Abstract
A series of N-substituted fulleropyrrolidines containing peptide side chain was synthesized by the quantitative, TFA-mediated deprotection of the corresponding tert-butyl esters. The structures of all compounds were determined by comparative analysis of spectroscopic and spectrometric data. The electrochemical characterization, conducted by cyclic voltammetry at room temperature confirmed slightly attenuated reducibility in comparison to pristine C60 and a weak long-range electron-accepting effect of the Gly3-fragment. The introduction of the peptide subunit led to improved solubility and enabled examination of the antioxidant properties in water environment. A notable radical scavenging activity of the fullerene subunit remained almost unchanged in all compounds. The investigation of the supramolecular self-assembling, performed by the scanning electron microscopy revealed an influence of the side chain, particularly the fraction of the hydrophobic residue, as well as the substrate structure on the final morphology. Most of the compounds underwent highly ordered multi-stage hierarchical assembling to the attractive, flower-shaped supramolecular aggregates during both the precipitation and slow evaporation of the solvent.
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References
Montellano Lόpez A, Mateo-Alonso A, Prato M (2011) Materials chemistry of fullerene C60 derivatives. J Mater Chem 21:1305–1318
Martín N (2006) New challenges in fullerene chemistry. Chem Commun 20:2093–2104
Lai Y-Y, Cheng Y-J, Hsu C-S (2014) Applications of functional fullerene materials in polymer solar cells. Energy Environ Sci 7:1866–1883
Liu Y, Zhao J, Li Z, Mu C, Ma W, Hu H, Jiang K, Lin H, Ade H, Yan H (2014) Aggregation and morphology control enables multiple cases of high-efficiency polymer solar cells. Nat Commun 5:5293. doi:10.1038/ncomms6293
Yan W, Seifermann SM, Pierrat P, Bräse S (2015) Synthesis of highly functionalized C60 fullerene derivatives and their applications in material and life sciences. Org Biomol Chem 13:25–54
Bosi S, Da Ros T, Spalluto G, Prato M (2003) Fullerene derivatives: an attractive tool for biological applications. Eur J Med Chem 38:913–923
Bakry R, Vallant RM, Najam-ul-Haq M, Rainer M, Szabo Z, Huck CW, Bonn GK (2007) Medicinal applications of fullerenes. Int J Nanomed 2:639–649
Partha R, Conyers JL (2009) Biomedical applications of functionalized fullerene-based nanomaterials. Int J Nanomed 4:261–275
Bjelakovic MS, Godjevac DM, Milic DR (2007) Synthesis and antioxidant properties of fullero-steroidal covalent conjugates. Carbon 45:2260–2265
Diederich F, Gómez-López M (1999) Supramolecular fullerene chemistry. Chem Soc Rev 28:263–277
Zhang E-Y, Wang C-R (2009) Fullerene self-assembly and supramolecular nanostructures. Curr Opin Colloid Interface Sci 14:148–156
Babu SS, Möhwald H, Nakanishi T (2010) Recent progress in morphology control of supramolecular fullerene assemblies and its applications. Chem Soc Rev 39:4021–4035
Guldi DM, Zerbetto F, Georgakilas V, Prato M (2005) Ordering fullerene materials at nanometer dimensions. Acc Chem Res 38:38–43
Lens M (2009) Use of fullerenes in cosmetics. Recent Pat Biotechnol 3:118–123
Tam J, Liu J, Yao Z (2013) Effect of microstructure on the antioxidant properties of fullerene polymer solutions. RSC Adv 3:4622–4627
Nakanishi T (2010) Supramolecular soft and hard materials based on self-assembly algorithms of alkyl-conjugated fullerenes. Chem Commun 46:3425–3436
Asanuma H, Li H, Nakanishi T, Möhwald H (2010) Fullerene derivatives that bear aliphatic chains as unusual surfactants: hierarchical self-organization, diverse morphologies, and functions. Chem Eur J 16:9330–9338
Kawauchi T, Kumaki J, Yashima E (2006) Nanosphere and nanonetwork formations of [60]fullerene-end-capped stereoregular poly(methylmethacrylate)s through stereocomplex formation combined with self-assembly of the fullerenes. J Am Chem Soc 128:10560–10567
Gan H, Liu H, Li Y, Gan L, Jiang L, Jiu T, Wang N, He X, Zhu D (2005) Fabrication of fullerene nanotube arrays using a template technique. Carbon 43:205–208
Cassell AM, Asplund CL, Tour JM (1999) Self-assembling supramolecular nanostructures from a C60 derivative: nanorods and vesicles. Angew Chem Int Ed 38:2403–2405
Geng J, Zhou W, Skelton P, Yue W, Kinloch IA, Windle AH, Johnson BFG (2008) Crystal structure and growth mechanism of unusually long fullerene (C60) nanowires. J Am Chem Soc 130:2527–2534
Wang N, Li Y, He X, Gan H, Li Y, Huang C, Xu X, Xiao J, Wang S, Liu H, Zhu D (2006) Synthesis and characterization of a novel electrical and optical-active triads containing fullerene and perylenebisimide units. Tetrahedron 62:1216–1222
