Development and characterization of a self-double-emulsifying drug delivery system containing both epigallocatechin-3-gallate and α-lipoic acid
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The present study investigated a self-double-emulsifying drug delivery system (SDEDDS) loaded with epigallocatechin-3-gallate (EGCG) and α-lipoic acid to improve EGCG photostability and possess a sustained release behavior. The long chain solid lipids (cetostearyl alcohol) and macadamia oil were utilized as a carrier to co-deliver the two bioactive ingredients. The prepared EA-SDEDDS for topical application was formulated using modified two-step method and characterized by confocal microscopy, in vitro release, and antioxidant effects. EA-SDEDDS was found to be stable under simulated sunlight for 1 month (at room temperature). In vitro cell culture studies, EA-SDEDDS provided protection for 3t3 fibroblasts that underwent H2O2 oxidative stress due to sustained release property. Cellular antioxidant activity assay indicated that EA-SDEDDS showed anti-oxidative capacity with the same efficacy compared to EGCG-α-lipoic acid solution.
KeywordsEGCG Entrapment Efficiency Solid Lipid Halofantrine Simulated Sunlight
Appreciation is expressed for the financial support of this research from the International Scientific Cooperation Project of the Ministry of Science and Technology of China (No. 2008DFB50060).
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