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Additive manufacturing of 3D porous alkali-free bioactive glass scaffolds for healthcare applications

  • Biomaterials
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Abstract

This work deals with the fabrication by robocasting of 3D porous scaffolds of an alkali-free bioactive glass composition, FastOs®BG, belonging to the diopside (CaMgSi2O6)–fluorapatite (Ca5(PO4)3F)–tricalcium phosphate (Ca3(PO4)2) system. A glass frit prepared by melt quenching was grinded by dry and wet milling up to getting a suitable combination of particle sizes. The milled frit was then dispersed in aqueous media with the addition of a polycarbonate dispersant, hydroxypropyl methylcellulose (HPMC) as binder and Aristoflex® TAC as gelation agent. The effects of the type and amounts of the processing additives and particle size distribution on the rheological properties of the extrudable pastes were investigated. Printable inks containing 47 vol.% solids with rheological properties tuned to meet the stringent requirements of robocasting technique were obtained. Scaffolds with totally interconnected 3D pore networks and different pore sizes (200, 300 and 500 µm) could be easily fabricated and sintered. The excellent processing and sintering ability resulted in compressive strength values comparable to that of cancellous bone essential for 3D porous scaffolds intended for bone regeneration and tissue engineering applications.

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References

  1. Chan BP, Leong KW (2008) Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J 17(Suppl 4):S467–S479

    Article  Google Scholar 

  2. Hench LL (1991) Bioceramics: from concept to clinic. J Am Ceram Soc 74:1487–1510

    Article  Google Scholar 

  3. O’Brien FJ (2011) Biomaterials & scaffolds for tissue engineering-review. Mater Today 14:88–95

    Article  Google Scholar 

  4. Al-Munajjed AA, Gleeson JP, O’Brien FJ (2008) Development of a collagen calcium-phosphate scaffold as a novel bone graft substitute. Stud Health Technol Inform 133:11–20

    Google Scholar 

  5. Miranda P, Pajares A, Saiz E, Tomsia AP, Guiberteau F (2008) Mechanical properties of calcium phosphate scaffolds fabricated by robocasting. J Biomed Mater Res Part A 85(1):218–227

    Article  Google Scholar 

  6. Li J, Baker BA, Mou X, Ren N, Qiu J, Boughton RI, Liu H (2014) Biopolymer/calcium phosphate scaffolds for bone tissue engineering. Adv Healthc Mater 3:469–484

    Article  Google Scholar 

  7. Fu Q, Saiz E, Rahaman MN, Tomsia AP (2011) Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives. Mater Sci Eng C 31:1245–1256

    Article  Google Scholar 

  8. Jones JR, Ehrenfried LM, Hench LL (2006) Optimising bioactive glass scaffolds for bone tissue engineering. Biomaterials 27:964–973

    Article  Google Scholar 

  9. Chen QZ, Thompson ID, Boccaccini AR (2006) 45S5 Bioglasss-derived glass–ceramic scaffolds for bone tissue engineering. Biomaterials 27:2414–2425

    Article  Google Scholar 

  10. Fu Q, Saiz E, Tomsia AP (2011) Direct ink writing of highly porous and strong glass scaffolds for load-bearing bone defects repair and regeneration. Acta Biomater 7:3547–3554

    Article  Google Scholar 

  11. Xynos ID, Edgar AJ, Buttery LDK, Hench LL, Polak JM (2000) Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. Biochem Biophys Res Commun 276(2):461–465

    Article  Google Scholar 

  12. Xynos ID, Hukkanen MVJ, Buttery LDK, Hench LL, Polak JM (2000) Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering. Calcif Tissue Int 67:321–329

    Article  Google Scholar 

  13. Sabree I, Gough JE, Derby B (2015) Mechanical properties of porous ceramic scaffolds: influence of internal dimensions. Ceram Int 41:8425–8432

    Article  Google Scholar 

  14. Kiziltay A, Marcos-Fernandez A, Roman SJ, Sousa RA, Reis RL, Hasirci V, Hasirci N (2015) Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells. J Tissue Eng Regen Med 9(8):930–942

    Article  Google Scholar 

  15. Loh QL, Choong C (2013) Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Eng Part B 19(6):485–502

    Article  Google Scholar 

  16. Ahn M-K, Moon Y-W, Koha Y-H, Kim H-E (2013) Production of highly porous triphasic calcium phosphate scaffolds with excellent in vitro bioactivity using vacuum-assisted foaming of ceramic suspension (VFC) technique. Ceram Int 39:5879–5885

    Article  Google Scholar 

  17. Wang C, Chen H, Zhu X, Xiao Z, Zhang K, Zhang X (2016) An improved polymeric sponge replication method for biomedical porous titanium scaffolds. Mater Sci Eng C (in press)

  18. Miranda P, Saiz E, Gryn K, Tomsia AP (2006) Sintering and robocasting of beta-tricalcium phosphate scaffolds for orthopaedic applications. Acta Biomater 2(4):457–466