Prato M, Bianco A, Maggini M, Scorrano G, Toniolo C, Wudl F (1993) Synthesis and characterization of the first fullerene-peptide. J Org Chem 58:5578–5580
Bianco A, Bertolini T, Crizma M, Valle G, Toniolo C, Maggini M, Scorrano G, Prato M (1997) β-Turn induction by C60-based fulleroproline: synthesis and conformational characterization of Fpr/Pro small peptides. J Pept Res 50:159–170
Sofou P, Elemes Y, Panou-Pomonis E, Stavrakoudis A, Tsikaris V, Sakarellos C, Sakarellos-Daitsiotis M, Maggini M, Formaggio F, Toniolo C (2004) Synthesis of a proline-rich [60]fullerene peptide with potential biological activity. Tetrahedron 60:2823–2828
Tsumoto H, Takahashi K, Suzuki T, Nakagawa H, Kohda K, Miyata N (2008) Preparation of C60-based active esters and coupling of C60 moiety to amines or alcohols. Bioorg Med Chem Lett 18:657–660
Yang J, Alemany LB, Driver J, Hartgerink JD, Barron AR (2007) Fullerene-derivatized amino acids: synthesis, characterization, antioxidant properties, and solid-phase peptide synthesis. Chem Eur J 13:2530–2545
Bianco A (2005) Efficient solid-phase synthesis of fullero-peptides using merrifield strategy. Chem Commun 25:3174–3176
Aroua S, Schweizer WB, Yamakoshi Y (2014) C60 pyrrolidine bis-carboxylic acid derivative as a versatile precursor for biocompatible fullerenes. Org Lett 16:1688–1691
Toniolo C, Bianco A, Maggini M, Scorrano G, Prato M, Marastoni M, Tomatis R, Spisani S, Palú G, Blair ED (1994) A bioactive fullerene peptide. J Med Chem 37:4558–4562
Pantarotto D, Tagmatarchis N, Bianco A, Prato M (2004) Synthesis and biological properties of fullerene-containing amino acids and peptides. Mini Rev Med Chem 4:805–814
Rouse JG, Yang J, Ryman-Rasmussen JP, Barron AR, Monteiro-Riviere NA (2007) Effects of mechanical flexion on the penetration of fullerene amino acid-derivatized peptide nanoparticles through skin. Nano Lett 7:155–160
Yang J, Wang K, Driver J, Yang J, Barron AR (2007) The use of fullerene substituted phenylalanine amino acid as a passport for peptides through cell membranes. Org Biomol Chem 5:260–266
Jennepalli S, Pyne SG, Keller PA (2014) [60]Fullerenyl amino acids and peptides: a review of their synthesis and applications. RSC Adv 4:46383–46398
Sun T, Xu Z (2006) Radical scavenging activities of α-alanine C60 adduct. Bioorg Med Chem Lett 16:3731–3734
Gazit E (2007) Self-assembled peptide nanostructures: the design of molecular building blocks and their technological utilization. Chem Soc Rev 36:1263–1269
Belavtseva EM, Romanova VS, Lapshin AI, Kuleshova EF, Parnes ZN, Vol’pin ME (1996) Electron microscopy study of amino acid derivatives of [60]fullerene in non-aqueous solution. Mendeleev Commun 6:171–173
Vol’pin ME, Belavtseva EM, Romanova VS, Lapshin AI, Aref’eva LI, Parnes ZN (1995) Self-assembling of associates of amino acids and dipeptide derivatives of [60]fullerene in aqueous solution: a study by scanning electron microscopy. Mendeleev Commun 5:129–131
Bjelaković M, Todorović N, Milić D (2012) An approach to nanobioparticles—synthesis and characterization of novel fulleropeptides. Eur J Org Chem 2012:5291–5300
Bjelaković MS, Kop TJ, Vlajić M, Đorđević J, Milić DR (2014) Design, synthesis, and characterization of fullerene-peptide-steroid covalent hybrids. Tetrahedron 70:8564–8570
Lens MB, De Marni E, Gullo R, Citernesi U and Crippa R (2007) Liposomes loaded with fullerene and process for their preparation, WO, 2007043074
Zhang X, Takeuchi M (2009) Controlled fabrication of fullerene C60 into microspheres of nanoplates through porphyrin-polymer-assisted self-assembly. Angew Chem Int Ed 48:9646–9651
Bou R, Codony R, Tres A, Decker EA, Guardiola F (2008) Determination of hydroperoxides in foods and biological samples by the ferrous oxidation-xylenol orange method: a review of the factors that influence the method’s performance. Anal Biochem 377:1–15
Dubois D, Moninot G, Kutner W, Jones MT, Kadish KM (1992) Electroreduction of buckminsterfullerene, C60, in aprotic solvents Solvent, supporting electrolyte, and temperature effects. J Phys Chem 96:7137–7145
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This work was supported by the Ministry of Education, Science and Technological Development (Project No. 172002).
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Bjelaković, M., Kop, T., Maslak, V. et al. Synthesis and characterization of highly ordered self-assembled bioactive fulleropeptides. J Mater Sci 51, 739–747 (2016). https://doi.org/10.1007/s10853-015-9396-z
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DOI: https://doi.org/10.1007/s10853-015-9396-z