Effects of solvent, pH and β-cyclodextrin on the fluorescent behaviour of lomustine

  • Hassina Fisli
  • Nadjia Bensouilah
  • Nabila Dhaoui
  • Mohamed Abdaoui
Original Article

Abstract

Fluorescent behaviour of lomustine, a DNA cross-linking agent, was investigated in different solvents, pH and in the presence of β-cyclodextrin (β-CD). The solvents in which fluorescence spectra were observed play a major role in determining the spectral intensity of fluorophore, since it was found to exhibit new fluorescent properties essentially influenced by intermolecular interactions, particularly by intermolecular H-bonding formed with solvents. The pH-dependence profile was typically U-shape with a maximum at pH between 3.51 and 6.58. It was corroborated that the fluorescence emission band of lomustine is significantly intensified in the presence of β-CD. From the changes in the fluorescence spectra, it was concluded that β-CD forms a 1:1 inclusion complex with lomustine and its association constant was calculated.

Keywords

Lomustine β-cyclodextrin Inclusion complex Fluorescence Solvent effect pH effect 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Hassina Fisli
    • 1
  • Nadjia Bensouilah
    • 1
  • Nabila Dhaoui
    • 1
  • Mohamed Abdaoui
    • 1
  1. 1.Laboratoire de Chimie AppliquéeGuelmaAlgeria

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