Skip to main content
SpringerLink
Log in

Evaluation of the pH influence on protein exclusion by restricted access carbon nanotubes coated with bovine serum albumin

  • Original Paper
  • Published:
Journal of Materials Science Aims and scope Submit manuscript

Abstract

This paper’s proposal was to study the pH influence on protein exclusion in restricted access carbon nanotubes (RACNT), obtained by chemical modification of carbon nanotubes (CNT) using concentrated acid oxidizing agent (HNO3—65 % v/v). Oxidized CNT were coated with a layer of bovine serum albumin (BSA), resulting in RACNT–BSA. The isoelectric points of CNT, oxidized CNT, RACNT–BSA, BSA, and cross-linked BSA were determined by potentiometric titration. Percolating the BSA solutions at a concentration of 3 μg mL−1, in a phosphate buffer at a pH range from 2.2 to 7.0, by RACNT–BSA inside SPE cartridges, we studied the interactions among them. Solubilized BSA adsorbed in the RACNT–BSA surface at a pH range near the Ip (3.5–5.2), while in the other studied pH ranges this did not occur, which is mainly governed by electrostatic phenomena.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  1. Iijima S (1991) Helical microtubules of graphitic carbon. Nature 354:56–58. doi:10.1038/354056a0

    Article  Google Scholar 

  2. Terrones M (2003) Science and technology of the twenty-first century: synthesis, properties, and applications of carbon nanotubes. Annu Rev Mater Res 33:419–501. doi:10.1146/annurev.matsci.33.012802.100255

    Article  Google Scholar 

  3. Porter AE, Gass M, Bendall JS, Muller K, Goode A, Skepper JN, Midgley PA, Welland M (2009) Uptake of noncytotoxic acid-treated single-walled carbon nanotubes into the cytoplasm of human macrophage cells. ACS Nano 3:1485–1492. doi:10.1021/nn900416z

    Article  Google Scholar 

  4. Vijayaraj M, Gadiou R, Anselme K, Ghimbeu C, Vix-Guterl C, Orikasa H, Kyotani T, Ittisanronnachai S (2010) The influence of surface chemistry and pore size on the adsorption of proteins on nanostructured carbon materials. Adv Funct Mater 20:2489–2499. doi:10.1002/adfm.201000288

    Article  Google Scholar 

  5. Bohak Z (1977) Biotechnological applications of proteins and enzymes. Academic Press, New York

    Google Scholar 

  6. Dukovic G, Balaz M, Doak P, Berova ND, Zheng M, Mclean RS, Brus LE (2006) Racemic single-walled carbon nanotubes exhibit circular dichroism when wrapped with DNA. J Am Chem Soc 128:9004–9005. doi:10.1021/ja062095w

    Article  Google Scholar 

  7. Ge C, Du J, Zhao L, Wang L, Liu Y, Li D, Yang Y, Zhou R, Zhao Y, Chai Z (2011) Binding of blood proteins to carbon nanotubes reduces cytotoxicity. Proc Natl Acad Sci USA 108:16968–16973. doi:10.1073/pnas.1105270108

    Article  Google Scholar 

  8. Sun Y-P, Fu K, Lin Y, Huang W (2002) Functionalized carbon nanotubes: properties and applications. Acc Chem Res 35:1096–1104. doi:10.1021/ar010160v

    Article  Google Scholar 

  9. Morikawa M, Kuboki Y, Akasaka T, Abe S, Watari F (2012) Effect of pretreatments to carbon nanotubes on protein adsorption. Nano Biomed 4:35–41. doi:10.11344/nano.4.35

    Google Scholar 

  10. Takada T, Kurosaki R, Konno Y, Abe S (2014) Interaction of multi-walled carbon nanotubes with water-soluble proteins: effect of sidewall carboxylation. J Nanosci Nanotechnol 14:3216–3220

    Article  Google Scholar 

  11. Ahmad B, Ahmed MZ, Haq SK, Khan RH (2005) Guanidine hydrochloride denaturation of human serum albumin originates by local unfolding of some stable loops in domain III. Biochim Biophys Acta 1750:93–102. doi:10.1016/j.bbapap.2005.04.001

    Article  Google Scholar 

  12. Guan Y, Zhang H, Wang Y (2014) New insight into the binding interaction of hydroxylated carbon nanotubes with bovine serum albumin. Spectrochim Acta A 124:556–563. doi:10.1016/j.saa.2014.01.058

