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Journal of Insect Conservation

, Volume 20, Issue 2, pp 339–343 | Cite as

Playing hide-and-seek with the tiny dragonfly: DNA barcoding discriminates multiple lineages of Nannophya pygmaea in Asia

  • V. L. LowEmail author
  • M. Sofian-Azirun
  • Y. Norma-Rashid
SHORT COMMUNICATION

Abstract

We examined the utility of DNA barcode data for assessing genetic diversity of the tiny dragonfly Nannophya pygmaea Rambur in Asia. Data analyses inferred from the barcode region of cytochrome oxidase subunit I (COI) were performed with Malaysian N. pygmaea, along with the existing COI haplotypes distributed in Asia. We applied four species delimitation analyses [automatic barcode gap discovery (ABGD), generalised mixed yule coalescent (GMYC), poisson tree processes maximum likelihood (PTP_ML) and poisson tree processes simple heuristic solutions (PTP_sh)] to investigate potential lineages in this geographically widespread species. Based on our dataset, we provisionally recognize four distinct lineages or operational taxonomic units of N. pygmaea, which were represented by the taxa from Japan/Korea, China/Laos/Taiwan, Malaysia and Vietnam, respectively. Phylogenetic analyses showed two well-supported assemblages of N. pygmaea: one restricted to the taxa from Malaysia and Vietnam; and the other covering all populations further north (i.e., China, Japan, Korea, Laos and Taiwan). An extraordinarily high degree of genetic distance (up to >12 %) was detected between these two assemblages—suggesting they represent two separate species.

Keywords

COI gene Distinct lineages Odonata Species complex 

Notes

Acknowledgments

This study was supported by the research grants from the University of Malaya (RG209-13SUS) and Malaysian Ministry of Education, Fundamental Research Grant Scheme (FP045-2013A).

