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Surface modifications to boost sensitivities of electrochemical biosensors using gold nanoparticles/silicon nanowires and response surface methodology approach

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Abstract

This work describes fabrication of a DNA electrochemical sensor utilized of gold nanoparticles/silicon nanowires/indium tin oxide (AuNPs/SiNWs/ITO) as a modified substrate for detection of dengue virus DNA oligomers using methylene blue (MB) as a redox indicator. The response surface methodology (RSM) was applied as one of the advanced optimization methods for fabrication of SiNWs/AuNPs/ITO electrode and immobilization of DNA probes to enhance the sensitivity of DNA detection. Several factors were successfully optimized using RSM, including volume of SiNWs, concentration of dithiopropionic acid (DTPA), volume of AuNPs, DNA probe concentration, and DNA probe immobilization time. RSM approach shows that AuNPs and DNA probe concentration were the prominent factors affecting on the MB current signal and immobilization of DNA probe on AuNPs/SiNWs surface. This new developed sensor was able to discriminate complementary target sequences, noncomplementary and single-base mismatch sequences, for DNA dengue virus detection.

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Acknowledgements

This research was support by the Ministry of Science, Technology and Innovation (MOSTI) with Grant number NND/ND/(1)/TD11-008 under the National Nanotechnology Directorate (NND).

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Authors and Affiliations

  1. Institute of Advanced Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Jahwarhar Izuan Abdul Rashid, Nor Azah Yusof & Reza Hajian

  2. Department of Chemistry and Biology, Centre for Defense Foundation Studies, National Defense University of Malaysia, Sungai Besi Camp, 57000, Kuala Lumpur, Malaysia

    Jahwarhar Izuan Abdul Rashid

  3. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Nor Azah Yusof & Jaafar Abdullah

  4. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia

    Uda Hashim

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  1. Jahwarhar Izuan Abdul Rashid
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  2. Nor Azah Yusof
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Correspondence to Nor Azah Yusof or Reza Hajian.

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Abdul Rashid, J.I., Yusof, N.A., Abdullah, J. et al. Surface modifications to boost sensitivities of electrochemical biosensors using gold nanoparticles/silicon nanowires and response surface methodology approach. J Mater Sci 51, 1083–1097 (2016). https://doi.org/10.1007/s10853-015-9438-6

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  • DOI: https://doi.org/10.1007/s10853-015-9438-6

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