Abstract
A novel multilayered composite consisting of regenerated silk fibroin (RSF) nanofiber and carbonated hydroxyapatite (CHA) was fabricated with the combination of electrospinning of RSF aqueous solution and soaking in CaCl2 and Na2HPO4 solutions alternately. The chemical composition and morphologies of RSF/CHA composite were characterized by FT-IR, XRD, TGA, EDX, and SEM. The results showed that such an organic/inorganic composite had an alternate layered structure, while the CHA mineral partly penetrated into the porous RSF mats, which was similar to the structure of natural nacre. By tuning the CHA deposition procedure and RSF electrospinning condition independently, the thickness of each layer of CHA and RSF, as well as the layer numbers of composite, could be easily regulated. For example, the average thickness of CHA layers with 5 and 10 mineralization cycles were 1.63 and 3.19 μm, while 9.03 and 30.12 μm of porous RSF nanofiber layers could be formed with 7 and 24 h electrospinning process, respectively. Thus, it may provide an efficient and general approach to produce a series of inorganic/organic multilayered biomaterials for biomedical engineering.
Similar content being viewed by others
References
Mann S (1988) Nature 332(6160):119–124
Weiner S, Addadi L (1997) J Mater Chem 7(5):689–702
Cheng C, Yang YH, Chen X, Shao ZZ (2008) Chem Commun 43:5511–5513
Sellinger A, Weiss PM, Nguyen A, Lu YF, Assink RA, Gong WL, Brinker CJ (1998) Nature 394(6690):256–260
Wei H, Ma N, Shi F, Wang ZQ, Zhang X (2007) Chem Mater 19(8):1974–1978
Cheng C, Shao ZZ, Vollrath F (2008) Adv Funct Mater 18(15):2172–2179
Landi E, Celotti G, Logroscino G, Tampieri A (2003) J Eur Ceram Soc 23(15):2931–2937
Kikuchi M, Itoh S, Ichinose S, Shinomiya K, Tanaka J (2001) Biomaterials 22(13):1705–1711
Woo KM, Jun J-H, Chen VJ, Seo J, Baek J-H, Ryoo H-M, Kim G-S, Somerman MJ, Ma PX (2007) Biomaterials 28(2):335–343
Matsumura K, Hyon S-H, Nakajima N, Tsutsumi S (2007) J Biomed Mater Res A 82A(2):288–295
Bigi A, Boanini E, Panzavolta S, Roveri N, Rubini K (2002) J Biomed Mater Res 59(4):709–715
Kim HJ, Kim U-J, Kim HS, Li C, Wada M, Leisk GG, Kaplan DL (2008) Bone 42(6):1226–1234
Wang T, Che RC, Li WT, Mi RX, Shao ZZ (2011) Cryst Growth Des 11(6):2164–2171
Wu YD, Cheng C, Yao JR, Chen X, Shao ZZ (2011) Langmuir 27(6):2804–2810
Tsukada M, Goto Y, Freddi G, Shiozaki H, Ishikawa H (1992) J Appl Polym Sci 45(10):1719–1725
Tanahashi M, Matsuda T (1997) J Biomed Mater Res 34(3):305–315
Sato K, Kumagai Y, Tanaka T (2000) J Biomed Mater Res 50(1):16–20
Zhou L, Chen X, Shao ZZ, Huang YF, Knight DP (2005) J Phys Chem B 109:16937–16945
Du C, Jin J, Li Y, Kong X, Wei K, Yao J (2009) Mater Sci Eng C 29(1):62–68
Fan CQ, Li JS, Xu GH, He HL, Ye XJ, Chen YY, Sheng XH, Fu JW, He DN (2010) J Mater Sci 45(21):5814. doi:10.1007/s10853-010-4656-4
Kino R, Ikorna T, Yunoki S, Nagai N, Tanaka J, Asakura T, Munekata M (2007) J Biosci Bioeng 103(6):514–520
Li CM, Jin HJ, Botsaris GD, Kaplan DL (2005) J Mater Res 20(12):3374–3384
Cao H, Chen X, Huang L, Shao ZZ (2009) Mater Sci Eng C 29(7):2270–2274
Minoura N, Aiba SI, Higuchi M, Gotoh Y, Tsukada M, Imai Y (1995) Biochem Biophys Res Commun 208(2):511–516
Wei K, Li Y, Kim K-O, Nakagawa Y, Kim B-S, Abe K, Chen G-Q, Kim I-S (2011) J Biomed Mater Res Part A 97:272–280
Ruan YH, Lin H, Yao JR, Chen ZR, Shao ZZ (2011) Macromol Biosci 11:419–426
Shao ZZ, Chen X, Zhou L (2004) Chinese Patent ZL03142201.2
Furuzono T, Taguchi T, Kishida A, Akashi M, Tamada Y (2000) J Biomed Mater Res 50(3):344–352
Chen X, Shao ZZ, Knight DP, Vollrath F (2002) Acta Chim Sinica 60(12):2203–2208
Zhou W, Chen X, Shao ZZ (2006) Prog Chem 18(11):1514–1522
Xu GF, Aksay IA, Groves JT (2001) J Am Chem Soc 123(10):2196–2203
Landi E, Tampieri A, Celotti G, Vichi L, Sandri M (2004) Biomaterials 25(10):1763–1770
Zou S, Huang J, Best S, Bonfield W (2005) J Mater Sci Mater Med 16(12):1143–1148
Zhang RY, Ma PX (2004) Macromol Biosci 4(2):100–111
Wu Y-S, Lee Y-H, Chang H-C (2009) Mater Sci Eng C 29(1):237–241
Chen J, Chu B, Hsiao BS (2006) J Biomed Mater Res A 79A(2):307–317
Acknowledgements
This work was supported by the National Natural Science Foundation of China (NSFC 20974024 and 21034003) and 973 Project of Chinese Ministry of Science and Technology (No. 2009CB930000).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cao, H., Chen, X., Yao, J. et al. Fabrication of an alternative regenerated silk fibroin nanofiber and carbonated hydroxyapatite multilayered composite via layer-by-layer. J Mater Sci 48, 150–155 (2013). https://doi.org/10.1007/s10853-012-6722-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10853-012-6722-6