Skip to main content
SpringerLink
Log in

Purification and Refolding of Cyclodextrin Glycosyltransferase Expressed Escherichia coli

  • Published:
Journal of inclusion phenomena and macrocyclic chemistry Aims and scope Submit manuscript

Abstract

Recombinant DNA technology and protein engineering are currently utilized in the cost-effective production of pharmaceutical and industrial proteins with native conformation. Escherichia coli retains its dominant position as the first choice of host for speed, simplicity and well-established production protocols. However, protein production using recombinant E. coli occasionally encounters complex purification and refolding steps. This paper introduces an efficient scheme for purification andin vitro refolding of industrially important proteins including cyclodextrin glycosyltransferase (CGTase) expressed in recombinant E. coli employing a polycationic amino acid fusion system. Fusion of polycationic amino acids to CGTase allowed purification and refolding of CGTase to be simple and efficient. A novel CGTase production strategy will be discussed by considering recent progress in protein purification and refolding techniques.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. Tonkova (1998) ArticleTitle␣ Enzym. Microb. Technol. 22 678

    Google Scholar 

  2. J.A. Pinto ParticleDe L.L. Campbell (1968) ArticleTitle␣ Biochemistry 7 114

    Google Scholar 

  3. S. Kitahata S. Okada (1974) ArticleTitle␣ Agric. Biol. Chem. 38 2413

    Google Scholar 

  4. R. Jamuna N. Saswathi R. Sheela S.V. Ramakrishna (1993) ArticleTitle␣ Appl. Biochem. Biotechnol. 43 163

    Google Scholar 

  5. S.V. Ramakrishna N. Saswathi R. Sheela R. Jamuna (1994) ArticleTitle␣ Enzym. Microb. Technol. 16 441

    Google Scholar 

  6. L.B. Alves G. Matioli F.F. Moraes ParticleDe G.M. Zanin J.E. Olivo (2002) ArticleTitle␣ J. Incl. Phenom. 44 399

    Google Scholar 

  7. G Georgiou (1995) NoChapterTitle J.L Clerland C.S. Craik (Eds) Principles and Practice of Protein Engineering Butterworths New York 101–127

    Google Scholar 

  8. R.C. Hockney (1994) ArticleTitle␣ Trends. Biotechnol. 12 456

    Google Scholar 

  9. H.-H. Jin N.S. Han D.-H. Kweon Y.-C. Park J.-H. Seo (2001) ArticleTitle␣ J. Microbiol. Biotechnol. 11 91

    Google Scholar 

  10. C.-I. Kim M.-D. Kim Y.-C. Park N.S. Han J.-H. Seo (2000) ArticleTitle␣ J. Microbiol. Biotechnol. 10 632

    Google Scholar 

  11. Y.C. Park C.S. Kim C.-I. Kim K.-H. Choi J.-H. Seo (1997) ArticleTitle␣ J. Microbiol. Biotechnol. 7 323

    Google Scholar 

  12. A. Mukhopadhyay (1997) ArticleTitle␣ Adv. Biochem. Eng. Biotechnol. 56 61

    Google Scholar 

  13. A.P. Middelberg (2002) ArticleTitle␣ Trends. Biotechnol. 20 437

    Google Scholar 

  14. K. Terpe (2003) ArticleTitle␣ Appl. Microbiol. Biotechnol. 60 523

    Google Scholar 

  15. E.R. LaVallie J.M. McCoy (1995) ArticleTitle␣ Curr. Opin. Biotech. 6 501

    Google Scholar 

  16. S.J. Brewer J.M. Sassenfeld (1985) ArticleTitle␣ Trends. Biotechnol. 3 119

    Google Scholar 

  17. H. Dalböge H.H.M. Dahl J. Pedersen J.W. Hansen T. Christensen (1987) ArticleTitle␣ Biotechnology 5 161

    Google Scholar 

  18. J. Zhao C.F. Ford C.E. Glatz M.A. Rougvie S.M. Gendel (1990) ArticleTitle␣ J. Biotechnol. 147 273

    Google Scholar 

  19. M.Q. Niederauer I Suominen M.A. Rougvie C.F. Ford C.E. Glatz (1994) ArticleTitle␣ Biotechnol. Prog. 10 237

    Google Scholar 

  20. M.H. Heng C.E. Glatz (1993) ArticleTitle␣ Biotechnol. Bioeng. 42 333

    Google Scholar 

  21. M.H. Heng C.E. Glatz (1994) ArticleTitle␣ Biotechnol. Bioeng. 44 745

    Google Scholar 

  22. I. Suominen C. Ford D. Stachon H. Heimo M. Niederauer H. Nurmela C. Glatz (1993) ArticleTitle␣ Enzym. Microb. Technol. 15 593

    Google Scholar 

  23. J.R. Luther C.E. Glatz (1994) ArticleTitle␣ Biotechnol. Bioeng. 44 147

    Google Scholar 

  24. J.R. Luther C.E. Glatz (1995) ArticleTitle␣ Biotechnol. Bioeng. 46 62

    Google Scholar 

  25. D.-H. Kweon D.-H. Lee N.-S. Han C.-S. Rha J.-H. Seo (2002) ArticleTitle␣ Biotechnol. Prog. 18 303

    Google Scholar 

  26. A. Mitraki J. King (1989) ArticleTitle␣ Biotechnology 7 190

    Google Scholar 

  27. E.D. Clark (2001) ArticleTitle␣ Curr. Opin. Biotechnol. 12 202

    Google Scholar 

  28. B. Batas J.B. Chaudhuri (1996) ArticleTitle␣ Biotechnol. Bioeng. 50 16

    Google Scholar 

  29. B. Batas C. Schiraldi J.B. Chaudhuri (1999) ArticleTitle␣ J. Biotechnol. 68 149

    Google Scholar 

  30. A. Patchornik M.A. Kraus (1975) ArticleTitle␣ Pure. Appl. Chem. 43 503

    Google Scholar 

  31. C.J. Epstein C.B. Anfinsen (1962) ArticleTitle␣ J. Biol. Chem. 237 2175

    Google Scholar 

  32. V.G. Janolino M.X. Sliwkowski H.E. Swaisgood H.R. Horton (1978) ArticleTitle␣ Arch. Biochem. Biophys. 191 269

    Google Scholar 

  33. T.E. Creighton (1986) NoChapterTitle D.L. Oxender (Eds) Protein Structure, Folding, and Design Alan R Liss NewYork 249–257

    Google Scholar 

  34. A. Negro M. Onisto L. Grassato C. Caenazzo S. Garbisa (1997) ArticleTitle␣ Protein Eng. 10 593

    Google Scholar 

  35. G. Stempfer B. Höll-Neugebauer R. Rudolph (1996) ArticleTitle␣ Nat. Biotechnol. 14 329

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, Korea

    Dae-Hyukkweon Kweon, Sung-Gunkim Kim & Jin-Hoseo Seo

  2. Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, 151-742, Korea

    Jin-Hoseo Seo

Authors
  1. Dae-Hyukkweon Kweon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sung-Gunkim Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin-Hoseo Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Hoseo Seo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kweon, DH., Kim, SG. & Seo, JH. Purification and Refolding of Cyclodextrin Glycosyltransferase Expressed Escherichia coli. J Incl Phenom Macrocycl Chem 50, 37–41 (2004). https://doi.org/10.1007/s10847-003-8836-6

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10847-003-8836-6

Keywords

Search

Navigation