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Journal of Insect Conservation

, Volume 21, Issue 5–6, pp 827–837 | Cite as

Investigating a flower-insect forager network in a mountain grassland community using pollen DNA barcoding

  • Jean-Noël Galliot
  • Dominique Brunel
  • Aurélie Bérard
  • Aurélie Chauveau
  • André Blanchetête
  • Laurent Lanore
  • Anne Farruggia
ORIGINAL PAPER

Abstract

Faced with the decline of pollinators, it is relevant to strengthen our understanding of the whole plant-pollinator web in semi-natural grasslands that serve as refuges for pollinator populations. The aim of this study was to explore the diversity of flower-foraging insects involved in pollen transfer in mountain semi-natural grasslands. Insects actively collecting pollen and/or nectar were caught in spring in six mountain semi-natural grasslands displaying a floristic richness gradient. Individual determinations of insects were made at the finest possible taxonomic scale and pollen loads were removed from the insect body. Using next-generation DNA sequencing, pollens were identified through the ribosomal DNA cistron using the ITS2 database and the ITS plant rDNA cistron sequences from Genbank. A total of 236 flower-foraging insects were collected. Diptera represented 82% of the total catches distantly followed by Hymenoptera (15%) and Apoidea (bees) (11%). Visual observations revealed that Diptera foraged on 16 of the 21 flower species visited by insects. DNA metabarcoding showed that 82% (191) of all of the collected insects were carrying pollen and 44% (104) were carrying two genera of plants or more. Our results demonstrate that Diptera are potential key-pollinators in mountain semi-natural grasslands that cannot be overlooked by the scientific community. However difficulties of taxonomic determination due to severe shortage of experts for Diptera have to be urgently overcome. Further studies on the link between pollen transfer and actual pollination in a global change context are also required. Moreover, our results support the idea that DNA metabarcoding provides accurate information about the plants-insects networks but it also pointed out sensitive issues, especially the necessity to build reliable national barcode databases.

Keywords

Flower-foraging insects Semi-natural grasslands Plant-pollinator interactions DNA barcoding Pollen loads 

Notes

Acknowledgements

The authors thank David Genoud for insect identifications and ecological expertise, Xavier Lair for Syrphidae identifications, Romain Pradinas from National Botanical Conservatory of Massif Central for help on plant identifications and Michel Frain for help on the botanical surveys. The authors also thank Olivier Troquier from INRA-Herbipôle for valuable help with fieldwork logistics and Yoan Gaudron from INRA-UMRH for the Perl scripts.

Supplementary material

10841_2017_22_MOESM1_ESM.docx (619 kb)
Supplementary material 1 (DOCX 618 KB)

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.EPGV US 1279, INRA, CEA, IG-CNGUniversité Paris-SaclayEvryFrance
  2. 2.UE1414 HerbipôleINRALaqueuilleFrance
  3. 3.UMR1213 HerbivoresINRA-VetAgro Sup-Clermont UniversitéSaint-Genès-ChampanelleFrance

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