Abstract
Enzyme immobilization into solid mesoporous inorganic supports is a promising strategy to enable their use in continuous-flow fixed-bed reactors. In this study, the formation of cross-linked enzyme aggregates (CLEAs) of glucose oxidase (GOx) in the pores of mesocellular foams (MCFs) was investigated. The enzymes can enter the ultra-large cavities connected through the smaller windows, where their agglomeration and cross-linking with glutardialdehyde (GA) will take place. After cross-linking, the diameter of the CLEAs is larger than the diameter of the pore entrance and, thus, the enzymes are trapped in the pores of the support. By varying the experimental parameters, the optimum conditions for the preparation of active and stable immobilized biocatalysts were determined with respect to the resulting activity and to the enzyme loading. It is found that the preparation is preferably performed at pH 5.0, with a time delay between GA and GOx addition of 2 h and a nGA/nGOx-ratio of more than 400.
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Held M, Schmid A, van Beilen JB, Witholt B (2000) Pure Appl Chem 72:1337
Hartmann M (2005) Chem Mater 17:4577
Streb C, Hartmann M (2006) J Porous Mater 13:357
Reetz MT, Zonta A, Simpelkamp J (1995) Angew Chem Int Ed 34:301
Wang YJ, Caruso F (2004) Chem Commun 1528
Yang XY, Li ZQ, Liu B, Klein-Hofmann A, Tian G, Feng YF, Ding Y, Su D, Xiao FS (2006) Adv Mater 18:410
Cao L, van Rantwijk L, Sheldon RA (2000) Org Lett 2:1361
Cao L, van Langen LM, van Rantwijk F, Sheldon RA (2001) J Mol Catal B Enzymatic 11:665
Schoevaart R, Wolbers MW, Golubovic M, Ottens M, Kieboom APG, van Rantwijk F, van der Wielen LAM, Sheldon RA (2004) Biotech Bioeng 87:754
Mateo C, Fernandes B, van Rantwijk F, Stolz A, Sheldon RA (2006) J Mol Catal B Enzymatic 38:154
Schmidt-Winkel P, Lukens WW, Zhao D, Yang P, Chmelka BF, Stucky GD (1999) J Am Chem Soc 121:254
Schmidt-Winkel P, Glinka CJ, Stucky GD (2000) Langmuir 16:356
Schmidt-Winkel P, Lukens WW, Yang P, Margolese DL, Lettow JS, Ying JY, Stucky GD (2000) Chem Mater 12:686
Lettow JS, Han YJ, Schmidt-Winkel P, Yang P, Zhao D, Stucky GD, Ying JY (2000) Langmuir 16:8291
Kim MI, Kim J, Lee J, Jia H, Na HB, Youn JK, Kwak JH, Dohnalkova A, Grate JW, Wang P, Hyeon T, Park HG, Chang HN (2007) Biotech Bioeng 96:210
Lee J, Kim J, Kim J, Jia H, Kim MI, Kwak JH, Jin S, Dohnalkova A, Park HG, Chang HN, Wang P, Grate JW, Hyeon T (2005) Small 1:744
Jung D, Streb C, Hartmann M (2008) Microporous Mesoporous Mater 113:523
Jung D, Paradiso M, Wallacher D, Brandt A, Hartmann M (2009) ChemSusChem 2:161
Bennett EP, Swoboda, Massey V (1965) J Biol Chem 240:2209
Bright H, Appleby M (1969) J Biol Chem 244:3625
Barrett EP, Joyner LG, Halenda PP (1951) J Am Chem Soc 73:373
Lukens WW, Schmidt-Winkel P, Zhao D, Feng J, Stucky GD (1999) Langmuir 15:5403
Hudson S, Magner E, Cooney J, Hodnett BK (2005) J Phys Chem B 109:19496
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Financial support of this work by Elitenetzwerk Bayern is gratefully acknowledged.
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Jung, D., Paradiso, M. & Hartmann, M. Formation of cross-linked glucose oxidase aggregates in mesocellular foams. J Mater Sci 44, 6747–6753 (2009). https://doi.org/10.1007/s10853-009-3917-6
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DOI: https://doi.org/10.1007/s10853-009-3917-6