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Canine model of electrical conduction recurrence after radiofrequency catheter ablation constructed by CARTO3 and preliminary application evaluation of DOX-L

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Abstract

Background

Radiofrequency catheter ablation (RFCA) is widely used to treat arrhythmias. However, for atrial fibrillation, the recurrence rate after RFCA is still high. The development of an animal model that mimics the recurrence of electrical conduction after ablation is essential before we can explore the mechanisms involved or develop new therapeutic strategies.

Methods

Eighteen beagles aged 12 to 24 months were randomly assigned to this study. RFCA ablation of the right atrial free wall was performed. Then, electrical block and conduction recovery in the ablation area were evaluated using voltage mapping and pacing tests assisted by CARTO3 system. Finally, liposome doxorubicin (DOX-L) was intravenously injected after ablation to investigate the effect of DOX-L on this animal model.

Results

The conduction block (CB) rates at 5 min after ablation were 16.7%, 83.3%, and 100%, corresponding to 30w, 35w, and 40w power, respectively. However, after 20 min, the rate of CB was 0%, 33.3%, and 75%; thus, the combined success rate of CB and conduction recurrence was 16.7%, 50%, and 25%, respectively. The optimal ablation parameter is 35 W for 20 s, based on the CB rate, REC rate. After 10 days of ablation, the residual conduction recurrence rate was as high as 83.3% in the RFCA alone group, whereas there was no recurrence with RFCA combined with DOX-L treatment.

Conclusions

The novel model accurately simulated the electrical conduction recurrence after cardiac radiofrequency ablation. RFCA combined with DOX-L treatment dramatically reduces the recurrence rate of electrical conduction after ablation.

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Funding

We thank for the support of grants from National Major Science and Technology Infrastructure for Translational Medicine Open Project of Shanghai Jiao Tong University (TMSK-2021–149), Science and Technology Tackling Project of Shanghai Songjiang District Science and Technology Commission (2020SJ288), National Natural Science Foundation of China (81870245, 82070334), Science and Technology Innovation Project of Shanghai Chongming District Science and Technology Commission (CKY2020-5).

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Author notes

  1. Ying Zhuge and Gonghao Li contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Ying Zhuge, Yulong Ge, Xiaoqiang Liu & Fang Wang

  2. Department of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai, China

    Gonghao Li & Fang Wang

  3. Lianyungang Clinical College of Nanjing Medical University, Lianyungang, China

    Gonghao Li

  4. Department of Pharmaceutics, School of Pharmacy, East China University of Science and Technology, Shanghai, China

    Jiajia Zhang

  5. Shanghai Tenth Peoples Hospital, Tongji University, Shanghai, China

    Junshan Wang

  6. Shanghai, People’s Republic of China

    Fang Wang

Authors
  1. Ying Zhuge
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  2. Gonghao Li
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  3. Yulong Ge
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  4. Jiajia Zhang
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  5. Xiaoqiang Liu
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  6. Junshan Wang
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  7. Fang Wang
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Corresponding author

Correspondence to Fang Wang.

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Ethical approval

All animal protocols in this study were approved by the Animal Care and Use Committee, Research Institute of Medicine, Shanghai Jiao Tong University, in accordance with the guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (Publication No. 85–23, revised 1996).

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The authors declare no competing interests.

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Zhuge, Y., Li, G., Ge, Y. et al. Canine model of electrical conduction recurrence after radiofrequency catheter ablation constructed by CARTO3 and preliminary application evaluation of DOX-L. J Interv Card Electrophysiol 66, 1269–1277 (2023). https://doi.org/10.1007/s10840-022-01433-4

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  • DOI: https://doi.org/10.1007/s10840-022-01433-4

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