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Journal of Materials Science

, Volume 52, Issue 23, pp 13365–13377 | Cite as

A thin PANI and carrageenan–gold nanoparticle film on a flexible gold electrode as a conductive and low-cost platform for sensing in a physiological environment

  • Emanuel Airton de Oliveira Farias
  • Silvania Siqueira Nogueira
  • Aline Márcia de Oliveira Farias
  • Monialine Sousa de Oliveira
  • Maria de Fátima Cardoso Soares
  • Helder Nunes da Cunha
  • José Ribeiro dos Santos Junior
  • Durcilene Alves da Silva
  • Peter Eaton
  • Carla Eiras
Biomaterials

Abstract

In this work, we report the production of a layer-by-layer (LbL) film of gold nanoparticles stabilized with carrageenan (carr-AuNPs) interspersed with a conductive polyaniline (PANI) layer. Conventionally, PANI has poor electroactivity in physiological buffers, limiting its using in electrochemical biosensors. The films were prepared on low cost and easy to manufacture flexible gold electrodes (FEAu). Two adsorption sequences were tested for production of the films—PANI/carr-AuNP and carr-AuNP/PANI. The gold nanoparticle size and colloidal stability were characterized. The films were characterized by cyclic voltammetry, UV–visible spectroscopy and atomic force microscopy. The results showed the synergistic effects of the carr-AuNPs (120 nm) and PANI, which improved both the electrochemical response and the stability of the conductive polymer in physiological medium by three times. The presence of the carr-AuNPs in the film caused a significant increase in roughness of the FEAu-modified electrode compared to that of an unmodified electrode, resulting in an increased active electrode area. Studies of film growth by UV–Vis spectroscopy indicated that the deposition mechanisms of both films involved an auto-regulating adsorption process, with the same amount of material adsorbed in each coating step. The PANI/carr-AUNP film showed considerable improvement in stability and conductivity compared to PANI-only films in the physiological environment, which confers advantages for use as a biosensor.

Notes

Acknowledgements

The work of Peter Eaton was supported by the Fundação para a Ciência e a Tecnologia (FCT) under Grant No. UID/MULTI/04378/2013 - POCI/01/0145/FEDER/007728 with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Emanuel Airton de Oliveira Farias
    • 1
  • Silvania Siqueira Nogueira
    • 1
  • Aline Márcia de Oliveira Farias
    • 1
  • Monialine Sousa de Oliveira
    • 1
  • Maria de Fátima Cardoso Soares
    • 1
  • Helder Nunes da Cunha
    • 2
  • José Ribeiro dos Santos Junior
    • 3
  • Durcilene Alves da Silva
    • 1
  • Peter Eaton
    • 4
  • Carla Eiras
    • 5
  1. 1.Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, CMRVUFPIParnaíbaBrazil
  2. 2.Departamento de Física, CCNUFPITeresinaBrazil
  3. 3.Grupo de BioeletroquímicaUniversidade Federal do PiauíTeresinaBrazil
  4. 4.LAQV-Requimte, Departamento de Química e BioquímicaFaculdade de Ciências da Universidade do PortoPortoPortugal
  5. 5.Laboratório Interdisciplinar de Materiais Avançados, LIMAV, CTUFPITeresinaBrazil

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