    Article  Google Scholar 

  13. Barbosa AF, Barbosa VM, Bettini J, Luccas PO, Figueiredo EC (2015) Restricted access carbon nanotubes for direct extraction of cadmium from human serum samples followed by atomic absorption spectrometry analysis. Talanta 131:213–220. doi:10.1016/j.talanta.2014.07.051

    Article  Google Scholar 

  14. Jia Z, Wang Z, Liang J, Wei B, Wu D (1999) Production of short multi-walled carbon nanotubes. Carbon 37:903–906. doi:10.1016/S0008-6223(98)00229-2

    Article  Google Scholar 

  15. Edri E, Regev O (2008) pH effects on BSA-dispersed carbon nanotubes studied by spectroscopy-enhanced composition evaluation techniques. Anal Chem 80:4049–4054. doi:10.1021/ac800124x

    Article  Google Scholar 

  16. Marion MB (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3

    Article  Google Scholar 

  17. Cecchi HM (1999) Fundamentos teóricos e práticos em análise de alimentos. Editora da Unicamp, São Paulo

    Google Scholar 

  18. Jones DB (1941) Factors for converting percentages of nitrogen in foods and feeds into percentages of proteins. US Department of Agriculture, Washington DC

    Google Scholar 

  19. Peters T Jr (1995) All about albumin: biochemistry, genetics, and medical applications. Academic Press, San Diego

    Google Scholar 

  20. Masini JC (1993) Evaluation of neglecting electrostatic interactions on the determination and characterization of the ionizable sites in humic substances. Anal Chim Acta 283:803–810. doi:10.1016/0003-2670(93)85295-U

    Article  Google Scholar 

  21. Soldatov V, Sosinovich Z, Korshunova T, Mironova T (2004) Acid–base properties of ion exchangers. I. Optimising of potentiometric titration of ion exchangers exemplified by carboxylic acid resins. React Funct Polym 58:3–12. doi:10.1016/j.reactfunctpolym.2003.11.003

    Article  Google Scholar 

  22. Mesquita JPd, Martelli PB, Gorgulho HdF (2006) Characterization of copper adsorption on oxidized activated carbon. J Braz Chem Soc 17:1133–1143. doi:10.1590/S0103-50532006000600010

    Article  Google Scholar 

  23. Kim UJ, Furtado CA, Liu X, Chen G, Eklund PC (2005) Raman and IR spectroscopy of chemically processed single-walled carbon nanotubes. J Am Chem Soc 127:15437–15445. doi:10.1021/ja052951o

    Article  Google Scholar 

  24. Ortega F, Pandolfelli V, Rodrigues J, Souza DD (1997) Aspectos da reologia e da estabilidade de suspensões cerâmicas Parte I: fundamentos. Cerâmica 43:5–10. doi:10.1590/S0366-69131997000300003

    Google Scholar 

Download references

Acknowledgements

We would like to acknowledge FAPEMIG, CAPES, and CNPq for the financial support.

Author information

Authors and Affiliations

  1. Chemistry Institute, Federal University of Alfenas-MG, Alfenas, MG, 37130-000, Brazil

    Raphael A. B. Gomes & Pedro O. Luccas

  2. Exact Sciences Institute, Federal University of Alfenas-MG, Alfenas, MG, 37130-000, Brazil

    Cristiana S. de Magalhães

  3. Pharmaceutical Sciences Faculty, Federal University of Alfenas-MG, Alfenas, MG, 37130-000, Brazil

    Eduardo C. de Figueiredo

Authors
  1. Raphael A. B. Gomes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pedro O. Luccas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cristiana S. de Magalhães
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eduardo C. de Figueiredo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cristiana S. de Magalhães.

Ethics declarations

Conflict of interest

We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

Intellectual property

We confirm that we have given due consideration to the protection of intellectual property associated with this work and that there are no impediments to publication, including the timing of publication, with respect to intellectual property. In so doing, we confirm that we have followed the regulations of our institutions concerning intellectual property.

Animal rights

We further confirm that any aspect of the work covered in this manuscript that has involved experimental animals has been conducted with the ethical approval of all relevant bodies and that such approvals are acknowledged within the manuscript.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gomes, R.A.B., Luccas, P.O., de Magalhães, C.S. et al. Evaluation of the pH influence on protein exclusion by restricted access carbon nanotubes coated with bovine serum albumin. J Mater Sci 51, 7407–7414 (2016). https://doi.org/10.1007/s10853-016-9984-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10853-016-9984-6

Keywords

Search

Navigation