Preparation of gamma cyclodextrin stabilized solid lipid nanoparticles (SLNS) using stearic acid–γ-cyclodextrin inclusion complex

  • Jeetendra Singh NegiEmail author
  • Pronobesh Chattopadhyay
  • Ashok Kumar Sharma
  • Veerma Ram
Original Article


The inclusion complexation behaviour of higher chain fatty acid, stearic acid (SA) with gamma cyclodextrin has been investigated. The inclusion complex was characterized by FT-IR, 1H NMR, 2D NMR, XRD and DSC techniques. The results showed that the SA molecule was entrapped inside the gamma cyclodextrin cavity. Further, inclusion complex was treated with lopinavir at 85 °C and emulsified with hot water at 85 °C. The resulted nanoemulsion was cooled down to form solid lipid nanoparticles (SLNs) stabilized with gamma cyclodextrin. Prepared SLNs were having average particle size of 212.5 ± 4.8 nm, zeta potential of −19.7 ± 0.66 mV and drug loading of 57.54 ± 0.62 %. The surface characteristics of SLNs were also observed with transmission electron microscopy and atomic force microscopy. Results indicate that inclusion complex of SA and gamma cyclodextrin can be used for SLNs preparation.


Solid lipid nanoparticles (SLNs) Stearic acid–γ-cyclodextrin inclusion complex Gamma cyclodextrin 



Authors are grateful to AIIMS Dehli for providing TEM facility, Wadia Institute of himalayan geology, Dehradun for XRD facility, Sophisticated Analytical Instrumentation Facilities (SAIF), Panjab Univeristy for providing NMR facilities and SMITA lab, IIT Dehli for providing particle size, AFM and DSC facilities. Authors also would like to acknowledge Uttarakhand technical University, Dehradun for their kind support.

Supplementary material

10847_2014_415_MOESM1_ESM.docx (79 kb)
Supplementary material 1 (DOCX 78 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jeetendra Singh Negi
    • 1
    Email author
  • Pronobesh Chattopadhyay
    • 2
  • Ashok Kumar Sharma
    • 3
  • Veerma Ram
    • 1
  1. 1.Department of Pharmaceutical SciencesS Bhagwan Singh PG Institute of Bio-medical Sciences and ResearchDehradunIndia
  2. 2.Division of Pharmaceutical TechnologyDefence Research LaboratoryTezpurIndia
  3. 3.Institute of PharmacyMinistry of HealthAsmaraGovernment of Eritrea

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