Abstract
The comparison of odor functional maps in rodents demonstrates a high degree of inter-individual variability in glomerular activity patterns. There are substantial methodological difficulties in the interindividual assessment of local permutations in the glomerular patterns, since the position of anatomical extracranial landmarks, as well as the size, shape and angular orientation of olfactory bulbs can vary significantly. A new method for defining anatomical coordinates of active glomeruli in the rat olfactory bulb has been developed. The method compares the interindividual odor functional maps and calculates probabilistic maps of glomerular activity with adjustment. This adjustment involves rotation, scaling and shift of the functional map relative to its expected position in probabilistic map, computed according to the anatomical coordinates. The calculation of the probabilistic map of the odorant-specific response compensates for potential anatoamical errors due to individual variability in olfactory bulb dimensions and angular orientation. We show its efficiency on real data from a large animal sample recorded by two-photon calcium imaging in dorsal surface of the rat olfactory bulb. The proposed method with probabilistic map calculation enables the spatial consistency of the effects of individual odorants in different rats to be assessed and allow stereotypical positions of odor-specific clusters in the glomerular layer of the olfactory bulb to be identified.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors express their gratitude to the Advanced Research Foundation for scientific and methodological assistance in organizing research and Southern Federal University Strategic Academic Leadership Program (“Priority 2030”) for providing an experimental base for conducting research.
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This work was supported by the Russian Science Foundation № 22–25–00683, https://rscf.ru/project/22-25-00683/.
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Aleksey E. Matukhno: Methodology, Formal analysis, Investigation, Data curation, Writing - Original Draft. Mikhail V. Petrushan: Conceptualization, Software, Writing - Original Draft. Valery N. Kiroy: Conceptualization, Writing - Review & Editing. Fedor V. Arsenyev: Conceptualization, Resources. Larisa V. Lysenko Data Curation, Writing - Review & Editing, Project administration, Funding acquisition
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Matukhno, A.E., Petrushan, M.V., Kiroy, V.N. et al. The method for assessment of local permutations in the glomerular patterns of the rat olfactory bulb by aligning interindividual odor maps. J Comput Neurosci 51, 433–444 (2023). https://doi.org/10.1007/s10827-023-00858-8
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DOI: https://doi.org/10.1007/s10827-023-00858-8