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Evaluation of biotinylated magnetic nanoparticles for tumour imaging

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Abstract

Target-specific nanoparticles with good stability, dispersibility and targeting properties are very desirable for in vivo applications like diagnosis and therapy. In this work, the potential of covalently biotinylated magnetic nanoparticles for targeting tumours has been analysed by various in vitro and in vivo studies. For quantitatively estimating the amount of biotin bound to the surface of magnetic nanoparticles, 4-Hydroxyazobenzene-2-carboxylic acid dye was used. Varying the pH conditions changes, the mean size and zeta potential and transverse relaxivity slightly, affects the contrast enhancing capability of the synthesized biotinylated magnetic nanoparticles minimally. Cytotoxicity of the synthesized nanoconjugate was analysed in two different cell lines HeLa and A549 by using sulpho-rhodamine B assay and tumour cell-targeting capability was analysed in HeLa cell lines by using confocal microscopy. The results of cytotoxicity analysis combined with intracellular uptake, biodistribution and gamma scintigraphy prove the tumour-targeting potential of the synthesized biocompatible biotinylated magnetic nanoparticle conjugate.

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Acknowledgements

The authors wish to express their thanks and gratitude to Director INMAS, Delhi, for his constant encouragement in carrying out this work. We are deeply thankful to the National Institute of Pharmaceutical Education and Research, Mohali, for supporting some studies. We would also like to show our gratitude to the Department of Chemistry, Panjab University, Chandigarh, and Council of Scientific and Industrial Research, New Delhi.

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Authors and Affiliations

  1. Division of Cyclotron and Radiopharmaceutical Sciences, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, Brig. S.K. Mazumdar Marg, Delhi, 110054, India

    Ram Prakash Chauhan, Rashi Mathur, Narmada Bag, Sweta Singh, Krishna Chuttani & Anil K. Mishra

  2. Department of Chemistry, Panjab University, Chandigarh, 160014, India

    Ram Prakash Chauhan & Gurjaspreet Singh

  3. Division of NMR, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, Brig. S.K. Mazumdar Marg, Delhi, 110054, India

    B. S. Hemanth Kumar

  4. Department of Chemistry, S.V.G.C. Ghumarwin, Bilaspur, 174021, Himachal Pradesh, India

    Ram Prakash Chauhan

  5. Department of Pharmaceuticals, National Institute of Pharmaceutical Education & Research, Mohali, 160062, Punjab, India

    Satyam K. Agrawal

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  1. Ram Prakash Chauhan
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  2. Rashi Mathur
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  3. Gurjaspreet Singh
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  4. Narmada Bag
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  5. Sweta Singh
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  7. B. S. Hemanth Kumar
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  9. Anil K. Mishra
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Correspondence to Rashi Mathur.

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Chauhan, R.P., Mathur, R., Singh, G. et al. Evaluation of biotinylated magnetic nanoparticles for tumour imaging. J Mater Sci 48, 3913–3925 (2013). https://doi.org/10.1007/s10853-013-7193-0

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  • DOI: https://doi.org/10.1007/s10853-013-7193-0

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