    Article  Google Scholar 

  19. Michna S, Wu W, Lewis JA (2005) Concentrated hydroxyapatite inks for direct-write assembly of 3-D periodic scaffolds. Biomaterials 26:5632–5639

    Article  Google Scholar 

  20. Eqtesadi S, Motealleh A, Miranda P, Lemos A, Rebelo A, Ferreira JMF (2013) A simple recipe for direct writing complex 45S5 Bioglass® 3D scaffolds. Mater Lett 93:68–71

    Article  Google Scholar 

  21. Eqtesadi S, Motealleh A, Miranda P, Pajares A, Lemos A, Ferreira JMF (2014) Robocasting of 45S5 bioactive glass scaffolds for bone tissue engineering. J Eur Ceram Soc 34:113–124

    Article  Google Scholar 

  22. Marques CF, Perera FH, Marote A, Ferreira S, Vieira SI, Olhero S, Miranda P, Ferreira JMF (2017) Biphasic calcium phosphate scaffolds fabricated by direct write assembly: mechanical, anti-microbial and osteoblastic properties. J Eur Ceram Soc 37:359–368

    Article  Google Scholar 

  23. Eqtesadi S, Motealleh A, Pajares A, Miranda P (2015) Effect of milling media on processing and performance of 13–93 bioactive glass scaffolds fabricated by robocasting. Ceram Int 41:1379–1389

    Article  Google Scholar 

  24. Goel A, Kapoor S, Rajagopal RR, Pascual MJ, Kim HW, Ferreira JMF (2012) Alkali-free bioactive glasses for bone tissue engineering: a preliminary investigation. Acta Biomater 8:361–372

    Article  Google Scholar 

  25. Cortez PP, Brito AF, Kapoor S, Correia AF, Atayde LM, Dias-Pereira P, Afonso A, Goel A, Ferreira JMF (2017) The in vivo performance of an alkali-free bioactive glass for bone grafting, FastOs®BG, assessed with an ovine model. J Biomed Mater Res Part B 105:30–38

    Article  Google Scholar 

  26. Kapoor S, Goel A, Tilocca A, Dhuna V, Bhatia G, Dhuna K, Ferreira JMF (2014) Role of glass structure in defining the chemical dissolution behavior, bioactivity and antioxidant properties of zinc and strontium co-doped alkali-free phosphosilicate glasses. Acta Biomater 10:3264–3278

    Article  Google Scholar 

  27. Kapoor S, Goel A, Pascual MJ, Ferreira JMF (2016) Alkali-free bioactive diopside–tricalcium phosphate glass–ceramics for scaffold fabrication: sintering and crystallization behaviours. J Non-Cryst Solids 432:81–89

    Article  Google Scholar 

  28. Weibull W (1951) A statistical distribution function of wide applicability. J Appl Mech 18:93–297

    Google Scholar 

  29. Olhero SM, Ferreira JMF (2004) Influence of particle size distribution on rheology and particle packing of silica-based suspensions. Powder Technol 139:69–75

    Article  Google Scholar 

  30. Tsenga WJ, Wub CH (2003) Sedimentation, rheology and particle-packing structure of aqueous Al2O3 suspensions. Ceram Int 29:821–828

    Article  Google Scholar 

  31. Mueller S, Llewellin EW, Mader HM (2010) The rheology of suspensions of solid particles. Proc R Soc A 466:1201–1228

    Article  Google Scholar 

  32. Hench LL (1998) Bioceramics. J Am Ceram Soc 81:1705–1728

    Article  Google Scholar 

Download references

Acknowledgements

This work was developed in the scope of the CICECO-Aveiro Institute of Materials and funded by FEDER funds through the Operational Programme Competitiveness Factors (COMPETE 2020) and the Portuguese Foundation for Science and Technology (FCT). C. Marques and Hugo R. Fernandes are grateful for the FCT Grants SFRH/BD/78355/2011 and SFRH/BPD/110883/2015, respectively. S. Olhero would like to thank FCT financing from IF/00951/2014.

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Authors and Affiliations

  1. Department of Materials and Ceramics Engineering, CICECO, University of Aveiro, 3810-193, Aveiro, Portugal

    Susana M. Olhero, Hugo R. Fernandes, Catarina F. Marques, Bianca C. G. Silva & José M. F. Ferreira

Authors
  1. Susana M. Olhero
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  2. Hugo R. Fernandes
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  3. Catarina F. Marques
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  4. Bianca C. G. Silva
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  5. José M. F. Ferreira
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Correspondence to Hugo R. Fernandes.

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Olhero, S.M., Fernandes, H.R., Marques, C.F. et al. Additive manufacturing of 3D porous alkali-free bioactive glass scaffolds for healthcare applications. J Mater Sci 52, 12079–12088 (2017). https://doi.org/10.1007/s10853-017-1347-4

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  • DOI: https://doi.org/10.1007/s10853-017-1347-4

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