References

  1. Angulo A, Ichochea J (2010) Cryptic species complexes, widespread species and conservation: lessons from Amazonian frogs of the Leptodactylus marmoratus group (Anura: Leptodactylidae). Syst Biod 8:357–370. doi: 10.1080/14772000.2010.507264 CrossRefGoogle Scholar
  2. Barley AJ, White J, Diesmos A, Brown RM (2013) The challenge of species delimitation at the extremes: diversification without morphological change in Philippine sun skinks. Evolution 67:3556–3572. doi: 10.1111/evo.12219 CrossRefPubMedGoogle Scholar
  3. Damm S, Schierwater B, Hadrys H (2010) An integrative approach to species discovery in odonates: from character-based DNA barcoding to ecology. Mol Ecol 19:3881–3893. doi: 10.1111/j.1365-294X.2010.04720.x CrossRefPubMedGoogle Scholar
  4. Dijkstra KDB, Kalkman VJ, Dow RA, Stokvis FR, Tol JV (2014) Redefining the damselfly families: a comprehensive molecular phylogeny of Zygoptera (Odonata). Syst Entomol 39:68–96. doi: 10.1111/syen.12035 CrossRefGoogle Scholar
  5. Dow RA, Hämäläinen M, Stokvis FR (2015) Revision of the genus Devadatta Kirby, 1890 in Borneo based on molecular and morphological methods, with descriptions of four new species (Odonata: Zygoptera: Devadattidae). Zootaxa 23(4033):301–349. doi: 10.11646/zootaxa.4033.3.1 CrossRefGoogle Scholar
  6. Drummond AJ, Suchard MA, Xie D, Rambaut A (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol 29:1969–1973. doi: 10.1093/molbev/mss075 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Ezard T, Fujisawa T, Barraclough T (2009) Splits: SPecies’ LImits by Threshold Statistics R package. URL http://r-forge.r-project.org/projects/splits/
  8. Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biol Conserv 61:1–10. doi: 10.1016/0006-3207(92)91201-3 CrossRefGoogle Scholar
  9. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299PubMedGoogle Scholar
  10. Fraser FC (1936) The fauna of British–India including Ceylon and Burma, Odonata, vol 3. Taylor and Francis Ltd., LondonGoogle Scholar
  11. Futahashi R (2011) A revisional study of Japanese dragonflies based on DNA analysis. Tombo 53:67–74Google Scholar
  12. Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755. doi: 10.1093/bioinformatics/17.8.754 CrossRefPubMedGoogle Scholar
  13. Karube H (2009) Nannophya pygmaea. The IUCN red list of threatened species 2009:e.T167187A6312660. http://www.iucnredlist.org/details/167187/0. Accessed 10 Nov 2015
  14. Kekkonen M, Hebert PDN (2014) DNA barcode-based delineation of putative species: efficient start for taxonomic workflows. Mol Ecol Res 14:706–715. doi: 10.1111/1755-0998.12233 CrossRefGoogle Scholar
  15. Kekkonen M, Mutanen M, Kaila L, Nieminen M, Hebert PD (2015) Delineating species with DNA barcodes: a case of taxon dependent method performance in moths. PLoS ONE 10:e0122481. doi: 10.1371/journal.pone.0122481 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Kim KG, Jang SK, Park DW, Hong MY, Oh KH, Kim KY, Hwang JS, Han YS, Kim IS (2007) Mitochondrial DNA sequence variation of the tiny dragonfly, Nannophya pygmaea (Odonata: Libellulidae). Int J Indust Entomol 15:47–58Google Scholar
  17. Low VL, Takaoka H, Adler PH, Ya’cob Z, Norma-Rashid Y, Chen CD, Sofian-Azirun M (2015a) A multi-locus approach resolves the phylogenetic relationships of the Simulium asakoae and Simulium ceylonicum species groups (Diptera: Simuliidae) in Malaysia: evidence for distinct evolutionary lineages. Med Vet Entomol 29:330–337. doi: 10.1111/mve.12120 CrossRefPubMedGoogle Scholar
  18. Low VL, Tay ST, Kho KL, Koh FX, Tan TK, Lim YAL, Panchadcharam C, Ong BL, Norma-Rashid Y, Sofian-Azirun M (2015b) Molecular characterisation of the tick Rhipicephalus microplus in Malaysia: new insights into the cryptic diversity and distinct genetic assemblages throughout the world. Parasit Vectors 8:341. doi: 10.1186/s13071-015-0956-5 CrossRefPubMedPubMedCentralGoogle Scholar
  19. Low VL, Takaoka H, Pramual P, Adler PH, Ya’cob Z, Huang YT, Pham XD, Ramli R, Chen CD, Wannaket A, Sofian-Azirun M (2016) Delineating taxonomic boundaries in the largest species complex of black flies (Simuliidae) in the Oriental Region. Sci Rep 6:20346. doi: 10.1038/srep20346 CrossRefPubMedPubMedCentralGoogle Scholar
  20. Norma-Rashid Y, Mohd-Sofian A, Zakaria-Ismail M (2001) Diversity and distribution of Odonata (dragonflies and damselflies) in the fresh water swamp lake Tasek Bera, Malaysia. Hydrobiologia 459:135–146. doi: 10.1023/A:1012562611307 CrossRefGoogle Scholar
  21. Orr AG (2005) Dragonflies of Peninsular Malaysia and Singapore. Natural History Publication (Borneo), Kota KinabaluGoogle Scholar
  22. Pramual P, Simwisat K, Martin J (2016) Identification and reassessment of the specific status of some tropical freshwater midges (Diptera: Chironomidae) using DNA barcode data. Zootaxa 4072:39–60. doi: 10.11646/zootaxa.4072.1.2 CrossRefGoogle Scholar
  23. Puillandre N, Lambert A, Brouillet S, Achaz G (2012) ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Mol Ecol 21:1864–1877. doi: 10.1111/j.1365-294X.2011.05239.x CrossRefPubMedGoogle Scholar
  24. Rach J, Desalle R, Sarkar IN, Schierwater B, Hadrys H (2008) Character-based DNA barcoding allows discrimination of genera, species and populations in Odonata. Proc Biol Sci 275:237–247. doi: 10.1098/rspb.2007.1290 CrossRefPubMedPubMedCentralGoogle Scholar
  25. Rivera J, Currie DC (2009) Identification of Nearctic black flies using DNA barcodes (Diptera: Simuliidae). Mol Ecol Res 9:224–236. doi: 10.1111/j.1755-0998.2009.02648.x CrossRefGoogle Scholar
  26. Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web-servers. Syst Biol 75:758–771. doi: 10.1080/10635150802429642 CrossRefGoogle Scholar
  27. Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Sinauer Associates, SunderlandGoogle Scholar
  28. Yu X, Xue J, Hämäläinen M, Liu Y, Bu W (2015) A revised classification of the genus Matrona Selys, 1853 using molecular and morphological methods (Odonata: Calopterygidae). Zool J Linn Soc 174:473–486. doi: 10.1111/zoj.12253 CrossRefGoogle Scholar
  29. Zhang J, Kapli P, Pavlidis P, Stamatakis A (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29:2869–2876. doi: 10.1093/bioinformatics/btt499 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Institute of Biological Sciences, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

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