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Predicted 3D structures of olfactory receptors with details of odorant binding to OR1G1

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Abstract

Olfactory receptors (ORs) are responsible for mediating the sense of smell; they allow humans to recognize an enormous number of odors but the connection between binding and perception is not known. We predict the ensemble of low energy structures for the human OR1G1 (hOR1G1) and also for six other diverse ORs, using the G protein-coupled receptor Ensemble of Structures in Membrane BiLayer Environment complete sampling method that samples 13 trillion different rotations and tilts using four different templates to predict the 24 structures likely to be important in binding and activation. Our predicted most stable structures of hOR1G1 have a salt-bridge between the conserved D3.49 and K6.30 in the D(E)RY region, that we expect to be associated with an inactive form. The hOR1G1 structure also has specific interaction in transmembrane domains (TMD) 3-6 (E3.39 and H6.40), which is likely an important conformational feature for all hORs because of the ~94 to 98 % conservation among all hOR sequences. Of the five ligands studied (nonanal, 9-decen-1-ol, 1-nonanol, camphor, and n–butanal), we find that the 4 expected to bind lead to similar binding energies with nonanol the strongest.

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Acknowledgments

Funding for this project was provided by gifts to the Materials and Process Simulation Center (MSC) at California Institute of Technology, Pasadena, CA. In addition some funding was provided by NIH (R01NS071112 and 1R01NS073115).

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  1. Materials and Process Simulation Center (MC139-74), California Institute of Technology, 1200 E. California Blvd., Pasadena, CA, 91125, USA

    Soo-Kyung Kim & William A. Goddard III

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Kim, SK., Goddard, W.A. Predicted 3D structures of olfactory receptors with details of odorant binding to OR1G1. J Comput Aided Mol Des 28, 1175–1190 (2014). https://doi.org/10.1007/s10822-014-9793-4

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