Journal of Materials Science

, Volume 52, Issue 15, pp 8785–8792 | Cite as

Angiogenic potential of boron-containing bioactive glasses: in vitro study

  • P. Balasubramanian
  • L. Hupa
  • B. Jokic
  • R. Detsch
  • A. Grünewald
  • Aldo R. Boccaccini
In Honor of Larry Hench

Abstract

Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO2–Na2O–K2O–MgO–CaO–P2O5–B2O3 (with varying amounts of SiO2 and B2O3) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.

Keywords

Vascular Endothelial Growth Factor Bone Marrow Stromal Cell Vascular Endothelial Growth Factor Concentration Vascular Endothelial Growth Factor Secretion Vascular Endothelial Growth Factor Release 

Notes

Acknowledgements

The authors acknowledge European Commission funding under the 7th Framework Programme (Marie Curie Initial Training Networks; Grant No. 289958, Bioceramics for bone repair).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • P. Balasubramanian
    • 1
  • L. Hupa
    • 2
  • B. Jokic
    • 3
  • R. Detsch
    • 1
  • A. Grünewald
    • 1
  • Aldo R. Boccaccini
    • 1
  1. 1.Department of Materials Science and Engineering, Institute of BiomaterialsUniversity of Erlangen-NurembergErlangenGermany
  2. 2.Johan Gadolin Process Chemistry CentreÅbo Akademi UniversityTurkuFinland
  3. 3.Faculty of Technology and MetallurgyUniversity of BelgradeBelgradeSerbia

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