Mechanochemical synthesis and in vitro studies of chitosan-coated InAs/ZnS mixed nanocrystals
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In this paper, InAs/ZnS mixed nanocrystals were synthesized by dry high-energy milling approach in the first step. The obtained nanocrystals were characterized from structural point of view by X-ray diffraction analysis and Raman spectroscopy, and from the morphological point of view by scanning electron microscopy. In the next step, the nanocrystals were subjected to wet ultra-fine milling in order to obtain a nanosuspension of chitosan-coated InAs/ZnS nanocrystals with bio-imaging properties. The stability of the nanosuspension was examined by zeta potential and particle size distribution measurements. The prepared nanosuspension was stable with high values of zeta potential. Its optical properties were also studied using UV–Vis and PL spectroscopies. The determined fluorescent properties confirming the potential in bio-imaging applications were verified on cancer cell lines Caco-2, HCT116, HeLa, and MCF7.
KeywordsArsenic Chitosan Milling Zeta Potential Particle Size Distribution Measurement
This work was supported by the Slovak Research and Development Agency under the contract No. APVV-14-0103 and by the Slovak Grant Agency VEGA (project 2/0027/14).
- 5.Kumar C (2010) Semiconductor nanomaterials. Wiley, WeinheimGoogle Scholar
- 15.Hallmann S, Fink MJ, Mitchell BS (2011) The mechanochemical formation of functionalized semiconductor nanoparticles for biological, electronic and superhydrophobic surface applications. In: Lu K, Manjooran N, Radovic M, Medvedovski E, Olevsky EA, Li C, Singh G, Chopra N, Pickrell G (eds) Advances in Nanomaterials and Nanostructures, Vol 229. John Wiley & Sons, Inc., Hoboken, NJ. doi: 10.1002/9781118144602.ch13 Google Scholar
- 39.Balaz P, Bujnakova Z, Dutkova E, Balaz M, Zorkovska A, Kovac J et al (2015) Mixed core CdS@ZnS nanocrystals: synthesis, cadmium dissolution and cancer cell management. In: Rene ER, Bhattarai S, Nancharaiah YV, Lens PNL (eds) 4th international conference on research frontiers in chalcogen cycle science and technology. Delft. pp 7–11Google Scholar
- 42.Zucker RM, Daniel KM, Massaro EJ, Karafas SJ, Degn LL, Boyes WK (2013) Detection of silver nanoparticles in cells by flow cytometry using light scatter and far-red fluorescence. Cytom Part A 83:962–972Google Scholar