Angiogenic potential of boron-containing bioactive glasses: in vitro study
Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO2–Na2O–K2O–MgO–CaO–P2O5–B2O3 (with varying amounts of SiO2 and B2O3) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.
KeywordsVascular Endothelial Growth Factor Bone Marrow Stromal Cell Vascular Endothelial Growth Factor Concentration Vascular Endothelial Growth Factor Secretion Vascular Endothelial Growth Factor Release
The authors acknowledge European Commission funding under the 7th Framework Programme (Marie Curie Initial Training Networks; Grant No. 289958, Bioceramics for bone repair).
- 4.Tatsuyama K, Maezawa Y, Baba H et al (2000) Expression of various growth factors for cell proliferation and cytodifferentiation during fracture repair of bone. Eur J Histochem 44:269–278Google Scholar
- 13.Jung SB, Day DE (2013) Wound care. US 8,535,710 B2Google Scholar
- 14.Jung SB, Day DE (2012) Controlling vessel growth and directionality in mammals and implantable material. US 8,337,875 B2Google Scholar
- 28.Zelzer E, McLean W, Ng Y-S et al (2002) Skeletal defects in VEGF(120/120) mice reveal multiple roles for VEGF in skeletogenesis. Development 129:1893–1904Google Scholar
- 34.Fu Q, Rahaman MN, Bal BS et al (2010) Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation. J Biomed Mater Res A 95:172–179. doi: 10.1002/jbm.a.32823 CrossRefGoogle Scholar