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Calix[4]crown-5-ether as a biolinker for immobilization of protein and DNA in fluorescence glass slide chip

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Abstract

Binding affinity between calix[4]crown-5-ether and amino acids have been compared by studying the complexation association constant, and the best value has been obtained from complex of calix[4]crown-5 ether, and HrSOD tagged with Arg and Lys were tested to investigate the effects of specific residues in protein immobilization on calix[4]crown-5-ether. The protein tagged with 9Args has been shown to have much better immobilization potential. Taking advantage of the similar structure of a moiety of guanine base to that of Arg side chain, different homo-oligonucleotides have been immobilized, and it was found that calix[4]crown-5-ether is an appropriate agent in the immobilization of dGTP homo-oligonucleotides. The results demonstrate that calix[4]crown-5-ether on glass slide chip could be applied as an excellently oriented immobilization agent for protein or for DNA microarray designing. It has the ability of single base differentiation in SNP sequence detection.

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Abbreviations

Arg:

Arginine

Lys:

Lysine

G:

Guanine nucleotide

hrSOD:

Human recombinant superoxide dismutase

PBST:

PBS-Tween 20

SNP:

Single nucleotide polymorphism

SSC:

Sodium citrate

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Authors and Affiliations

  1. Chemistry Department, Islamic Azad University, Central Tehran Branch, Tehran, Iran

    Afsaneh Amiri

  2. Biomedical Science Department, Hallym University, Chuncheon, 200-702, South Korea

    Eui Yul Choi

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  1. Afsaneh Amiri
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  2. Eui Yul Choi
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Correspondence to Afsaneh Amiri.

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Amiri, A., Choi, E.Y. Calix[4]crown-5-ether as a biolinker for immobilization of protein and DNA in fluorescence glass slide chip. J Incl Phenom Macrocycl Chem 76, 317–326 (2013). https://doi.org/10.1007/s10847-012-0201-1

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  • DOI: https://doi.org/10.1007/s10847-012-0201-1

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