence to Xiaoyu Yang or Yu Wang or Peibiao Zhang.

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Li, C., Li, L., Ma, R. et al. Preparation of polycarbonate/gelatine microspheres using a high-voltage electrostatic technique for enhancing the adhesion and proliferation of mesenchymal stem cells. J Mater Sci 54, 7180–7197 (2019). https://doi.org/10.1007/s10853-018-03200-1

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