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Screening and functional analysis of differentially expressed lncRNAs in rapid atrial pacing dog atrial tissue

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Journal of Interventional Cardiac Electrophysiology Aims and scope Submit manuscript

Abstract

Purpose

Atrial fibrillation (AF) is one of the most commonly sustained arrhythmias in clinical practice. Long non-coding RNAs (lncRNAs) are gene regulatory elements involved in the development of several diseases. We aimed to explore the expression characteristics of lncRNAs associated with AF.

Methods

We randomly assigned 12 adult healthy mongrel dogs into a control group and an atrial pacing group. Atrial pacing stimulation was performed at a high frequency of 500 beats per min for 14 consecutive days in the atrial pacing group. HE and Masson staining were used to detect rapid atrial pacing induced atrial fibrosis. Total RNA extraction was performed on dog atrial tissues and was used for high-throughput sequencing of lncRNAs.

Results

A total of 10,310 lncRNAs were detected, and 33 differentially expressed lncRNAs were screened. Among them, 19 lncRNAs were upregulated in the atrial pacing group, and 14 lncRNAs were downregulated. Gene Ontology (GO) classification, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and interaction networks showed that AF-related lncRNAs participate in the regulation of AF in diverse biological processes, cellular components, molecular functions, signaling pathways, and complex interactions with miRNAs and mRNAs. Five differentially expressed lncRNAs were selected for RT-PCR validation, and the verification results were consistent with the results of lncRNA sequencing.

Conclusions

In summary, our study enhances our understanding of the biological functions of AF-related lncRNAs by screening and analyzing differentially expressed lncRNAs, and the results help to enrich the theoretical basis for the treatment of atrial fibrillation.

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Availability of data and material

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

Funding

The present study was approved by the Science & Technology Development Fund of Tianjin Education Commission for Higher Education(No. 2018KJ058).

Author information

Author notes

  1. Wenfeng Shangguan, Lijun Wang and Rukun Cheng contributed equally to this work.

Authors and Affiliations

  1. Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular disease, Department of Cardiology, Tianjin Institute of Cardiology, The Second Hospital of Tianjin Medical University, 23 Pingjiang Road, Hexi, Tianjin, 300211, People’s Republic of China

    Wenfeng Shangguan, Lijun Wang, Rukun Cheng, Tong Liu, Jiageng Cai, Enzhao Liu & Xue Liang

  2. Department of Scientific research, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China

    Baoshuai Zhang

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  1. Wenfeng Shangguan
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Contributions

Xue Liang and Enzhao Liu made substantial contributions to the conception and design of the present study; Lijun Wang and Jiageng Cai acquired the data; Wenfeng Shangguan and Rukun Cheng performed the experiments; Baoshuai Zhang and Tong Liu analyzed and interpreted the data; Xue Liang and Enzhao Liu were involved in drafting the manuscript and revising it critically for important intellectual content. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Enzhao Liu or Xue Liang.

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The authors declare that they have no conflict of interest.

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The present study was approved by the Experimental Animal Administration Committee of Tianjin Medical University and Tianjin Municipal Commission for Experimental Animal Control (IRM-DWLL-2019018).

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Written informed consent for publication was obtained from all participants.

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Shangguan, W., Wang, L., Cheng, R. et al. Screening and functional analysis of differentially expressed lncRNAs in rapid atrial pacing dog atrial tissue. J Interv Card Electrophysiol 61, 375–384 (2021). https://doi.org/10.1007/s10840-020-00824-9

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  • DOI: https://doi.org/10.1007/s10840-020-00824-9

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